<i>Raoultella planticola</i>/MoS<sub>2</sub>生物杂合体增强偶氮染料的脱色机理

温娴; 侯雅男; 李海波; 韩懿; 张道虹; 宋圆圆; 郭建博; 刘志华; 黄聪

doi:10.13205/j.hjgc.202505008

Raoultella planticola/MoS₂生物杂合体增强偶氮染料的脱色机理

doi: 10.13205/j.hjgc.202505008

1. 天津城建大学水质科学与技术重点实验室, 天津 300384;
2. 中国科学院天津工业生物技术研究所, 天津 300308;
3. 台州学院, 浙江台州 318000

基金项目:

国家自然科学基金项目（52000134）；天津市合成生物技术创新能力提升项目（TSBICIP-CXRC-074）

详细信息

作者简介:
温娴（1997—），女，硕士研究生，主要研究方向为污水生物处理技术。912713629@qq.com

通讯作者:
侯雅男（1989—），女，副教授，主要研究方向为污水生物处理技术。houyn2013@163.com；黄聪（1984—），男，副研究员，主要研究方向为工业废水处理技术。huangc@tib.cas.cn

侯雅男（1989—），女，副教授，主要研究方向为污水生物处理技术。houyn2013@163.com；黄聪（1984—），男，副研究员，主要研究方向为工业废水处理技术。huangc@tib.cas.cn

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出版历程
- 收稿日期: 2024-03-08
- 录用日期: 2024-07-12
- 修回日期: 2024-05-21
- 网络出版日期: 2025-09-11

Decolorization mechanism of azo dyes enhanced by Raoultella Planticola/MoS₂ biohybrids

1. Tianjin Key Laboratory of Aquatic Science and Technology, Tianjin Chengjian University, Tianjin 300384, China;
2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;
3. Taizhou University, Taizhou 318000, China

摘要

摘要: 传统偶氮染料的生物脱色通常受到低电子转移速率的限制，可以通过添加具有氧化还原特性的纳米颗粒来形成稳定的生物杂合体来克服该问题。研究构建了具有良好生物相容性的表面沉淀型R.planoultella/MoS₂生物杂合体。批量实验表明，杂合0.1~0.7 mmol/L的二硫化钼（MoS₂）纳米材料对甲基橙（MO）的生物脱色均有促进作用，其中R.planticola/MoS_{2（0.5 mmol/L）}脱色效果最快。研究发现，R.planticola/MoS₂提高了微生物代谢活性和电子传递能力，烟酰胺腺嘌呤二核苷酸（NADH）、三磷酸腺苷（ATP）和电子传递系统活性（ETSA）分别提高了92.08%、42.61%和85.89%。另外，R.planoultella/MoS₂生物杂合体增加了胞外聚合物（EPS）的含量，且促进表达了更多的氧化还原性物质和官能团。该研究结果为加速偶氮染料的生物降解提供了新策略，有利于加深对纳米材料与微生物相互作用的理解。
- R. planticola/MoS₂ /
- 甲基橙 /
- 电子传递 /
- 物质代谢
Abstract: The biological decolorization of traditional azo dyes is usually limited by low electron transfer rates， which can be addressed by the addition of nanoparticles with redox-active properties to form a stable biohybrid system. A surface-precipitated R. planticola/MoS₂ biohybrid with good biocompatibility was constructed. Batch experiments showed that biohybrids incorporating 0.1-0.7 mmol/L molybdic sulfide （MoS₂） promoted the biological decolorization of methyl orange （MO），with R. planticola/MoS_{2（0.5mmol/L）} exhibiting the fastest decolorization efficiency. The R. planticola/MoS₂ biohybrid improved microbial metabolic activity and electron transfer capacity， as evidenced by a respective increase of 92.08%， 42.61%， 85.89%， and 37.58% in the levels of nicotinamide adenine dinucleotide （NADH）， adenosine triphosphate （ATP）， and electron transfer system activity （ETSA）. In addition， the R. planticola/MoS₂ biohybrid increased the content of extracellular polymeric substances（EPS） and promoted the expression of redox-active substances and functional groups. This study provided a novel strategy for accelerating the biodegradation of azo dyes and deepened the understanding of the interactions between nanomaterials and microorganisms.
- R. planticola/MoS₂ /
- methyl orange /
- electron transfer /
- substance metabolism

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