锰氧化菌筛选、特性分析及其对砷(Ⅲ)的去除效果

刘粮丰; 王亚楠; 张艳茹; 王华伟; 宋菁; 孙英杰; 史涵; 王清照

doi:10.13205/j.hjgc.202305005

锰氧化菌筛选、特性分析及其对砷(Ⅲ)的去除效果

doi: 10.13205/j.hjgc.202305005

1. 青岛理工大学环境与市政工程学院, 山东青岛 266033;
2. 东营市政务服务中心, 山东东营 257091

基金项目:

山东省高等学校青年创新团队人才引育计划

山东省重点研发计划(2018GSF117030)

国家自然科学基金项目(41907111)

详细信息

作者简介:
刘粮丰(1996-),男,硕士研究生,主要研究方向为污染环境修复。1351168738@qq.com

通讯作者:
王亚楠(1987-),女,博士,副教授,主要研究方向为固体废物污染控制与资源化。wangyanan1005@yeah.net

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出版历程
- 收稿日期: 2022-06-08

SCREENING AND CHARACTERISTIC ANALYSIS OF Mn(Ⅱ) OXIDIZING BACTERIA AND ITS REMOVAL EFFECT ON ARSENIC(Ⅲ)

1. School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266033, China;
2. Dongying Municipal Service Centre, Dongying 257091, China

摘要

摘要: 分离筛选出3株新的高效锰氧化菌,通过谱系鉴定分别命名为Acidovorax facilis WHW-1(敏捷嗜酸菌)、Acinetobacter bereziniae WHW-2 (别雷斯不动杆菌)和Pantoea dispersa WHW-3(分散泛菌),系统研究了3株菌的锰氧化能力及其原位诱导形成的生物锰氧化物(BMO)特性,以及不同初始Mn(Ⅱ)浓度、As(Ⅲ)浓度等因素对 BMO去除As(Ⅲ)的效果与机制。结果表明:1)当细菌接种比为2%,体系初始Mn(Ⅱ)浓度为65 mg/L时,3株锰氧化菌的生长情况良好,WHW-1、WHW-2和WHW-3在培养2周后,诱导产生的 BMO浓度分别达到0.95,0.76,0.53 mg/L;2)当细菌接种比为2%、体系初始Mn(Ⅱ)浓度分别为15,40,65,100 mg/L时,3种细菌诱导形成的BMO在一定范围内随着Mn(Ⅱ)浓度的升高而升高,但Mn(Ⅱ)浓度过高会抑制BMO形成;3)当细菌接种比为2%,Mn(Ⅱ)浓度为65 mg/L,As(Ⅲ)浓度为1~5 mg/L时,原位形成的 BMO对As(Ⅲ)的去除效率均在97%以上,微观分析表明:3种细菌对As(Ⅲ)的去除以吸附作用和铁锰氧化物共沉淀为主。综上可知,筛选出的3株锰氧化菌能够应用于砷污染水环境的修复。
- 锰氧化菌 /
- 生物锰氧化物(BMO) /
- 砷污染 /
- 共沉淀 /
- 吸附作用
Abstract: Three new strains of high-efficient manganese-oxidizing bacteria were isolated, and named Acidovorax facilis WHW-1, Acinetobacter bereziniae WHW-2 and Pantoea dispersa WHW-3 by pedigree identification. The manganese oxidation ability of the three strains and the characteristics of biogenic Mn oxide (BMO) induced in situ were studied systematically. The effect and mechanism of different initial Mn(Ⅱ) concentrations and As(Ⅲ) concentrations on the removal of As(Ⅲ) by BMO were studied. The results showed that: 1)when the inoculation ratio of bacteria was 2% and the initial Mn(Ⅱ) concentration of the system was 65 mg/L, the three manganese-oxidizing bacteria grew well. After two weeks of culturing, the induced BMO concentrations of WHW-1, WHW-2 and WHW-3 reached 0.95, 0.76 and 0.53 mg/L, respectively; 2)when the inoculation ratio of bacteria was 2% and the initial Mn(Ⅱ) concentration of the system was 15, 40, 65 and 100 mg/L, the BMO induced by the three kinds of bacteria increased with the increase of manganese concentration in a certain range, and a too high manganese concentration would inhibit the formation of BMO; 3)when the inoculation ratio of bacteria was 2%, the concentration of Mn(Ⅱ) was 65 mg/L, and the concentration of As(Ⅲ) was 1 to 5 mg/L, the removal efficiency of As(Ⅲ) by BMO formed in situ was 97% above. Microscopic analysis showed that the removal of As (Ⅲ) by three kinds of bacteria was mainly caused by the adsorption and coprecipitation of Fe-Mn oxides. In summary, the three strains of manganese oxidizing bacteria can be used in the remediation of As contaminated water environment.
- Mn(Ⅱ) oxidizing bacteria /
- biogenic Fe-Mn oxide (BMO) /
- arsenic pollution /
- co-precipitation /
- adsorption effect

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