典型生物滞留池细菌群落结构及多样性特征

蔡雯琦; 杨婉婷; 秦华鹏; 银翼翔; 盛颖堃; 王凡

doi:10.13205/j.hjgc.202303009

典型生物滞留池细菌群落结构及多样性特征

doi: 10.13205/j.hjgc.202303009

1. 中山大学大气科学学院南方海洋科学与工程广东省实验室(珠海), 广东珠海 519082;
2. 北京大学深圳研究生院环境与能源学院, 广东深圳 518055;
3. 中山大学化学工程与技术学院, 广东珠海 519082

基金项目:

国家自然科学基金(41603073)

深圳市科技计划项目(JCYJ20200109120416654)

详细信息

作者简介:
蔡雯琦(1999-),女,硕士,主要研究方向为生物地球化学循环。Caiwq3@mail2.sysu.edu.cn

通讯作者:
王凡(1986-),女,副教授,主要研究方向为生物地球化学循环。wangfan25@mail.sysu.edu.cn

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出版历程
- 收稿日期: 2022-05-13
- 网络出版日期: 2023-05-26
- 刊出日期: 2023-03-01

BACTERIAL COMMUNITY STRUCTURE AND DIVERSITY OF TYPICAL BIORETENTION SYSTEMS

1. Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Atmospheric Sciences, Sun Yat-sen University, Zhuhai 519082, China;
2. School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China;
3. School of Chemical Engineering and Technology, Sun Yat-sen University, Zhuhai 519082, China

摘要

摘要: 为了解生物滞留池的细菌群落多样性特征,选取珠海市3个典型生物滞留池为样点,采集19个土壤样本,基于16S rRNA基因高通量测序技术,分析了生物滞留池的细菌群落结构及多样性,探讨了细菌群落与土壤因子的关系及生态功能。结果表明:珠海市生物滞留池的优势菌门(相对丰度>5%)为变形菌门(Proteobacteria)、绿弯菌门(Chloroflexi)、放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria),还含有大量厚壁菌门(Firmicutes)、芽单胞菌门(Gemmatimonadetes)、硝化螺旋菌门(Nitrospirae)、拟杆菌门(Bacteroidetes)、糖化细菌门(Saccharibacteria)、蓝菌门(Cyanobacteria)、浮霉菌门(Planctomycetes)等细菌群落。生物滞留池细菌群落的多样性和丰富度较高,细菌群落结构受生物滞留池中植被类型的影响显著,受土壤深度的影响不显著。土壤因子分析发现,总有机碳(TOC)、总硫(TS)、总氮(TN)、铵氮(NH₄⁺-N)和硝酸盐氮(NO₃^--N)对细菌群落的影响较强,pH值、亚硝酸盐氮(NO₂^--N)的影响较弱;变形菌门的丰度与NH₄⁺-N、NO₃^--N含量呈显著中等负相关,绿弯菌门与TOC、TN呈显著中等负相关,硝化螺旋菌门与pH值呈显著中等正相关,与NO₂^--N呈极显著强正相关,拟杆菌门与NO₃^--N呈显著中等负相关,浮霉菌门与NH₄⁺-N呈显著中等负相关。生态功能预测结果显示,生物滞留池的细菌群落具备较为完善的基础生态功能,包括氨基酸转运与代谢、能量生产与转化、信号传导机制、无机离子转运与代谢、细胞壁/膜/包膜的生成、转录、碳水化合物的运输和代谢等。
- 生物滞留池 /
- 细菌群落结构 /
- 细菌多样性 /
- 高通量测序 /
- 细菌群落生态功能
Abstract: Bioretention system is a green infrastructure which takes advantage of the physical, chemical and biological characteristics of microorganisms and vegetation to regulate the quality and quantity of surface runoff. The bacterial community has a profound influence on the ecological functions of bioretention systems. At present, there are few studies focusing on the structure and diversity of the bacterial community in typical bioretention systems. Nineteen soil samples were collected from three typical bioretention systems in Zhuhai, the structure and diversity of the bacteria community were investigated based on 16S rRNA gene high-throughput sequencing technology, and the relationship between bacterial community and soil environmental factors was discussed, also the predicted ecological functions. The dominant phyla (relative abundance>5%) in three typical bioretention systems were Proteobacteria, Chloroflex, Actinomycetes and Acidobacteria, while the community also contained abundant Firmicutes, Gemmatimonadetes, Nitrospirae, Bacteroidete, Saccharibacteria, Cyanobacteria and Planctomycetes. The diversity and abundance of the bacterial community were relatively high, and the structure of the bacterial community was mainly affected by the vegetation type rather than soil depth. Results indicated that the effects of total organic carbon (TOC), total sulfur (TS), total nitrogen (TN), ammonium-nitrogen (NH₄⁺-N) and nitrate-nitrogen (NO₃^--N) on bacterial community were strong, while those of pH and nitrite-nitrogen (NO₂^--N) were weak. The relative abundance of Proteobacteria was negatively correlated with NH₄⁺-N and NO₃^--N contents, Cloroflexi negatively correlated with TOC and TN, Nitrospirae positively correlated with pH and NO₂^--N, Bacteroides negatively correlated with NO₃^--N, and Plantomycetes negatively correlated with NH₄⁺-N. The bacterial community had a relatively complete set of basic ecological functions, including amino acid transport and metabolism, energy production and conversion, signal transduction mechanisms, inorganic ion transport and metabolism, cell wall/membrane/envelope biogenesis, transcription, carbohydrate transport and metabolism, etc.
- bioretention system /
- bacterial community /
- bacterial diversity /
- high throughput sequencing /
- ecological function

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