短程硝化反硝化IASBR工艺处理苯胺废水及菌群特征

韦凤琴; 孟海玲; 刘再亮; 乔亚凯; 曹祖鹏; 陶师玉

doi:10.13205/j.hjgc.202504016

短程硝化反硝化IASBR工艺处理苯胺废水及菌群特征

doi: 10.13205/j.hjgc.202504016

1. 安徽工业大学能源与环境学院,安徽马鞍山 243002;
2. 安徽工业大学生物膜法水质净化及利用技术教育部工程研究中心,安徽马鞍山 243032

基金项目:

生物膜法水质净化及利用技术教育部工程研究中心开放基金（BWPU2022KF03）；生物膜法水质净化及利用技术教育部工程研究中心自主研究开发课题（BWPU2022ZY01）；安徽工业大学省级大学生创新创业训练计划项目（S202110360396，S202210360401）

详细信息

作者简介:
韦凤琴（1999—），女，硕士研究生，主要研究方向为难降解废水处理。2693023138@qq.com

通讯作者:
孟海玲（1980—），女，副教授，主要研究方向为工业废水处理技术。menghlahut@163.com

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出版历程
- 收稿日期: 2023-04-25
- 录用日期: 2023-06-18
- 修回日期: 2023-05-21
- 刊出日期: 2025-04-01

Treatment of aniline wastewater by partial nitrificaion-denitrification IASBR process and microbial characteristics

1. School of Energy and Environment, Anhui University of Technology, Ma'anshan 243032, China;
2. Ministry of Education Biofilm Process Water Purification and Utilization Technology Engineering Research Center, Anhui University of Technology, Ma'anshan 243032, China

摘要

摘要: 在低溶解氧条件（0.5 mg/L左右）下，探讨间歇曝气序批式反应器（IASBR）处理不同浓度苯胺废水（5，20，30 mg/L）时有机物降解和短程硝化反硝化脱氮性能，并对微生物群落的演变规律进行分析。试验结果表明：在苯胺初始浓度为5~30 mg/L时，该IASBR工艺对COD和苯胺的处理效果良好，COD平均去除率超过80%；苯胺去除率在运行后期逐渐稳定，平均达到85%以上。苯胺浓度为5，20 mg/L时，反应器对NH₄⁺-N的去除率平均分别为98.01%和93.03%，总无机氮去除率平均分别为83.79%和79.74%，亚硝酸盐积累率平均分别为86.58%和95.57%，可有效实现短程硝化反硝化脱氮。而苯胺浓度为30 mg/L时，NH₄⁺-N和总无机氮去除率平均分别下降至50.57%和40.48%，这是由于苯胺浓度的提高抑制了硝化菌和反硝化菌的活性。高通量测序结果表明：加入苯胺后，污泥中微生物的多样性略有降低。C0（接种污泥）、C1（5 mg/L）和C2（20 mg/L）的优势门均为Bacteroidetes、Proteobacteria和Candidatus_Saccharibacteria。随着进水苯胺浓度的升高，可降解芳香族胺类化合物的部分菌属得到富集（如unclassified_Sphingobacteriales）；但参与氮代谢的菌属如unclassified_Xanthomonadaceae受到抑制。此外，3个污泥样品中AOB菌属较NOB均明显处于优势地位，是系统实现短程硝化的主要原因。基于PICRUSt软件的代谢预测结果和KEGG数据库的注释分析，预测出系统中微生物对苯胺的代谢主要为邻位代谢和间位代谢过程。
- 间歇曝气 /
- 短程硝化反硝化 /
- 苯胺 /
- 菌群 /
- 代谢途径
Abstract: The study investigated the performance of an intermittent aeration sequencing batch reactors （IASBR） in low dissolved oxygen conditions （approximately 0.5 mg/L）. Degradation of organic compounds and nitrogen removal through the partial nitrification and denitrification were studied for treating of wastewater with different initial aniline concentrations （5， 20 and 30 mg/L）. The evolution of microbial communities were also analyzed based on high-throughput sequencing data. The experimental results indicated that when aniline concentration was from 5 mg/L to 30 mg/L， the IASBRs demonstrated excellent performance in removal of COD and aniline. The average removal rate of COD exceeded 80%. The removal rate of aniline was 85% above on average when the reactors gradually stabilized in the later stages. When the concentrations of aniline were 5 mg/L and 20 mg/L， the average NH₄⁺-N removal rates were 98.01% and 93.03%， the average total inorganic nitrogen removal rates were 83.79% and 79.74%， and the average accumulation rates of nitrite were 86.58% and 95.57%， respectively. The performance of partial nitrification and denitrification could effectively achieve nitrogen removal. However， when the aniline concentration increased to 30 mg/L， the average removal rates of NH₄⁺-N and total inorganic nitrogen decreased to 50.57% and 40.48%， respectively， because higher concentrations of aniline inhibited the activity of nitrobacteria and denitrifying bacteria. High-throughput sequencing results revealed a slight decrease in microbial diversity of sludge after adding aniline. The dominant phyla in test group C0 （inoculated sludge）， C1 （5 mg/L）， and C2 （20 mg/L） were Bacteroidetes， Proteobacteria and Candidatus_Saccharibacteria. With increasing influent aniline concentration， certain bacterial genera capable of degrading aromatic amine compounds were enriched， such as unclassified_Sphingobacteriales. However， bacterial genera involved in nitrogen metabolism were inhibited， such as unclassified_Xanthomonadaceae. Additionally， AOB （ammonia oxidizing bacteria） had a significant advantage over NOB （nitrite oxidizing bacteria） in three sludge samples， indicating that AOB had stronger tolerance to aniline. It was the main reason for achieving partial nitrification denitrification in the system. Metabolic predictions based on PICRUSt software and annotation analysis using the KEGG database indicated that the microbial metabolism of aniline in the system mainly involved ortho-metabolism and meta-metabolism processes.
- intermittent aeration /
- partial nitrification-denitrification /
- aniline /
- microbial community /
- metabolic pathway

HTML全文

1 IASBR装置示意

1—进水桶 2—蠕动泵 3—曝气泵 4—转子流量计 5—曝气砂头 6—转子 7—微生物 8—温度传感器 9—出水桶

1. Schematic diagram of the IASBR device

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2 3个反应器对COD和苯胺的去除率

2. Removal rates of COD and aniline in the three reactors

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3 3个反应器的脱氮性能

3. The nitrogen removal performance of the three reactors

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4 IASBR处理焦化废水各污染物的去除效果

4. The removal effects of various pollutants in coking wastewater treated by IASBR

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5 C0、C1和C2在门和属水平上的微生物群落结构

5. Microbial community structures of C0， C1 and C2 at phyla level and genus level

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6 C1和C2中微生物代谢苯胺相关功能基因及序列数

6. Functional genes and sequence numbers related to microbial metabolism of aniline in C1 and C2

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7 微生物代谢苯胺途径预测

7. Prediction of aniline degradation pathway by microorganism

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1 微生物α多样性指数

1. Alpha diversity indices of the microbials

样品 OTUs Chao Ace Shannon Simpson

C0 198.0 227.06 218.55 3.24 0.09
C1 209.0 248.18 235.09 2.64 0.21
C2 204.0 230.40 227.78 3.02 0.13

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