氯及氯胺消毒方式下管壁生物膜生长特性和群落功能研究

张心悦; 孔赟; 施卫红; 黄咏洲

doi:10.13205/j.hjgc.202401007

氯及氯胺消毒方式下管壁生物膜生长特性和群落功能研究

doi: 10.13205/j.hjgc.202401007

江苏省规划设计集团市政规划与工程设计院, 南京 210019

基金项目:

国家水体污染控制与治理科技重大专项(2014ZX07405002)

详细信息

作者简介:
张心悦(1998-),女,工程师,硕士,主要研究方向为水处理技术。2582391069@qq.com

通讯作者:
孔赟(1987-),男,高级工程师,硕士,主要研究方向为城市水环境治理、排水防涝、海绵城市。307106217@qq.com

计量
- 文章访问数: 73
- HTML全文浏览量: 11
- PDF下载量: 4
- 被引次数: 0
出版历程
- 收稿日期: 2022-10-16
- 网络出版日期: 2024-04-29

GROWTH CHARACTERISTICS AND COMMUNITY FUNCTION OF PIPE WALL BIOFILMS UNDER CHLORINATION/CHLORAMINATION

Municipal Planning and Engineering Design Institute, Jiangsu Provincial Planning and Design Group, Nanjing 210019, China

摘要

摘要: 基于生物膜环状反应器,模拟培养氯消毒和氯胺消毒体系下的管壁生物膜,从生长特性、群落结构和功能丰度等方面探究管壁生物膜特性,研究不同消毒方式(氯和氯胺)胁迫下生物膜的特性差异。结果表明:与氯相比,氯胺消毒下生物膜的单位面积生物量更高,且活菌占比更大,表明在相同浓度水平下,氯比氯胺更能抑制管壁微生物的生长繁殖和细胞活性。氯胺消毒下生物膜的群落丰富度和多样性高于氯消毒,2种消毒方式下的生物膜群落结构和功能丰度存在显著差异。氯消毒下,γ-变形菌纲(平均相对丰度63.8%)占据主要优势,Nevskia属(32.9%)和嗜甲基菌属(20.4%)为优势菌属;氯胺消毒下,α-变形菌纲(53.8%)为最优势菌纲,慢生根瘤菌属(10.4%)为优势菌属。与氯胺相比,氯消毒下生物膜的新陈代谢功能丰度更具优势,尤其是在氨基酸代谢、碳水化合物代谢和脂类代谢关乎有机物利用的功能方面,分别增加36%、41%和48%,为供水管网中的微生物污染控制和以生物膜为前体物的副产物生成控制提供了一定理论基础。
- 管壁生物膜 /
- 氯消毒 /
- 氯胺消毒 /
- 群落结构 /
- 功能丰度
Abstract: Based on the biofilm annular reactor, this study simulated pipe wall biofilm in the chlorine disinfection and chloramine disinfection system. The impact of disinfection(chlorination/chloramination) on the growth characteristics, community composition, and functional abundance of the microbial was explored. The results showed that in comparison with chlorine, the biofilm biomass in the chloramine disinfection system was higher, and the proportion of live bacteria was larger. The community richness and diversity of biofilm under chloramination were higher than that under chlorination. There were significant differences in the community structure of biofilm under the two disinfection methods. Under chlorination, Gammaproteobacteria(63.8%) were predominant, and Nevskia(32.9%) and Methylophilus(20.4%) were the dominant genera; under chloramination, Alphaproteobacteria(53.8%) was the most dominant class, and Bradyrhizobium(10.4%) was the dominant genus. Compared with chloramination, the metabolism functional abundance of the biofilms in the chlorine disinfection system was more advantageous, especially in amino acid metabolism, carbohydrate metabolism, and xenobiotics metabolism. In terms of amino acid metabolism functional pathway, there were significant differences in the functional abundance of tryptophan metabolism, β-alanine metabolism, lysine degradation, and degradation of valine, leucine, and isoleucine for the biofilms under the two disinfection methods.
- pipe wall biofilm /
- chlorination /
- chloramination /
- community structure /
- functional abundance

HTML全文

参考文献(26)

[1]	FISH K,OSBORN A M,BOXALL J B.Biofilm structures (EPS and bacterial communities) in drinking water distribution systems are conditioned by hydraulics and influence discolouration[J].Science of the Total Environment,2017,593-594:571-580.
[2]	潘仁杰.水源切换下管网水质微生物稳定性及控制研究[D].杭州:浙江大学,2020.
[3]	J W,C F H.Contamination potential of drinking water distribution network biofilms[J].Water Science and Technology:A Journal of the International Association on Water Pollution Research,2004,49(11/12):277-286.
[4]	AGGARWAL S,GOMEZ-SMITH C K,JEON Y,et al.Effects of chloramine and coupon material on biofilm abundance and community composition in bench-scale simulated water distribution systems and comparison with full-scale water mains[J].Environmental Science & Technology,2018,52(22):13077-13088.
[5]	WANG H,MASTERS S,HONG Y J,et al.Effect of disinfectant,water age,and pipe material on occurrence and persistence of Legionella,mycobacteria,Pseudomonas aeruginosa,and two amoebas[J].Environmental Science & Technology,2012,46(21):11566-11574.
[6]	WERONIKA G,ANNA P,BEATA K,et al.Influence of pipe material on biofilm microbial communities found in drinking water supply system[J].Environmental Research,2020,196:110433.
[7]	LI X,WANG H,HU C,et al.Characteristics of biofilms and iron corrosion scales with ground and surface waters in drinking water distribution systems[J].Corrosion Science,2015,90(Jan.):331-339.
[8]	CHAN S,PULLERITS K,KEUCKEN A,et al.Bacterial release from pipe biofilm in a full-scale drinking water distribution system[J].Npj Biofilms and Microbiomes,2019,5:9.
[9]	HONG W,SHELDON M,A E M,et al.Effect of disinfectant,water age,and pipe materials on bacterial and eukaryotic community structure in drinking water biofilm[J].Environmental Science & Technology,2014,48(3):1426-1435.
[10]	EDGAR R C.UPARSE:highly accurate OTU sequences from microbial amplicon reads[J].Nat Methods,2013,10(10):996-998.
[11]	WANG Z K,LI L,ARISS R W,et al.The role of biofilms on the formation and decay of disinfection by-products in chloraminated water distribution systems[J].Science of the Total Environment,2021,753:141606-141606.
[12]	CAMPER A K.Involvement of humic substances in regrowth[J].International Journal of Food Microbiology,2004,92(3):355-364.
[13]	CHANDY J P,ANGLES M L.Determination of nutrients limiting biofilm formation and the subsequent impact on disinfectant decay[J].Water Research,2001,35(11):2677-2682.
[14]	XUE Z,LEE W H,COBURN K M,et al.Selective reactivity of monochloramine with extracellular matrix components affects the disinfection of biofilm and detached clusters[J].Environmental Science & Technology,2014,48(7):3832-3839.
[15]	COBURN K M,WANG Q Z,REDISKE D,et al.Effects of extracellular polymeric substance composition on bacteria disinfection by monochloramine:application of MALDI-TOF/TOE-MS and multivariate analysis[J].Environmental Science & Technology,2016,50(17):9197-9205.
[16]	LEE W H,PRESSMAN J G,WAHMAN D G.Three-dimensional free chlorine and monochloramine biofilm penetration:correlating penetration with biofilm activity and viability[J].Environmental Science & Technology,2018,52(4):1889-1898.
[17]	LEE W H,WAHMAN D G,BISHOP P L,et al.Free chlorine and monochloramine application to nitrifying biofilm:comparison of biofilm penetration,activity,and viability[J].Environmental Science & Technology,2011,45(4):1412-1419.
[18]	马凯,杨建军,胡建坤.氯胺给水系统管网水余氯衰减控制策略分析[J].供水技术,2021,15(1):7-12.
[19]	DOUTERELO I,SHARPE R L,BOXALL J B.Influence of hydraulic regimes on bacterial community structure and composition in an experimental drinking water distribution system[J].Water Research,2013,47(2):503-516.
[20]	LI W Y,TAN Q W,ZHOU W,et al.Impact of substrate material and chlorine/chloramine on the composition and function of a young biofilm microbial community as revealed by high-throughput 16S rRNA sequencing[J].Chemosphere,2020,242:125310.
[21]	YAN X,LIN T,WANG X,et al.Effects of pipe materials on the characteristic recognition,disinfection byproduct formation,and toxicity risk of pipe wall biofilms during chlorination in water supply pipelines[J].Water research,2022,210:117980.
[22]	HUANG K L,ZHANG X X,SHI P,et al.A comprehensive insight into bacterial virulence in drinking water using 454 pyrosequencing and Illumina high-throughput sequencing[J].Ecotoxicology and Environmental Safety,2014,109:15-21.
[23]	LEE Y.An evaluation of microbial and chemical contamination sources related to the deterioration of tap water quality in the household water supply system[J].International Journal of Environmental Research and Public Health,2013,10(9):4143-4160.
[24]	LIN W F,YU Z S,ZHANG H X,et al.Diversity and dynamics of microbial communities at each step of treatment plant for potable water generation[J].Water Research,2014,52:218-230.
[25]	SUN W J,LIU W J,CUI L F,et al.Characterization and identification of a chlorine-resistant bacterium,Sphingomonas TS001,from a model drinking water distribution system[J].Science of the Total Environment,2013,458:169-175.
[26]	郭学博.氨基酸类前体物生成含氮消毒副产物的形成过程及控制研究[D].合肥:合肥工业大学,2019.