高盐对异养耦合硫自养工艺影响及四氢甲基嘧啶羧酸缓解策略

李冉; 侯雅男; 李海波; 刘志华; 韩懿; 张道虹; 宋圆圆; 郭建博; 黄聪

doi:10.13205/j.hjgc.202412011

高盐对异养耦合硫自养工艺影响及四氢甲基嘧啶羧酸缓解策略

doi: 10.13205/j.hjgc.202412011

1. 天津城建大学水质科学与技术重点实验室, 天津 300000;
2. 中国科学院天津工业生物技术研究所, 天津 300308;
3. 台州学院, 浙江台州 318000

基金项目:

低C/N高盐废水生物强化脱氮技术开发(KHX2023-129)

天津市合成生物技术创新能力提升行动项目"有机废弃物生物制氢关键技术研发应用"(TSBICIP-CXRC-074)

国家自然科学基金项目(52000134)

详细信息

作者简介:
李冉(1999-),女,硕士研究生,主要研究方向为污水生物处理技术。lran1106@163.com

通讯作者:
侯雅男(1989-),女,副教授,主要研究方向为污水生物处理技术。houyn2013@163.com；李海波(1985-),男,副教授,主要研究方向为生物脱氮除磷技术。lhb19850725@163.com

侯雅男(1989-),女,副教授,主要研究方向为污水生物处理技术。houyn2013@163.com；李海波(1985-),男,副教授,主要研究方向为生物脱氮除磷技术。lhb19850725@163.com

计量
- 文章访问数: 44
- HTML全文浏览量: 13
- PDF下载量: 1
- 被引次数: 0
出版历程
- 收稿日期: 2024-03-07
- 网络出版日期: 2025-01-18

IMPACT OF HIGH SALT ON HETEROTROPHIC COUPLED SULFUR AUTOTROPHIC (HSAD) PROCESS AND ECTOINE MITIGATION STRATEGY

1. Tianjin Key Laboratory of Aquatic Science and Technology, Tianjin Chengjian University, Tianjin 300384, China;
2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;
3. Taizhou University, Taizhou 318000, China

摘要

摘要: 日益增加的含盐废水排放给异养耦合硫自养(HSAD)工艺带来了挑战。混养反硝化微生物对高盐胁迫的响应机制和可行的缓解策略需要进一步探索。研究发现,2%盐度下反硝化脱氮效率可提高到99.42%,当盐度升高到6%时,HSAD的反硝化性能显著下降,NO^-₃-N去除率从95.77%下降到38.01%,硫自养反硝化工艺(SAD)的贡献率继续高于异养反硝化(HD)。高盐度导致还原性辅酶Ⅰ含量和三磷酸腺苷水平在6%盐度时分别下降了10.74%、46.6%和56.28%。此外,反硝化酶(硝酸还原酶和亚硝酸还原酶)活性的降低以及反硝化功能菌数量的减少也是导致高盐胁迫下HSAD反硝化受到抑制的重要因素。值得注意的是,在盐度为6%的条件下添加250 mg/L的四氢甲基嘧啶羧酸可缓解盐度胁迫,并通过促进胞外聚合物物质的分泌和提高HSAD微生物的代谢活性来提高HSAD的反硝化效率。微生物群落分析表明,与不添加四氢甲基嘧啶羧酸的情况相比,HD 功能菌的丰度增加了3.99%,凸显了四氢甲基嘧啶羧酸对群落演替和稳定性的关键调节作用。该研究结果加深了对高盐废水对HSAD的抑制机制的认识,为硫基混养反硝化技术缓解盐胁迫提供了可行的技术方案。
- 高盐废水 /
- 异养耦合硫自养(HSAD)工艺 /
- 脱氮 /
- 微生物代谢 /
- 四氢甲基嘧啶羧酸 /
- 微生物群落
Abstract: Increasing saline wastewater discharge poses a challenge to denitrification by heterotrophic and sulfur autotrophic denitrification (HSAD). The response mechanisms and feasible mitigation strategies of heterotrophic denitrifying microorganisms to high salt stress need to be further explored. In this study, it was found that a salinity of 2% increased denitrification efficiency. However, the denitrification performance of HSAD significantly decreased when salinity increased to 6%, NO^-₃-N removal decreased from 95.77% to 38.01%, and the contribution of sulfur autotrophic denitrification (SAD) continued to be higher than that of heterotrophic denitrification (HD). High salinity stimulation resulted in nicotinamide adenine dinucleotide content and adenosine triphosphate levels decreasing by 10.74%, 46.6% and 56.28% respectively, at 6% salinity. In addition, the reduced activities of denitrifying enzymes (nitrate reductase and nitrite reductase) and the decrease in denitrifying functional bacteria were also important factors contributing to the inhibition of HSAD denitrification under high salt stress. Notably, adding 250 mg/L ectoine at 6% salinity alleviated the salinity stress, and enhanced the denitrification efficiency of HSAD by promoting the secretion of extracellular polymeric substances and increasing the metabolic activities of HSAD microorganisms. Microbial community analysis showed that the abundance of HD-functional bacteria increased by 3.99% compared to the case without ectoine, highlighting the key regulatory role of ectoine on community succession and stability. The results of this study deepened the understanding of the inhibition mechanism of HSAD by high-salt wastewater, and provided a feasible technical solution for sulfur-based mixotrophic denitrification technology to alleviate salt stress.
- high salinity wastewater /
- heterotrophic and sulfur autotrophic denitrification /
- nitrogen removal /
- microbial metabolism /
- ectoine /
- microbial community

HTML全文

参考文献(46)

[1]	路青, 刘宏雁, 郑博英, 等. 硫自养反硝化的污水脱氮技术研究[J]. 环境工程, 2023, 41(增刊2): 60-61,64.
[2]	WANG T, LI X, WANG H, et al. Sulfur autotrophic denitrification as an efficient nitrogen removals method for wastewater treatment towards lower organic requirement: a review[J]. Water Research, 2023, 245: 120569.
[3]	WANG H C, LIU Y, YANG Y M, et al. Element sulfur-based autotrophic denitrification constructed wetland as an efficient approach for nitrogen removal from low C/N wastewater[J]. Water Research, 2022, 226: 119258.
[4]	JIAN C Q, HAO Y R, LIU R T, et al. Mixotrophic denitrification process driven by lime sulfur and butanediol: denitrification performance and metagenomic analysis[J]. Science of the Total Environment, 2023, 903: 166654.
[5]	LI Y Y, LIU L, WANG H J. Mixotrophic denitrification for enhancing nitrogen removal of municipal tailwater: contribution of heterotrophic/sulfur autotrophic denitrification and bacterial community[J]. Science of the Total Environment, 2022, 814: 151940.
[6]	FU J J. Mitigating the detrimental effects of salt stress on anammox process: a comparison between glycine betaine and mannitol[J]. Science of the Total Environment, 2022,851:158221.
[7]	SHE Z L, WU L, WANG Q, et al. Salinity effect on simultaneous nitrification and denitrification, microbial characteristics in a hybrid sequencing batch biofilm reactor[J]. Bioprocess and Biosystems Engineering, 2018, 41(1): 65-75.
[8]	ZHU Z L, ZHOU H, ZOU J L, et al. Effect of salinity on the denitrification of the sulfur-based autotrophic denitrification system[J]. Water Cycle, 2023, 4: 95-103.
[9]	WANG R, ZHENG P, DING A Q, et al. Effects of inorganic salts on denitrifying granular sludge: the acute toxicity and working mechanisms[J]. Bioresource Technology, 2016, 204: 65-70.
[10]	MACÊDO W V, SAKAMOTO I K, AZEVEDO E B, et al. The effect of cations (Na⁺, Mg²⁺, and Ca²⁺) on the activity and structure of nitrifying and denitrifying bacterial communities[J]. Science of the Total Environment, 2019, 679: 279-287.
[11]	SUDMALIS D, MILLAH S K, GAGLIANO M C, et al. The potential of osmolytes and their precursors to alleviate osmotic stress of anaerobic granular sludge.[J]. Water Research, 2018, 147: 142-151.
[12]	OREN A. Thermodynamic limits to microbial life at high salt concentrations[J]. Environmental Microbiology, 2011, 13(8): 1908-1923.
[13]	XIA Y, JIANG X B, WANG Y X, et al. Enhanced anaerobic reduction of nitrobenzene at high salinity by betaine acting as osmoprotectant and regulator of metabolism[J]. Water Research, 2022, 223: 118982.
[14]	CYPLIK P, PIOTROWSKA-CYPLIK A, MARECIK R, et al. Biological denitrification of brine: the effect of compatible solutes on enzyme activities and fatty acid degradation[J]. Biodegradation, 2012, 23(5): 663-672.
[15]	SAHLE C J, SCHROER M A, JEFFRIES C M, et al. Hydration in aqueous solutions of ectoine and hydroxyectoine[J]. Physical Chemistry Chemical Physics, 2018, 20(44): 27917-27923.
[16]	Chinese National Standard. 2008. Chemical analysis methods of lithium fluoride—Part 8: Determination of sulphate content—Barium sulphate gravimetric method. GB/T 22660.8-2008. Beijing: Chinese National Standard.
[17]	KE G, DUANXIONG K, ZHANG X, et al. Synthesis of Quaternary Hydrotalcite-Carbon Nanotube Composite and Its Sulfate Adsorption Performance in Cement Paste[J]. Journal of Materials in Civil Engineering, 2023, 35(11): 04023400.
[18]	HE Y, GUO J B, SONG Y Y, et al. Acceleration mechanism of bioavailable Fe(Ⅲ) on Te(Ⅳ) bioreduction of Shewanella oneidensis MR-1: promotion of electron generation, electron transfer and energy level[J]. Journal of Hazardous Materials, 2021, 403: 123728.
[19]	SU C Y, ZHENG P, LIN X M, et al. Influence of amoxicillin after pre-treatment on the extracellular polymeric substances and microbial community of anaerobic granular sludge[J]. Bioresource Technology, 2019, 276: 81-90.
[20]	LIU Z, WU L, HAO Y, et al. Nitrogen removal performance and C, N, S transformation under dry-wet alterations of heterotrophic-autotrophic sequential bioretention system amended with waste reed and sulfur[J]. Journal of Cleaner Production, 2023, 426: 139167.
[21]	LI X, YUAN Y, DANG P Z, et al. Effect of salinity stress on nitrogen and sulfur removal performance of short-cut sulfur autotrophic denitrification and anammox coupling system[J]. Science of the Total Environment, 2023, 878: 162982.
[22]	YANG Z L, ZHU W Q, YU D S, et al. Enhanced carbon and nitrogen removal performance of simultaneous anammox and denitrification (SAD) with mannitol addition treating saline wastewater[J]. Journal of Chemical Technology & Biotechnology, 2019, 94(2): 377-388.
[23]	SHEN Z, XIE L, LYU C, et al. Effects of salinity on nitrite and elemental sulfur accumulation in a double short-cut sulfur autotrophic denitrification process[J]. Bioresource Technology, 2023, 369: 128432.
[24]	LIU J, CHU G, WANG Q, et al. Metagenomic analysis and nitrogen removal performance evaluation of activated sludge from a sequencing batch reactor under different salinities[J]. Journal of Environmental Management, 2022, 323: 116213.
[25]	SARVAJITH M, NANCHARAIAH Y V. Biological nutrient removal by halophilic aerobic granular sludge under hypersaline seawater conditions[J]. Bioresource Technology, 2020, 318: 124065.
[26]	LIU M, PENG Y Z, WANG S Y, et al. Enhancement of anammox activity by addition of compatible solutes at high salinity conditions[J]. Bioresource Technology, 2014, 167: 560-563.
[27]	CHEN B, QAISAR M, WANG K, et al. Response of simultaneous sulfide and nitrate removal process on acute toxicity of substrate concentration and salinity: single toxicity and combined toxicity[J]. Science of the Total Environment, 2022, 836: 155639.
[28]	SU X X, CHEN Y, WANG Y Y, et al. Impacts of chlorothalonil on denitrification and N₂O emission in riparian sediments: microbial metabolism mechanism[J]. Water Research, 2019, 148: 188-197.
[29]	XU N, LI H, GUO T, et al. Effect of ibuprofen on the sulfur autotrophic denitrification process and microbial toxic response mechanism[J]. Bioresource Technology, 2023, 384: 129261.
[30]	WAN R, WANG L, CHEN Y G, et al. Tetrabromobisphenol A (TBBPA) inhibits denitrification via regulating carbon metabolism to decrease electron donation and bacterial population[J]. Water Research, 2019, 162: 190-199.
[31]	ZHAO W, WANG Y Y, LIU S H, et al. Denitrification activities and N₂O production under salt stress with varying COD/N ratios and terminal electron acceptors[J]. Chemical Engineering Journal, 2013, 215/216: 252-260.
[32]	LEE I. Betaine is a positive regulator of mitochondrial respiration[J]. Biochemical and Biophysical Research Communications, 2015, 456(2): 621-625.
[33]	WEINISCH L, KIRCHNER I, GRIMM M, et al. Glycine betaine and ectoine are the major compatible solutes used by four different halophilic heterotrophic ciliates[J]. Microbial Ecology, 2019, 77(2): 317-331.
[34]	WANG Q, ZHAO Y X, LIU Y N, et al. Recovery mechanism of bio-promoters on Cr(Ⅵ) suppressed denitrification: toxicity remediation and enhanced electron transmission[J]. Water Research, 2024: 121230.
[35]	WANG J, LIU X L, JIANG X B, et al. Facilitated bio-mineralization of N, N-dimethylformamide in anoxic denitrification system: long-term performance and biological mechanism[J]. Water Research, 2020, 186: 116306.
[36]	HAN X M, WANG Z W, WANG X Y, et al. Microbial responses to membrane cleaning using sodium hypochlorite in membrane bioreactors: cell integrity, key enzymes and intracellular reactive oxygen species[J]. Water Research, 2016, 88: 293-300.
[37]	LIU S Q, WANG C, HOU J, et al. Effects of Ag NPs on denitrification in suspended sediments via inhibiting microbial electron behaviors[J]. Water Research, 2020, 171: 115436.
[38]	LIU Y, HAN Y, GUO J, et al. New insights of simultaneous partial nitritation, anammox and denitrification (SNAD) system to Zn(Ⅱ) exposure: focus on affecting the regulation of quorum sensing on extracellular electron transfer and microbial metabolism[J]. Bioresource Technology, 2022, 346: 126602.
[39]	NG H S, WAN P K, KONDO A, et al. Production and recovery of ectoine: a review of current state and future prospects[J]. Processes, 2023, 11(2): 339.
[40]	SUN Y N, ZENG Q Z, YANG Q, et al. Impacts of electric field coupled membrane bioreactor on phenol wastewater with high salinity: Performance, membrane fouling and eco-friendly strategy[J]. Journal of Water Process Engineering, 2024, 60: 105076.
[41]	WEN Q X, WANG Z F, LIU B Z, et al. Enrichment performance and salt tolerance of polyhydroxyalkanoates (PHAs) producing mixed cultures under different saline environments[J]. Environmental Research, 2024, 251: 118722.
[42]	WANG M F, HE J G, DONG X K, et al. Effect of salinity on performance and microbial community during granulation process in a sequencing batch reactor[J]. Water, 2023, 15(22): 3961.
[43]	LU C S, QIU J M, YANG Y, et al. A review of anaerobic granulation under high-salinity conditions: mechanisms, influencing factors and enhancement strategies[J]. Journal of Water Process Engineering, 2023, 55: 104227.
[44]	SUN Z Y, LI Y, LI M, et al. Steel pickling rinse wastewater treatment by two-stage MABR system: reactor performance, extracellular polymeric substances (EPS) and microbial community[J]. Chemosphere, 2022, 299: 134402.
[45]	XU N Y, LI H B, GUO T T, et al. Effect of ibuprofen on the sulfur autotrophic denitrification process and microbial toxic response mechanism[J]. Bioresource Technology, 2023, 384: 129261.
[46]	XIA Z G, WANG Q, SHE Z L, et al. Nitrogen removal pathway and dynamics of microbial community with the increase of salinity in simultaneous nitrification and denitrification process[J]. Science of the Total Environment, 2019, 697: 134047.