长期二硫化碳胁迫下硝化污泥微生物菌群变化

周奇; 韩培培; 侯雅男; 黄聪

doi:10.13205/j.hjgc.202403006

长期二硫化碳胁迫下硝化污泥微生物菌群变化

doi: 10.13205/j.hjgc.202403006

周奇¹,
韩培培¹,
侯雅男²,
黄聪^2, ,

1. 天津科技大学, 天津 300457;
2. 中国科学院天津工业生物技术研究中心, 天津 300308

基金项目:

天津市合成生物技术创新能力提升行动——环境与能源安全合成微生物组(TSBICIP-CXRC-007)

详细信息

作者简介:
周奇(1997-),女,硕士研究生,主要研究方向为污水资源化利用。zhouqi1567@163.com

通讯作者:
黄聪(1984-),男,副研究员,主要研究方向为工业废水处理技术。huangc@tib.cas.cn

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- 文章访问数: 48
- HTML全文浏览量: 6
- PDF下载量: 4
- 被引次数: 0
出版历程
- 收稿日期: 2023-03-30
- 网络出版日期: 2024-05-31

CHANGES IN MICROBIAL COMMUNITY OF NITRIFYING SLUDGE UNDER LONG-TERM CARBON DISULFIDE STRESS

1. Tianjin University of Science and Technology, Tianjin 300457, China;
2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China

摘要

摘要: 我国每年约有近百万吨二硫化碳(CS₂)用于粘胶纤维的生产。工业上常用曝气手段去除CS₂,然而一些CS₂会继续留存在水中并进入污水处理的硝化单元,影响运行效果。研究发现,短期胁迫下10 mg/L CS₂即显著抑制硝化污泥活性,低浓度CS₂(10~40 mg/L)会促进硝化污泥代谢活性和抗氧化活性,高浓度(100~200 mg/L)则会产生抑制。在长期CS₂(20~200 mg/L)胁迫下硝化反应被完全抑制,长期运行过程中发现硝化污泥表面有白色浑浊物,经液相色谱与XPS分析发现该白色浑浊物为单质硫,推测可能发生了CS₂→COS→H₂S→S⁰→SO^2-₃→SO^2-₄的同化过程。在16S rRNA分析中也发现了参与硫氧化的Actinobacteria、Sinomonas丰度升高,也验证了这一推测。
- 二硫化碳 /
- 硝化作用 /
- 代谢活性 /
- 氧化应激 /
- 微生物群落
Abstract: About one million tons of CS₂ is used in the production of viscose fiber in China every year. CS₂ is often removed by aeration in industry. However, some CS₂ remains in the water and enters the nitrification unit of wastewater treatment, affecting operational performance. It was found that 10 mg/L CS₂ significantly inhibited nitrification sludge activity under short-term stress, low concentration of CS₂ (10 to 40 mg/L) could promote the metabolic activity and antioxidant activity of nitrification sludge, and high concentration of CS₂ (100 to 200 mg/L) could inhibit the activity. The nitrification reaction was completely inhibited under long-term CS₂ stress (20 to 200 mg/L), and white turbidity was found on the surface of nitrification sludge during long-term operation. The white turbidity was analyzed as simple sulfur by liquid chromatography and XPS, and the assimilation process of CS₂→COS→H₂S→S⁰→SO^2-₃→SO^2-₄ might occur. In 16S rRNA analysis, it was found that the abundance of Actinobacteria and Sinomonas involved in sulfur oxidation has increased, which also verified this hypothesis.
- carbon disulfide /
- nitrification /
- metabolism activity /
- oxidative stress /
- microbial community

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