微藻驱动的好氧甲烷氧化耦合反硝化光生物膜反应器的脱氮性能

付嘉辰; 王静; 赵乙颖; 文辉燕; 安骁; 陈玉成; 周忠波

doi:10.13205/j.hjgc.202308003

微藻驱动的好氧甲烷氧化耦合反硝化光生物膜反应器的脱氮性能

doi: 10.13205/j.hjgc.202308003

付嘉辰¹,
王静²,
赵乙颖¹,
文辉燕¹,
安骁¹,
陈玉成^1,3,
周忠波^1,3, ,

1. 西南大学资源环境学院, 重庆 400715;
2. 长江生态(湖北)科技发展有限责任公司, 武汉 430015;
3. 农村清洁工程重庆市工程研究中心, 重庆 400715

基金项目:

中央高校基本科研业务费专项资金(SWU019031)

西南大学大学生创新训练计划项目(X202110635107)

详细信息

作者简介:
付嘉辰(2000-),男,在读硕士生,主要研究方向为水污染控制技术。fjc1181411817@163.com

通讯作者:
周忠波(1986-),男,教授,主要研究方向为污水处理与资源化。zhouzhongbo-1986@163.com

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- 被引次数: 0
出版历程
- 收稿日期: 2022-07-17
- 网络出版日期: 2023-11-15

NITROGEN REMOVAL PERFORMANCE BY ALGAL-DRIVEN AEROBIC METHANE OXIDATION COUPLED WITH DENITRIFICATION IN A PHOTO-BIOFILM REACTOR

1. College of Resources and Environment, Southwest University, Chongqing 400715, China;
2. Chang Jiang Ecology (Hubei)Technology Development Co., Ltd., Wuhan 430015, China;
3. Chongqing Engineering Research Center of Rural Cleaner Production, Chongqing 400715, China

摘要

摘要: 为解决传统污水处理脱氮过程中额外投加碳源成本高与污水尾水中硝酸盐残留等问题,利用微藻原位产氧形成微氧环境,建立了无须机械供氧的藻菌光生物膜反应器,在不抑制反硝化过程的同时,将好氧甲烷氧化与反硝化耦合,实现污水深度脱氮。探讨了序批式光生物膜反应器的脱氮效率和长期运行的稳定性,并对相关微生物活性和群落组成及功能基因进行分析。结果表明:藻菌光生物膜反应器稳定运行了约50 d,NO^-₃-N去除率稳定在25 mg/(L·d),脱氮效率达到95%,且无亚硝酸盐积累和NH⁺₄-N生成。活性试验显示,反应器中亚硝酸盐反硝化速率高于硝酸盐反硝化速率,且内碳源反硝化对氮的去除有一定积极影响。高通量测序发现,反应器中主要存在真核藻类[小球藻属(Chlorella, 32.45%)和蓝细菌(Pantanalinema_CENA516,3.95%)]、甲烷氧化菌[甲基暖菌属(Methylocaldum, 1.39%)]和反硝化菌[热单胞菌属(Thermomonas, 20.32%)、斯塔普氏菌属(Stappia, 7.24%)和生丝微菌属(Hyphomicrobium, 2.34%)]等功能微生物,宏基因组数据进一步明晰了相应的功能基因组成。综上,将甲烷作为唯一碳源,实现了污水中NO^-₃-N的有效去除,为传统污水脱氮处理提供了一种新范式,对于污水的低碳、高效脱氮处理有重要意义。
- 好氧甲烷氧化 /
- 反硝化 /
- 藻菌共生 /
- 光生物反应器 /
- 脱氮性能
Abstract: To reduce the high cost of external carbon sources addition, and remove the remaining nitrate of the secondary effluent in traditional wastewater treatment, an algae-bacterial photo-biofilm reactor without mechanical oxygen supply was established, by using microalgae to create a micro-oxygen environment, which achieved aerobic methane oxidation coupled with denitrification (AME-D), and finally realized nitrogen removal from wastewater. Here, the long-term performance of the sequencing batch photo-biofilm reactor was investigated, and the related microbial activity and community composition were analyzed. The algae-bacterial photo-biofilm reactor could be operated stably for 50 days, with a stable NO₃^--N removal rate of 25 mg/(L·d), and the nitrogen removal efficiency could reach 95%, which did not cause the pollution of other nitrogen elements. Activity tests showed that the denitrification rate of nitrite was higher than that of nitrate, while the enrichment or accumulation of internal carbon sources could enhance the denitrification process. High-throughput sequencing analysis confirmed the presence of algae [Chlorella (32.45%)] and Cyanobacteria [Pantanalinema_CENA516 (3.95%)], methane-oxidizing bacteria [Methylocaldum (1.39%)] and denitrifying bacteria [Thermomonas (20.32%), Stappia (7.24%), Hyphomicrobium (2.34%)], and the corresponding functional genes were further revealed by metagenomic data analysis. Overall, the photo-biofilm reactor using biogas as the carbon source achieved nitrogen removal from the wastewater without mechanical oxygen supply, which provides a new way for low-carbon and high-efficient treatment of wastewater.
- aerobic methane oxidation /
- denitrification /
- algae-bacterial consortia /
- photobioreactor /
- nitrogen removal

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