好氧颗粒污泥强化脱氮及微生物群落的演变

赵勇; 郝桂珍; 徐利; 熊晓莹; 张暄茉; 王佳伟

doi:10.13205/j.hjgc.202407015

好氧颗粒污泥强化脱氮及微生物群落的演变

doi: 10.13205/j.hjgc.202407015

赵勇¹,
郝桂珍^2,3, ,,
徐利^2,3,
熊晓莹²,
张暄茉²,
王佳伟^2,3

1. 河套学院水利与土木工程系, 内蒙古巴彦淖尔 015000;
2. 河北建筑工程学院市政与环境工程系, 河北张家口 075000;
3. 河北省水质工程与水资源综合利用重点实验室, 河北张家口 075000

基金项目:

河北省高等学校科学技术研究项目(ZD2021316)

河北省研究生创新资助项目(CXZZSS2022062)

详细信息

作者简介:
赵勇(1995-),男,助教,主要研究方向为水处理及水资源技术。zhaoyong960424@163.com

通讯作者:
郝桂珍(1971-),女,教授,主要研究方向为水处理及污水资源化技术。609670233@qq.com

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出版历程
- 收稿日期: 2023-07-28
- 网络出版日期: 2024-12-02

ENHANCED NITROGEN REMOVAL BY AEROBIC GRANULAR SLUDGE AND EVOLUTION OF MICROBIAL COMMUNITY

1. Department of Water Conservancy and Civil Engineering, Hetao College, Bayannaoer 015000, China;
2. Department of Municipal and Environmental Engineering, Hebei University of Architecture, Zhangjiakou 075000, China;
3. Hebei Key Laboratory of Water Quality Engineering and Comprehensive Utilization of Water Resources, Zhangjiakou 075000, China

摘要

摘要: 为实现好氧颗粒污泥(AGS)系统高效的脱氮效果,在AGS系统前端设置厌氧段形成"厌氧/好氧" (A/O)运行模式来强化AGS系统的脱氮效果;同时研究整个试验过程中AGS的污泥特性和微生物群落结构的变化。结果表明:AGS以丝状菌作为骨架,活性污泥在水力剪切力的作用下不断地黏附在骨架上,进而形成AGS;其过程中胞外聚合物(EPS)组分中蛋白质含量显著增加,污泥表面Zeta电位值降低,提高了污泥间的凝聚性能,保证了AGS的形成和稳定性运行。污泥颗粒化阶段,unclassified_f__Sphingomonadaceae、unclassified_o__Saccharimonadales等具有疏水性且分泌EPS功能菌属的相对丰度上升,促进了污泥颗粒化。A/O运行模式的介入提高了AGS系统中与脱氮相关菌属的相对丰度,强化了AGS系统的脱氮性能;第110天低温环境的介入下,AGS系统依然保持稳定运行,最终TN去除率上升至78.2%,其主要归功于Zoogloea(由4.6%升至8.2%)、Thauera(由2.0%升至2.3%)、Rhodobacter(由2.3%升至2.7%)、norank_f__Actinomycetaceae(由1.8%升至3.7%)、Pseudoxanthomonas(由2.2%升至4.5%)等菌属的相对丰度上升。
- 好氧颗粒污泥 /
- 微生物群落 /
- 强化脱氮 /
- A/O模式
Abstract: To realize the efficient nitrogen removal of aerobic granular sludge (AGS), this study set an anaerobic section at the front end of the AGS system to form an anaerobic/aerobic (A/O) operation mode, to enhance the denitrification of the AGS system. At the same time, the changes in sludge characteristics and microbial community structure of AGS during the whole experiment were studied. The results showed that AGS was composed of filamentous bacteria as the skeleton, and the activated sludge continuously adhered to the skeleton under the action of hydraulic shear force to form AGS. During the process, the protein content in the extracellular polymeric substance (EPS) increased significantly, and the Zeta potential value of the sludge surface decreased, which improved the coagulation performance between the sludge and ensured the formation and stable operation of AGS. In the sludge granulation stage, the relative abundance of hydrophobic and EPS-secreting bacteria, such as unclassified_f__Sphingomonadaceae and unclassified_o__Saccharimonadales increased, which promoted sludge granulation. The intervention of A/O operation mode increased the relative abundance of nitrogen-related bacteria in the AGS system and enhanced the nitrogen removal performance of the AGS system. On the 110th day, the interventional AGS system in the low-temperature environment still maintained stable operation, and the final TN removal rate increased to 78.2%. The main reason was attributed to the increase in the relative abundance of Zoogloea (4.6%→8.2%), Thauera (2.0%→2.3%), Rhodobacter (2.3%→2.7%), norank_f__Actinomycetaceae (1.8%→3.7%), Pseudoxanthomonas (2.2%→4.5%), etc.
- aerobic granular sludge /
- microbial community /
- strengthen denitrification /
- A/O model

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