3次投加H<sub>2</sub>O<sub>2</sub>预氧化对石油污染土壤中石油烃生物降解的研究

徐金兰; 杨正利

doi:10.13205/j.hjgc.202302017

3次投加H₂O₂预氧化对石油污染土壤中石油烃生物降解的研究

doi: 10.13205/j.hjgc.202302017

徐金兰^1,2, ,,
杨正利^1,2

1. 西安建筑科技大学环境与市政工程学院, 西安 710055;
2. 陕西省环境工程重点实验室, 西安 710055

基金项目:

国家自然科学基金项目(51778524)

详细信息

作者简介:
徐金兰(1973-),女,教授,主要研究方向为石油污染土壤修复。xujinlan@xauat.edu.cn

通讯作者:
徐金兰(1973-),女,教授,主要研究方向为石油污染土壤修复。xujinlan@xauat.edu.cn

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出版历程
- 收稿日期: 2022-03-06
- 网络出版日期: 2023-05-25
- 刊出日期: 2023-02-01

IMPACTS OF PETROLEUM HYDROCARBONS BIODEGRADATION IN OIL-CONTAMINATED SOIL AFTER PRE-OXIDATION WITH THREE BATCHS H₂O₂ ADDITION

XU Jinlan^{1,2
, ,},
YANG Zhengli^1,2

1. School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China;
2. Shaanxi Key Laboratory of Environmental Engineering, Xi'an 710055, China

摘要

摘要: 为得到一种高效降解石油烃(TPH)的Fenton预氧化联合生物修复石油污染土壤的修复方式,考察了分3次投加不同浓度H₂O₂预氧化后羟基自由基(·OH)特征、NH⁺₄-N浓度、DOC浓度、脱氢酶及多酚氧化酶活性,以及石油烃去除量。结果表明:5 g石油污染土样体系中,将900 mmol/L的H₂O₂(4.635 mL)分3次投加预氧化后,所产生的·OH最大瞬时强度低,·OH的持续时间短,对石油降解菌杀伤作用少,TPH氧化量较高。生物修复前20 d,NH⁺₄-N浓度消耗量较高(170.45 mg/kg),石油降解菌菌量增长快,长链烷烃C₂₁-C₃₀(22%)和DOC降解率(69%)高,生物修复前期充足NH⁺₄-N浓度的消耗诱导石油降解菌在0~50 d高效降解长链烷烃(42%)。脱氢酶活性和多酚氧化酶活性在20 d达到顶峰,表明微生物的代谢活性增加,石油烃污染物的降解主要发生在此时期。
- Fenton预氧化 /
- NH⁺₄-N /
- 长链烷烃 /
- 生物修复 /
- 酶活性
Abstract: To obtain a remediation method of Fenton pre-oxidation combined with bioremediation of oil-contaminated soil for efficient degradation of petroleum hydrocarbons (TPH), the characteristics of hydroxyl radical (·OH), NH⁺₄-N concentration, DOC concentration, dehydrogenase and polyphenol oxidase activities and TPH removal with dosing different concentration of H₂O₂ by three batches were investigated in this study. The results showed that the maximum instantaneous intensity and duration of ·OH were low, the bacteria destruction was low and TPH oxidation was high after pre-oxidation with 900 mmol/L H₂O₂ (4.635 mL) by three batches dosing. The NH⁺₄-N consumption (170.45 mg/kg) was high and the quantity of hydrocarbon-degrading microorganisms increased rapidly, then long-chain alkanes C₂₁ to C₃₀ (22%) and DOC (69%) degradation were high at the first 20 days in bio-remediation. Therefore, hydrocarbon degraders were induced to degrade long-alkane (42%) in 0 to 50 days by sufficient NH⁺₄-N concentration consumption in the early bio-remediation stage. The activities of dehydrogenase and polyphenol oxidase reached a peak on the 20th day, indicating that the metabolic activity of microorganisms increased, and petroleum hydrocarbon degradation mainly occurred in this period.
- Fenton pre-oxidation /
- NH⁺₄-N /
- long-chain alkane /
- bio-remediation /
- enzyme activity

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