藻菌共生短程脱氮系统构建及污泥菌群结构分析

包美玲; 胡智泉; 张强; 洪慧; 邓俊; 裴云霞; 李炳堂

doi:10.13205/j.hjgc.202408005

藻菌共生短程脱氮系统构建及污泥菌群结构分析

doi: 10.13205/j.hjgc.202408005

包美玲^1,2,
胡智泉^2, ,,
张强¹,
洪慧¹,
邓俊¹,
裴云霞¹,
李炳堂²

1. 湖北省生态环境科学研究院(省生态环境工程评估中心), 武汉 430072;
2. 华中科技大学环境科学与工程学院, 武汉 430074

基金项目:

国家重点研发计划资助项目“活性污泥人工多细胞体系效能与安全性评估”(2019YFA0905504)

中央高校基本科研业务费专项资金资助项目“云南临沧市临翔区域生态环境综合治理与生态恢复”(2017KFKJFP002)

详细信息

作者简介:
包美玲(1994-),女,硕士,主要研究方向为养殖污水处理研究。2528036579@qq.com

通讯作者:
胡智泉(1971-),男,教授,主要研究方向为环境生物技术和生态能源研究。huzq@hust.edu.cn

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出版历程
- 收稿日期: 2023-04-10
- 网络出版日期: 2024-12-02

CONSTRUCTION OF A SHORTCUT NITROGEN REMOVAL SYSTEM FOR ALGAL-BACTERIAL SYMBIOSIS AND ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE IN SLUDGE

1. Hubei Provincial Academy of Eco-environment Sciences (Provincial Ecological Environment Engineering Assessment Center), Wuhan 430072, China;
2. School of Environmental Science & Engineering, Huazhong University of Science and Technology, Wuhan 430074, China

摘要

摘要: 低C/N高氨氮废水的传统生物脱氮方法存在曝气能耗大、外加碳源量高等不足。将富集氨氧化菌(AOB)的短程硝化功能污泥与小球藻联合构建藻菌共生短程脱氮系统,研究挂膜方式和光照强度等因素对系统脱氮除磷效能影响,并利用高通量测序技术手段分析微生物功能菌群结构,探究系统内的氮转化路径。结果表明:驯化污泥中氨氧化菌(AOB)相对丰度为19.31%,亚硝化单胞菌属(Nitrosomonas)在AOB中占比为94.41%,具有良好的短程硝化能力。藻菌共生系统实现了高效脱氮,在采用先接种污泥再接种微藻挂膜且设置光照强度为5000 lux时,其对模拟沼液的TN、TP去除效果最佳,去除率分别为93.22%、82.38%。藻菌共生系统稳定运行37 d后,生物膜中微生物丰富度提高,陶厄氏菌属(Thauera)相对丰度达56.42%,成为反硝化优势菌种,AOB相对丰度减少至5.65%,亚硝酸盐氧化菌(NOB)丰度仍极低,此时脱氮效率能够保持在90%以上。藻菌共生短程脱氮系统主要通过短程硝化反硝化(约88.46%)和生物同化作用(约6.79%)实现高效脱氮,比传统生物脱氮技术节省反硝化碳源约60.5%。
- 藻菌共生 /
- 系统构建 /
- 菌群结构 /
- 氮转化 /
- 短程硝化反硝化
Abstract: The conventional biological nitrogen removal of wastewater with low C/N ratio and high ammonia nitrogen has some deficiencies, such as high energy consumption of aeration, and high amount of added carbon source. In this study, a shortcut nitrogen removal system for algal-bacterial symbiosis was constructed by combining shortcut nitrification functional sludge enriched with ammonia-oxidizing bacteria (AOB) with Chlorella. The effects of the film coating method and light intensity on nitrogen and phosphorus removal in the system were studied. The high-throughput sequencing technology was used to analyze the microbial community structure, and to analyze and determine the nitrogen conversion pathway in the system. The results demonstrated that the relative abundance of AOB in acclimated sludge was 19.31%, of which the main strain was Nitrosomonas, accounting for 94.41% of total AOB. The shortcut nitrification ability of acclimated sludge was excellent. An efficient nitrogen removal was achieved in the algal-bacterial symbiotic system. When first inoculating sludge and then inoculating microalgae, and the light intensity was set to 5000 lux, the best removal efficiency of TN and TP from simulated biogas slurry in the system could be realized as high as 93.22% and 82.38%, respectively. After stable operation of the system for 37 days, the microbial richness of the algal-bacterial symbiotic biofilm increased, and the nitrogen removal efficiency was higher than 90%. In biofilm, Thauera, with an relative abundance of 56.42%, played a dominant role in denitrification, while the relative abundance of AOB was reduced to 5.65%, and the abundance of NOB was very low. The high efficiency of nitrogen removal was realized through the shortcut nitrification-denitrification process (about 88.46%) and biological assimilation (about 6.79%) in shortcut nitrogen removal system for algal-bacterial symbiosis. About 60.5% of the carbon source for denitrification was saved, compared with traditional biological denitrification technology.
- algal-bacterial symbiosis /
- system construction /
- microbial community structure /
- nitrogen conversion /
- shortcut nitrification-denitrification

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参考文献(28)

[1]	LI S N, CHU Y H, XIE P, et al. Insights into the microalgae-bacteria consortia treating swine wastewater: symbiotic mechanism and resistance genes analysis[J]. Bioresource Technology,2022,349:126892-126892.
[2]	汪晓军,陈永兴,陈振国.厌氧氨氧化及其处理低碳氮比氨氮废水的研究进展[J].工业水处理,2022,42(11):25-31.
[3]	袁强军,张宏星,陈芳媛.不同低碳氮比废水中好氧颗粒污泥的长期运行稳定性[J].环境科学,2020,41(10):4661-4668.
[4]	WANG C, QIAO S, ZHOU J T. Strategy of nitrate removal in anaerobic ammonia oxidation-dependent processes[J]. Chemosphere,2023,313.
[5]	LIU X G, KIM M, NAKHLA G, et al. Partial nitrification-reactor configurations, and operational conditions: performance analysis[J]. Journal of Environmental Chemical Engineering, 2020,8(4).
[6]	SEUNTJENS D, HAN M, KERCKHOF F M, et al. Pinpointing wastewater and process parameters controlling the AOB to NOB activity ratio in sewage treatment plants[J]. Water Research, 2018, 138: 37-46.
[7]	祝贵兵,彭永臻,郭建华.短程硝化反硝化生物脱氮技术[J].哈尔滨工业大学学报,2008(10):1552-1557.
[8]	REN S, WANG Z, JIANG H, et al. Stable nitritation of mature landfill leachate via in-situ selective inhibition by free nitrous acid[J]. Bioresource Technology,2021,340:125647-125647.
[9]	LI Q, XU Y F, LIANG C Z, Peng L, et al. Nitrogen removal by algal-bacterial consortium during mainstream wastewater treatment: transformation mechanisms and potential N₂O mitigation[J]. Water Research,2023,235:119890-119890.
[10]	WANG J L, DENG Y F, CHEN W, et al. Revealing the role of algae in algae enhanced bacteria consortia for municipal wastewater treatment: performance, characteristics, and microbial pathways[J]. Journal of Water Process Engineering,2023,53.
[11]	刘雨雪.藻菌生物膜处理水产养殖废水及其饲料化潜力研究[D]. 武汉:华中科技大学,2021.
[12]	陈文婷.固定化藻菌强化榨菜废水尾水的处理[D]. 重庆:重庆大学,2020.
[13]	WEI D, XUE X, YAN L, et al. Effect of influent ammonium concentration on the shift of full nitritation to partial nitrification in a sequencing batch reactor at ambient temperature[J]. Chemical Engineering Journal,2014,235:19-26.
[14]	CHEN C, SONG Y H, YUAN Y C. The Operating Characteristics of Partial Nitrification by Controlling pH and Alkalinity[J]. Water,2021,13(3):286-286.
[15]	VILLAVERDE S, GARCÍA E P A, FDZ P F. Influence of pH over nitrifying biofilm activity in submerged biofilters[J]. Water Research, 1997,31(5):1180-1186.
[16]	VADIVELU V M, YUAN Z G, FUX C, et al The inhibitory effects of free nitrous acid on the energy generation and growth processes of an enriched Nitrobacter culture[J]. Environmental Science & Technology,2006,40(14): 4442-4448.
[17]	LU S M, LIU X G, LIU C, et al. Influence of photoinhibition on nitrification by ammonia-oxidizing microorganisms in aquatic ecosystems[J]. Reviews in Environmental Science and Bio/Technology, 2020,19(3):531-542.
[18]	董昆,李奕燃,李晓强,等.游离氨对生物脱氮硝化过程细菌种群结构的影响[J].环境化学,2022,41(8):2742-2751.
[19]	SIRIPONG S, RITTMANN B E. Diversity study of nitrifying bacteria in full-scale municipal wastewater treatment plants[J].Water Research,2007,41(5):1110-1120.
[20]	CHEN H C, CHEN Z G, ZHOU S W, et al. Efficient partial nitritation performance of real printed circuit board tail wastewater by a zeolite biological fixed bed reactor[J]. Journal of Water Process Engineering,2023,53.
[21]	OLIVER T I, MOHAMED S Z, RANIA A H, et al Long-term aerobic granular sludge stability through anaerobic slow feeding, fixed feast-famine period ratio, and fixed SRT[J]. Journal of Environmental Chemical Engineering,2020,8(2).
[22]	BENNETT K, SADLER N C, WRIGHT A T, et al. Activity-Based Protein Profiling of Ammonia Monooxygenase in Nitrosomonas europaea[J]. Applied and Environmental Microbiology,2016,82(8):2270-2279.
[23]	WANG X X, FANG F, CHEN Y P, et al. N₂O micro-profiles in biofilm from a one-stage autotrophic nitrogen removal system by microelectrode[J]. Chemosphere,2017,175:482-489.
[24]	ABHISHEK D, PRADYUT K, SUNITA A. Two stage treatability and biokinetic study of dairy wastewater using bacterial consortium and microalgae[J]. Biocatalysis and Agricultural Biotechnology,2022,43.
[25]	ALCÁNTARA C, MUÑOZ R, NORVILL Z, et al. Nitrous oxide emissions from high rate algal ponds treating domestic wastewater[J]. Bioresource Technology,2015,177:110-117.
[26]	SHI F, LIU Z L, LI J L, et al. Alterations in microbial community during the remediation of a black-odorous stream by acclimated composite microorganisms[J].Journal of Environmental Sciences, 2022,118(8):181-193.
[27]	ZENG D W, MIAO J, WU G X, et al. Nitrogen removal, microbial community and electron transport in an integrated nitrification and denitrification system for ammonium-rich wastewater treatment[J].International Biodeterioration & Biodegradation,2018,133:202-209.
[28]	包美玲,胡智泉,吴滔,等. 藻菌共生光序批式生物膜反应器短程脱氮效能研究[J]. 水处理技术, 2021, 47 (6): 58-63 ,69.