有机物浓度变化对复合式SBR厌氧氨氧化系统脱氮性能及菌群结构的影响

门艳; 刘灵婕; 朱雅新; 毕艳孟; 孟凡盛; 于静洁; 王少坡

doi:10.13205/j.hjgc.202308011

有机物浓度变化对复合式SBR厌氧氨氧化系统脱氮性能及菌群结构的影响

doi: 10.13205/j.hjgc.202308011

门艳^1,2,
刘灵婕^1,2,
朱雅新^1,2,
毕艳孟^1,2,
孟凡盛^1,2,
于静洁^1,2,
王少坡^1,2, ,

1. 天津城建大学环境与市政工程学院, 天津 300384;
2. 天津市水质科学与技术重点实验室, 天津 300384

基金项目:

天津市自然科学基金资助项目(18JCZDJC10080)

详细信息

作者简介:
门艳(1998-),女,硕士研究生,主要研究方向为污水生物脱氮。1105688129@qq.com

通讯作者:
王少坡(1975-),男,教授,主要研究方向为污水生物脱氮。wsp@tcu.edu.cn

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- 文章访问数: 53
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- PDF下载量: 4
- 被引次数: 0
出版历程
- 收稿日期: 2022-04-11
- 网络出版日期: 2023-11-15

EFFECT OF ORGANIC MATTER CONCENTRATION VARIATION ON NITROGEN REMOVAL PERFORMANCE AND BACTERIA COMMUNITY STRUCTURE IN A HYBRID SBR ANAMMOX SYSTEM

MEN Yan^1,2,
LIU Lingjie^1,2,
ZHU Yaxin^1,2,
BI Yanmeng^1,2,
MENG Fansheng^1,2,
YU Jingjie^1,2,
WANG Shaopo^{1,2
, ,}

1. School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin 300384, China;
2. Tianjin Key Laboratory of Aquatic Science and Technology, Tianjin 300384, China

摘要

摘要: 采用序批式反应器(SBR)考察有机物(COD)浓度对复合式厌氧氨氧化(Anammox)反应系统脱氮性能及微生物群落结构的影响。结果表明:在进水ρ(COD)为120 mg/L条件下,系统强化脱氮效果最佳,平均总氮去除效率(NRE)和总氮去除率(NRR)分别为95.4%和0.40 kg/(m³·d);当进水ρ(COD)继续增至150 mg/L时,Anammox活性明显下降,NRE和NRR分别降至75.8%和0.32 kg/(m³·d)。16S rRNA高通量测序结果表明,高COD(120~150 mg/L)下,反应体系内反硝化菌相对丰度明显增加,反硝化菌主要存在于絮状污泥中,而絮状污泥中几乎检测不到Anammox菌,Anammox菌在生物膜上则保持着较高的相对丰度(19.1%~32.5%)。这说明适宜的COD浓度可使Anammox与反硝化作用有效耦合,实现SBR系统脱氮功能强化,且反应体系内生物膜的存在是保障复合式Anammox系统稳定脱氮的重要因素。
- 厌氧氨氧化 /
- 反硝化 /
- 生物脱氮 /
- 絮状污泥 /
- 生物膜
Abstract: A sequencing batch reactor (SBR) was used to investigate the effect of organic matter (COD) concentration on nitrogen removal performance and microbial community structure of a hybrid anammox system. The results showed that under the condition of influent COD concentration of 120 mg/L, the system had the best-enhanced nitrogen removal efficiency, and the average total nitrogen removal efficiency (NRE) and total nitrogen removal rate (NRR) were 95.4% and 0.40 kg/(m³·d), respectively; when the influent COD continued to increase to 150 mg/L, the anammox activity decreased obviously, and the NRE and NRR decreased to 75.8% and 0.32 kg/(m³·d), respectively. The results of 16S rRNA high-throughput sequencing showed that the relative abundance of denitrifying bacteria in the system increased significantly at a high COD concentration (120~150 mg/L), and denitrifying bacteria were mainly found in the flocculated sludge, while the Anammox bacteria were almost undetectable in the flocculated sludge, and Anammox bacteria maintained a high relative abundance (19.1%~32.5%) on the biofilm. This indicated that an appropriate COD concentration could achieve the couple of Anammox with denitrification and effectively enhance the function of the SBR system, and the presence of biofilm in the system ensured the nitrogen removal stability of the hybrid Anammox system.
- Anammox /
- denitrification /
- biological nitrogen removal /
- flocculated sludge /
- biofilm

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参考文献(29)

[1]	KUMWIMBA M N,LOTTI T,ŞENEL E,et al.Anammox-based processes:How far have we come and what work remains?A review by bibliometric analysis[J].Chemosphere,2020,238:124627.
[2]	KARTAL B,KUENEN J G,van LOOSDRECHT M C M.Sewage treatment with anammox[J].Science,2010,328(5979):702-703.
[3]	STROUS M,HEIJNEN J J,KUENEN J G,et al.The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms[J].Applied Microbiology and Biotechnology,1998,50(5):589-596.
[4]	XU H,PAERL H W,QIN B,et al.Determining critical nutrient thresholds needed to control harmful cyanobacterial blooms in eutrophic Lake Taihu,China[J].Environmental Science and Technology,2015,49(2):1051-1059.
[5]	GABARRO J,GANIGUE R,GICH F,et al.Effect of temperature on AOB activity of a partial nitritation SBR treating landfill leachate with extremely high nitrogen concentration[J].Bioresource Technology,2012,126:283-289.
[6]	MIAO L,WANG S Y,CAO T H,et al.Advanced nitrogen removal from landfill leachate via Anammox system based on Sequencing Biofilm Batch Reactor (SBBR):effective protection of biofilm[J].Bioresource Technology,2016,220:8-16.
[7]	陈彦霖,郑蕊,隋倩雯,等.厌氧氨氧化处理猪场厌氧消化液的工程应用研究[J].中国给水排水,2022,38(3):7-13.
[8]	ZHANG L,ZHANG S J,PENG Y Z,et al.Nitrogen removal performance and microbial distribution in pilot- and full-scale integrated fixed-biofilm activated sludge reactors based on nitritation-anammox process[J].Bioresource Technology,2015,196:448-453.
[9]	ZHANG F Z,PENG Y Z,MIAO L,et al.A novel simultaneous partial nitrification Anammox and denitrification (SNAD) with intermittent aeration for cost-effective nitrogen removal from mature landfill leachate[J].Chemical Engineering Journal,2017,313:619-628.
[10]	CHEN J,LU Y X,HUANG W,et al.Effect of organic matter on the anammox performance of constructed rapid infiltration systems[J].Environmental Technology,2022,43(12):1770-1782.
[11]	QIN Y J,CAO Y,REN J Y,et al.Effect of glucose on nitrogen removal and microbial community in anammox-denitrification system[J].Bioresource Technology,2017,244(1):33-39.
[12]	van de GRAAF A A,de BRUIJN P,ROBERTSON L A,et al.Autotrophic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor[J].Microbiology,1996,142(8):2187-2196.
[13]	APHA.Standard Methods for the Examination of Water and Wastewater,21st ed[M].Washington,DC:American Public Health Association,2005.
[14]	曹雁.厌氧氨氧化与反硝化协同脱氮及微生物特性研究[D].广州:华南理工大学,2018.
[15]	CAO S B,DU R,LI B K,et al.High-throughput profiling of microbial community structures in an ANAMMOX-UASB reactor treating high-strength wastewater[J].Applied Microbiology and Biotechnology,2016,100(14):6457-6467.
[16]	JIN R C,YANG G F,YU J J,et al.The inhibition of the anammox process:a review[J].Chemical Engineering Journal,2012,197:67-79.
[17]	陈重军,汪瑶琪,姜滢,等.有机物对连续流Anammox脱氮及微生物群落影响[J].中国环境科学,2019,39(12):5049-5055.
[18]	NIU Q G,HE S L,ZHANG Y L,et al.Process stability and the recovery control associated with inhibition factors in a UASB-anammox reactor with a long-term operation[J].Bioresource Technology,2016,203:132-141.
[19]	VADIVELU V M,YUAN Z G,FUX C,et al.The inhibitory effects of free nitrous acid on the energy generation and growth processes of an enriched Nitrobacter culture[J].Environmental Science & Technology,2006,40(14):4442-4448.
[20]	YE L,PIJUAN M,YUAN Z G.The effect of free nitrous acid on the anabolic and catabolic processes of glycogen accumulating organisms[J].Water Research,2010,44(9):2901-2909.
[21]	CHEN X,YUAN H R,ZOU D X,et al.Improving biomethane yield by controlling fermentation type of acidogenic phase in two-phase anaerobic co-digestion of food waste and rice straw[J].Chemical Engineering Journal,2015,273:254-260.
[22]	NI S Q,NI J Y,HU D L,et al.Effect of organic matter on the performance of granular anammox process[J].Bioresource Technology,2012,110:701-705.
[23]	GONZALEZ-MARTINEZ A,RODRIGUEZ-SANCHEZ A,RIVADENEYRA M A,et al.16S rRNA gene-based characterization of bacteria potentially associated with phosphate and carbonate precipitation from a granular autotrophic nitrogen removal bioreactor[J].Applied Microbiology and Biotechnology,2017,101(2):817-829.
[24]	王泓,许荣华,范庆元,等.溶解氧分区控制的一段式自养脱氮工艺的性能及其中微生物的特征[J].环境工程学报,2022,16(3):798-805.
[25]	PODDER A,REINHART D,GOEL R.Nitrogen management in landfill leachate using single-stage anammox process-illustrating key nitrogen pathways under an ecogenomics framework[J].Bioresource Technology,2020,312:123578.
[26]	SPETH D R,GUERRERO-CRUZ S,DUTILH B E,et al.Genome-based microbial ecology of anammox granules in a full-scale wastewater treatment system[J].Nature communications,2016,7(1):11172.
[27]	MARDANOV A V,BELETSKY A V,RAVIN N V,et al.Genome of a novel bacterium “Candidatus Jettenia ecosi” reconstructed from the metagenome of an anammox bioreactor[J].Frontiers in Microbiology,2019,10:2442.
[28]	LI B L,WANG Y,LI J T,et al.The symbiosis of anaerobic ammonium oxidation bacteria and heterotrophic denitrification bacteria in a size-fractioned single-stage partial nitrification/anammox reactor[J].Biochemical Engineering Journal,2019,151:107353.
[29]	严子春,唐瑞祥,吴大冰.有机物对厌氧氨氧化生物膜反应器脱氮效能及微生物群落的影响[J].环境科学学报,2021,41(4):1303-1308.