主流条件下氮负荷对部分亚硝化活性污泥系统中NOB抑制及微生物群落结构的影响

黄文慧; 高佳琦; 李祥; 黄勇; 许佩玲; 叶佳红

doi:10.13205/j.hjgc.202403003

主流条件下氮负荷对部分亚硝化活性污泥系统中NOB抑制及微生物群落结构的影响

doi: 10.13205/j.hjgc.202403003

黄文慧^1,2,
高佳琦^1,2,
李祥^1,2,
黄勇^1,2, ,,
许佩玲^1,2,
叶佳红^1,2

1. 苏州科技大学环境科学与工程学院, 江苏苏州 215009;
2. 苏州科技大学环境生物技术研究所, 江苏苏州 215009

基金项目:

国家自然科学基金项目(51938010)

详细信息

作者简介:
黄文慧(1998-),女,硕士研究生,主要研究方向为废水处理与资源化利用。h792379530@163.com

通讯作者:
黄勇(1958-),男,教授。yhuang_sz@sina.com

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- HTML全文浏览量: 17
- PDF下载量: 7
- 被引次数: 0
出版历程
- 收稿日期: 2023-01-04
- 网络出版日期: 2024-05-31

EFFECT OF NITROGEN LOAD ON NOB INHIBITION IN PARTIAL NITROSATED ACTIVATED SLUDGE SYSTEM UNDER MAINSTREAM CONDITIONS

HUANG Wenhui^1,2,
GAO Jiaqi^1,2,
LI Xiang^1,2,
HUANG Yong^{1,2
, ,},
XU Peiling^1,2,
YE Jiahong^1,2

1. School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China;
2. Institute of Environmental Biotechnology, Suzhou University of Science and Technology, Suzhou 215009, China

摘要

摘要: 部分亚硝化(PN)是厌氧氨氧化(ANAMMOX)获取亚硝酸盐(NO₂^--N) 作为基质的重要途径。然而,市政污水氨氮(NH₄⁺-N)浓度较低且波动频繁,导致难以实现稳定的PN。对此,通过缩短水力停留时间(HRT)的方式启动2个反应器,对比不同氮负荷(NLR)下,PN系统中氮素转化规律及微生物群落结构的变化。结果表明:在低氧环境下,R1的NLR从0.15 kg/(m³·d)提高至0.5 kg/(m³·d),氨氮转化率(ACR)从45%升高至65%,亚氮积累率(NAR)从0升高至95%,表明可以实现PN的快速启动,但是稳定运行60 d后PN出现失稳。然而,在高NLR［0.8~1.2 kg/(m³·d)］条件下,ACR和NAR最高分别可达到68%和85%,且能稳定运行,表明高NLR启动运行更易获得稳定有效的NOB抑制。微生物群落结构进一步表明,随着NLR的提高,R2中NOB的相对丰度远低于R1中NOB的相对丰度;同时,R2中NOB的优势菌逐渐由Nitrospira转变为Nitrolancea。这表明高负荷运行下,NOB菌属类型会发生改变。这为解决主流条件下PN-Anammox工艺的应用瓶颈问题提供了新方法,具有重要的研究意义和应用价值。
- 部分亚硝化(PN) /
- 主流条件 /
- 氮负荷 /
- NOB抑制 /
- 微生物群落结构
Abstract: Partial nitrification (PN) is an important way for anaerobic ammonium oxidation (ANAMMOX) to obtain nitrite (NO₂^--N) as a substrate. However, the concentration of ammonia nitrogen (NH₄⁺-N) in municipal sewage is usually low and fluctuates frequently, making it difficult to achieve a stable PN. In this study, the two reactors were started by shortening the hydraulic retention time (HRT), and the changes in nitrogen transformation and microbial community structure in the PN system were compared under different nitrogen loads (NLR). The results showed that the NLR of R1 increased from 0.15 kg/(m³·d) to 0.5 kg/(m³·d), and the ammonia nitrogen conversion rate (ACR) increased from 45% to 65%, in a hypoxic environment, the nitrous nitrogen accumulation rate (NAR) increased from 0 to 95%, indicating that the rapid start-up of PN could be achieved, but PN became unstable after 60 days of stable operation. However, under the condition of high NLR [0.8~1.2 kg/(m³·d)], ACR and NAR could reach 68% and 85%, and could achieve stable operation, indicating that it was easier to obtain stable and effective NOB inhibition. The microbial community structure further showed that with the increase of NLR, the relative abundance of NOB in R2 was much lower than that in R1; at the same time, the dominant bacteria of NOB in R2 gradually changed from Nitrospira to Nitrolancea. It showed that under high load operation, the type of NOB bacteria changed. This provided a new method for solving the bottleneck problem of the application of the PN-Anammox process under mainstream conditions, which had important research significance and application value.
- partial nitrosation /
- common condition /
- nitrogen load /
- NOB inhibition /
- microbial community structure

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参考文献(35)

[1]	REGMI P, MIILLER M W, HOLGATE B, et al. Control of aeration, aerobic SRT and COD input for mainstream nitritation/denitritation[J]. Water Research, 2014,57:162-171.
[2]	CHRISTENSSON M, EKSTROM S, ANDERSSON C, et al. Experience from start-ups of the first ANITA Mox plants[J]. Water Science and Technology, 2013,67(12):2677-2684.
[3]	GILBERT E M, AGRAWAL S, SCHWARTZ T, et al. Comparing different reactor configurations for Partial Nitritation/Anammox at low temperatures[J]. Water Research, 2015,81:92-100.
[4]	彭永臻,王锦程,李翔晨,等.氮负荷对短程反硝化耦合厌氧氨氧化生物膜系统脱氮性能的影响[J]. 北京工业大学学报, 2021,47(12):1367-1376.
[5]	GUO Q, SHI Z J, XU J L, et al. Inhibition of the partial nitritation by roxithromycin and Cu(Ⅱ)[J]. Bioresource Technology, 2016,214:253-258.
[6]	王晓明,王杰.进水氨氮负荷对污水处理中硝化作用的影响[J].净水技术,2017,36(12):90-93.
[7]	OCHOA J C,COLPRIN J,PALACIOS B, et al. Active heterotrophic and autotrophic biomass distribution between fixed and suspended systems in a hybrid biological reactor[J]. Water Science and Technology, 2002,46 (1/2):397-404.
[8]	JOSS A, DERLON N, CYPRIEN C, et al. Combined nitritation-anammox:advances in understanding process stability[J]. Environment Science and Technology, 2011,45(22):9735-9742.
[9]	GU X D, HUANG Y, HU Y T, et al. Inhibition of nitrite-oxidizing bacteria in automatic recycling PN/ANAMMOX under mainstream conditions[J]. Bioresource Technology, 2021,342:125935.
[10]	QIU S K, HU Y S, LIU R, et al. Start up of partial nitritation-anammox process using intermittently aerated sequencing batch reactor:performance and microbial community dynamics[J]. The Science of the Total Environment, 2019,647:1188-1198.
[11]	LI J L, ZHANG L, PENG Y Z, et al. NOB suppression in partial nitritation-anammox (PNA) process by discharging aged flocs:performance and microbial community dynamics[J]. Chemosphere, 2019,227:26-33.
[12]	APHA. Standard Methods for the Examination of Water and Wastewater[M]. 21st ed. Washington, DC. American Public Health Association, 2005.
[13]	LI X, HUANG Y, YUAN Y, et al. Startup and operating characteristics of an external air-lift reflux partial nitritation-ANAMMOX integrative reactor[J]. Bioresource Technology, 2017,238:657-665.
[14]	SU Y L, PENG Y Z, WANG J, et al. Rapid enrichment of anammox bacteria and transformation to partial denitrification/anammox with nitrification/denitrification sludge[J]. The Science of the Total Environment, 2022,856(Pt 1):158973.
[15]	LI X, YUAN Y, BI Z, et al. Effects of salinity on the denitrification efficiency and community structure of a combined partial nitritation-anaerobic ammonium oxidation process[J]. Bioresource Technology, 2017,249:550-556.
[16]	BLACKBURNE R, YUAN Z, KELLER J. Demonstration of nitrogen removal via nitrite in a sequencing batch reactor treating domestic wastewater[J]. Water Research, 2008,42(8/9):2166-2176.
[17]	LIU G Q, WANG J M. Long-term low DO enriches and shifts nitrifier community in activated sludge[J]. Environment Science and Technology, 2013,47(10):5109-5117.
[18]	NIELSEN J L, NGUYEN H, MEYER R L, et al. Identification of glucose-fermenting bacteria in a full-scale enhanced biological phosphorus removal plant by stable isotope probing[J]. Microbiology, 2012,158(7):1818-1825.
[19]	高逸凡,邹婷,刘霄霄,等.水力停留时间对厌氧氨氧化工艺的影响[J].山东化工,2022,51(11):25-27.
[20]	HAN M, VLAEMINCK S E, AL-OMARI A, et al. Uncoupling the solids retention times of flocs and granules in mainstream deammonification:a screen as effective out-selection tool for nitrite oxidizing bacteria[J]. Bioresource Technology, 2016,221:195-204.
[21]	CAO S B, KOCH K, DU R, et al. Toward mainstream anammox by integrating sidestream treatment[J]. Environmental Science and Technology, 2022,56(15):10553-10556.
[22]	TANG C J, ZHENG P, WANG C H, et al. Suppression of anaerobic ammonium oxidizers under high organic content in high-rate Anammox UASB reactor[J]. Bioresource Technology, 2010,101(6):1762-1768.
[23]	苑宏英,赵鑫,王宏斌,等.氨氮负荷的变化对部分硝化的影响及部分亚硝化的快速启动[J].环境工程学报, 2021,15(8):2748-2758.
[24]	WANG Z Y, ZHENG M, XUE Y, et al. Free ammonia shock treatment eliminates nitrite-oxidizing bacterial activity for mainstream biofilm nitritation process[J]. Chemical Engineering Journal, 2020,393(2):124682.
[25]	WANG J X, LIANG J D, NING D Y, et al. A review of biomass immobilization in anammox and partial nitrification/anammox systems:advances, issues, and future perspectives[J]. The Science of the Total Environment, 2022,821:152792.
[26]	LAURENI M, WEISSBRODT D G, VILLEZ K, et al. Biomass segregation between biofilm and flocs improves the control of nitrite-oxidizing bacteria in mainstream partial nitritation and anammox processes[J]. Water Research, 2019,154:104-116.
[27]	AGRAWAL S, SEUNTJENS D, COCKER P, et al. Success of mainstream partial nitritation/anammox demands integration of engineering, microbiome and modeling insights[J]. Current Opinion in Biotechnology, 2018,50:214-221.
[28]	PICULELL M, SUAREZ C, LI C, et al. The inhibitory effects of reject water on nitrifying populations grown at different biofilm thickness[J]. Water Research, 2016,104:292-302.
[29]	ZHU W, van TENDELOO M, ALLOUL A, et al. Towards mainstream partial nitritation/anammox in four seasons:feasibility of bioaugmentation with stored summer sludge for winter anammox assistance[J]. Bioresource Technology, 2022,347:126619.
[30]	SPIECK E, HARTWIG C, MCCORMACK I, et al. Selective enrichment and molecular characterization of a previously uncultured Nitrospira-like bacterium from activated sludge[J]. Environment Microbiology, 2006,8(3):405-415.
[31]	王朝朝,武新娟,朱书浩,等.低氨氮污水同步亚硝化、厌氧氨氧化耦合异养反硝化(SNAD)工艺启动:运行效能与微生物生态学特性[J]. 中国环境科学, 2023,43(5):2254-2263.
[32]	余轶鹏,张斌,逄超,等.低氮负荷对厌氧氨氧化工艺性能及微生物菌群的影响[J]. 工业用水与废水, 2019,50(6):16-21.
[33]	WANG D, HUANG K, HE X, et al. Varied interspecies interactions between anammox and denitrifying bacteria enhanced nitrogen removal in a single-stage simultaneous anammox and denitrification system[J]. The Science of the Total Environment, 2022,813:152519.
[34]	CAO Y S, van LOOSDRECHTMARKC M, DAIGGER G T. Mainstream partial nitritation-anammox in municipal wastewater treatment:status, bottlenecks, and further studies[J]. Applied Microbiology and Biotechnology, 2017,101(4):1365-1383.
[35]	ZHENG M, LI S L, NI G F, et al. Critical factors facilitating candidatus nitrotoga to be prevalent nitrite-oxidizing bacteria in activated sludge[J]. Environment Science and Technology, 2020,54(23):15414-15423.