砷胁迫下石油烃降解菌的分离、鉴定及其降解特性

李虹呈; 苏趋; 张武竹; 张耀; 向罗京

doi:10.13205/j.hjgc.202307023

砷胁迫下石油烃降解菌的分离、鉴定及其降解特性

doi: 10.13205/j.hjgc.202307023

李虹呈^1,2,
苏趋^1,2,
张武竹^1,2,3,
张耀^1,2,
向罗京^1,2, ,

1. 湖北省生态环境科学研究院(省生态环境工程评估中心), 武汉 430072;
2. 国家环境保护土壤健康诊断与绿色修复重点实验室, 武汉 430072;
3. 华中农业大学资源与环境学院, 武汉 430070

基金项目:

国家重点研发计划项目（2019YFC1804003）；湖北省省级环保科研项目（2020年）

详细信息

作者简介:
李虹呈,主要研究方向为土壤污染防治。suqu@hbaes.ac.cn

通讯作者:
向罗京(1980-),男,正高级工程师,主要研究方向为土壤污染防治。xlj19651717@163.com

计量
- 文章访问数: 74
- HTML全文浏览量: 14
- PDF下载量: 3
- 被引次数: 0
出版历程
- 收稿日期: 2022-09-14

ISOLATION, IDENTIFICATION AND DEGRADATION CHARACTERISTICS OF STRAINS FOR REMEDIATION OF PETROLEUM HYDROCARBON UNDER ARSENIC STRESS

LI Hongcheng^1,2,
SU Qu^1,2,
ZHANG Wuzhu^1,2,3,
ZHANG Yao^1,2,
XIANG Luojing^{1,2
, ,}

1. Hubei Academy of Environmental Science, Wuhan 430072, China;
2. State Key Laboratory of Soil Health Diagnosis and Green Remediation for Environmental Protection, Wuhan 430072, China;
3. College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China

摘要

摘要: 以湖北省某砷-石油烃复合污染场地的土壤为研究对象，分离纯化砷胁迫下石油烃降解菌，采用16S rDNA测序技术进行菌种鉴定，分析降解菌的生长特性与降解特征，验证降解菌对复合污染土壤的实际修复效果。结果表明：从耐As高效石油烃降解菌株系列中筛选出菌株JYZ-03，其鉴定结果为不动杆菌（Acinetobacter sp.）；菌株JYZ-03最佳生长和降解条件为pH=7、温度30℃、盐度0.1%和初始接种量2%；此条件下菌株JYZ-03对石油烃的降解效率为84.05%，对石油烃各组分降解能力存在差异，难易程度表现为长链烷（C26—C38）>多环芳烃>支链烷烃>中长碳链烷烃（C11—C25），石油烃降解效果显著，具有较好的实际修复效果。该研究丰富了石油烃污染修复功能菌株库，可为复合污染场地修复提供更多选择。
- 石油烃 /
- 砷 /
- 降解菌 /
- 土壤复合污染 /
- 生物修复
Abstract: In this study, petroleum hydrocarbon-degrading strains were isolated and purified from an arsenic-petroleum hydrocarbon composite contaminated site in Hubei Province. 16S rDNA sequencing technology was used to identify the bacteria species. The growth and degradation characteristics of the degrading strain were analyzed, and the actual remediation ability of the degrading strains was tested on the composite contaminated soil. The main conclusions were as follows:Acinetobacter sp. JYZ-03, a highly efficient petroleum hydrocarbon degradation strain resistant to As, was isolated. The optimal growth and degradation conditions were pH of 7, 30℃, salinity of 0.1% and initial inoculation amount of 2%. The degradation rate of petroleum hydrocarbon by strain JYZ-03 was 84.05%. The degradation ability of strain for different petroleum hydrocarbon components was in sequence of long-chain alkane (C26 to C38)>polycyclic aromatic hydrocarbons>branched alkanes>medium and long-chain alkanes (C11 to C25); the actual repairing by the strain achieved a satisfying effect. This study enriches the functional strains for remediation of petroleum hydrocarbon, and provides more options for the remediation of compound-contaminated sites.
- petroleum hydrocarbon /
- arsenic /
- degradation bacteria /
- soil combined pollution /
- bio-remediation

HTML全文

参考文献(43)

[1]	胡迪,李川,董倩倩,等.油田区土壤石油烃组分残留特性研究[J].环境科学, 2014, 35(1):227-232.
[2]	FARRINGTON JW.Oil pollution in the marine environment ii:fates and effects of oil spills[J].Environment, 2014, 56:16-31.
[3]	LIU X Y, JI X Y, LI X W, et al.Migration of petroleum-series contaminants in the soil[J].Soils, 2005, 37:482-486.
[4]	王红旗, 刘新会, 李国学.土壤环境学[B].北京:高等教育出版社, 2007.
[5]	WEI Y, CAI M, ZHU K, et al.Effects of oil pollution on soil moisture[J].Journal of Earth Environment, 2018, 9:266-272.
[6]	OSUJI L C, ONOJAKE C M.Trace heavy metals associated with crude oil:a case study of Ebocha-8 oil-spill-polluted site in Niger Delta, Nigeria[J].Chemistry & Biodiversity, 2004, 1:1708-1715.
[7]	李彦辰.石油烃降解菌的筛选及降解特性研究[D].哈尔滨:哈尔滨工业大学, 2021.
[8]	孙娟, 王宁, 陈宏坤, 等.石油污染土壤微生物群落分布特征[J].石油学报(石油加工), 2021, 37(2):372-383.
[9]	曾秋玲.石油烃降解菌的筛选及降解特性研究[D].大连:大连海事大学, 2020.
[10]	袁林杰, 彭芃, 申泰铭, 等.石油烃降解菌的研究进展[J].生命科学, 2019, 31(10):1088-1097.
[11]	HODSON P V.The Toxicity to fish embryos of pah in crude and refined oils[J].Archives of Environmental Contamination and Toxicology, 2017, 73:12-18.
[12]	周际海,黄荣霞,樊后保,等.污染土壤修复技术研究进展[J].水土保持研究,2016,23(3):366-372.
[13]	孔令姣.石油污染土壤的生物修复及细菌多样性研究[D].兰州:兰州理工大学, 2017.
[14]	CHEN H Q, LI X C, YU X F, et al.A review on technique progresses of microbial diversity in soil ecosystem[J].Earth and Environment, 2018, 46:204-209.
[15]	AL FARRAJ D A, ALKUFEIDY R M, ALKUBAISI N A, et al.Polynuclear aromatic anthracene biodegradation by psychrophilic Sphingomonas sp.,cultivated with tween-80[J].Chemosphere, 2021, 263:128115.
[16]	CHANDRAN P, DAS N.Degradation of diesel oil by immobilized Candida tropicalis and biofilm formed on gravels[J].Biodegradation 2011,22:1181-1189.
[17]	DENG M C, LI J, LIANG F R, et al.Isolation and characterization of a novel hydrocartbon-degrading bacterium Achromobacter sp.HZ01 from the cnude oil-contaminated seawater at the Daya Bay, southemn ChinaC[J].Marine Pollution Bulletin, 2014, 83:79-86.
[18]	GUAN X H, XIE P.Literature review of combined pollution of metal ions and organic pollutants[J].Journal of Civil and Environmental Engineering, 2019, 41:120-128.
[19]	KRONBERG R M, JISKRA M, WIEDERHOLD J G, et al.Methyl mercury formation in hillslope soils of boreal forests:the role of forest harvest and anaerobic microbes[J].Environmental Science & Technology, 2018, 52:367-367.
[20]	LENCHI N, KEBBOUCHE-GANA S, SERVAIS P, et al.Diesel biodegradation capacities and biosurfactant production in saline-alkaline conditions by delftia sp nl1, isolated from an Algerian oilfield[J].Geomicrobiology Journal, 2020, 37:454-466.
[21]	SONG J W, SHEN Q L, WANG L, et al.Effects of Cd, Cu, Zn and their combined action on microbial biomass and bacterial community structure[J].Environmental Pollution, 2018, 243:510-518.
[22]	TZOVOLOU D N, TSAKIROGLOU C D.Experimental study of in situ remediation of lowpermeability soils by BIO VENTING[J].Environmental Engineering and Management Journal, 2018, 17:2645-2656.
[23]	BARSHA M J, DAS S,PAL B Y, et al.Evaluation of arsenic induced toxicity based on arsenic accumulation, translocation and its implications on physio-chemical changes and genomic instability in indica rice (Oryza sativa L.) cultivars[J].Ecotoxicology (London, England), 2020,29(1):13-34.
[24]	GEETS J, COOMAN M D, WITTEBOLLE L, et al.Real-time PCR assay for the simultaneous quantification of nitrifying and denitrifying bacteria in activated sludge[J].Applied Microbiology & Biotechnology, 2007, 75(1):211-221.
[25]	生态环境部.水质可萃取性石油烃(C10-C40)的测定气相色谱法:HJ 894-2017[S].北京:生态环境部,2018.
[26]	生态环境部.土壤和沉积物石油烃(C10-C40)的测定气相色谱法:HJ 1021-2019[S].北京:生态环境部,2018.
[27]	PALANISAMY N, RAMYA J, KUMAR S, et al.Diesel biodegradation capacities of indigenous bacterial species isolated from diesel contaminated soil[J].Journal of Environmental Health Science & Engineering, 2014,12(1):142.
[28]	WHANG L M, LIU P W G, MA C C, et al.Application of rhamnolipid and surfactin for enhanced diesel biodegradation-Effects of pH andammonium addition[J].J Hazard Mater, 2009, 164:1045-1050.
[29]	XIA W, LI J, XIA Y, et al.Optimization of diesel oilbiodegradation in seawater using statistical experimental methodology[J].Water Sci Technol, 2012, 66:1301-1309.
[30]	GREENWOOD P F, WIBROW S, GEORGE S J, et al.Sequential hydro-carbonbiodegradation in a soil from arid coastal Australia treated with oil underlaboratory controlled conditions[J].Org Geochem, 2008, 39:1336-1346.
[31]	LUO Q, ZHANG J G, SHEN X R, et al.Characterization of a noveldiesel oil-degrading pseudomonas sp.strain F4[J].Fresenius Environ Bullet, 2013, 22:689-697.
[32]	MNIF I, SAHNOUN R, ELLOUZE-CHAABOUNI S, et al.Evaluation of B.subtilisSPB1 biosurfactants' potency for diesel-contaminated soil washing:optimization of oil desorption using Taguchi design[J].Environ Sci Pollut Res, 2014, 21:851-861.
[33]	LIU M H, TSAI C F, CHEN B Y.Biodegradation kinetics of diesel in a wind-driven bioventing system[J].The Journal of Korean Society of Soil and Groundwater Environment, 2016, 21:8-15.
[34]	杨茜.石油烃降解菌的筛选及石油污染土壤的生物修复机理研究[D].西安:西安建筑科技大学, 2014.
[35]	WANG Q S, HE M C, WANG Y.Influence of combined pollution of antimony and arsenic on culturable soil microbial populations and enzyme activities[J].Ecotoxicology, 2011, 20:9-19.
[36]	KAUR D, SINGH R P, GUPTA S.Screening and characterization of next-generation biofuels producing bacterial strains[J].Current Microbiology, 2022, 79.
[37]	胡梦杰,钟磊,蔡晓鲜,等.微生物降解石油烃的代谢机制及研究进展[J].环境工程,2023,41(2):234-246.
[38]	SOWANI H, KULKARNI M, ZINJARDE S.Uptake and detoxification of diesel oil by a tropical soil Actinomycete Gordonia amicalis HS-11:Cellular responses and degradation perspectives[J].Environmental Pollution, 2020, 263:114538.
[39]	DAS N, CHANDRAN P.Microbial degradation of petroleum hydrocarbon contaminants:an overview[J].Biotechnol Res Int, 2011, 2011:941810.
[40]	JI Y, MAO G, WANG Y, et al.Structural insights into diversity and n-alkane biodegradation mechanisms of alkane hydroxylases[J].Frontiers in Microbiology, 2013, 4:58.
[41]	ABBASIAN F, LOCKINGTON R, MALLAVARAPU M, et al.A Comprehensive Review of Aliphatic Hydrocarbon Biodegradation by Bacteria[J].Applied Biochemistry and Biotechnology, 2015, 176(3):670-699.
[42]	CABRAL L, GIOVANELLA P, PELLIZZER E P, et al.Microbial communities in petroleum-contaminated sites:structure and metabolisms[J].Chemosphere, 2022, 286:131752.
[43]	PALANISAMY N,RAMYA J,KUMAR S, et al.Diesel biodegradation capacities of indigenous bacterial species isolated from diesel contaminated soil[J].Journal of Environmental Health Science & Engineering, 2014, 12:142.