不同外源添加剂对养殖粪污除氨效果的影响

罗妍; 张奎; 彭璧辉; 李松蓉; 马宏芳; 李季; 任莉; 张陇利; 蒋正波; 魏雨泉

doi:10.13205/j.hjgc.202505012

不同外源添加剂对养殖粪污除氨效果的影响

doi: 10.13205/j.hjgc.202505012

罗妍¹,
张奎¹,
彭璧辉¹,
李松蓉¹,
马宏芳¹,
李季^1,2,
任莉³,
张陇利³,
蒋正波¹,
魏雨泉^1,2, ,

1. 中国农业大学资源与环境学院, 北京 100193;
2. 中国农业大学有机循环研究院(苏州), 江苏苏州 215100;
3. 北京沃土天地生物科技股份有限公司, 北京 100193

基金项目:

国家自然科学基金项目（42307436）；中央高校基本科研业务费专项资金项目（2024TC003）。

详细信息

通讯作者:
魏雨泉（1991—），男，博士，副教授，主要研究方向为生态工程与有机废弃物处理。weiyq2019@cau.edu.cn

计量
- 文章访问数: 72
- HTML全文浏览量: 29
- PDF下载量: 0
- 被引次数: 0
出版历程
- 收稿日期: 2024-08-12
- 录用日期: 2024-11-13
- 修回日期: 2024-09-10
- 网络出版日期: 2025-09-11

Effect of different exogenous additives on ammonia removal efficiency of aquaculture manure

1. College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China;
2. Organic Recycling Research Institute (Suzhou) of China Agricultural University, Suzhou 215100, China;
3. Beijing VOTO Biotechnology Co. Ltd., Beijing 100193, China

摘要

摘要: 为缓解养殖场中粪污因发酵产生的恶臭氨气所造成的污染，利用选择培养基筛选出除氨菌株，并进行16S rDNA基因序列分析鉴定，分别向粪污中添加效果较好的除氨菌、植物提取物和物理吸附材料等不同外源添加剂，利用室内模拟培养探究其除氨效果，并对其经济效益进行评价，优选出最佳粪污除氨外源添加剂。结果表明：分离得到6株效果较好的除氨菌株，V1、V2、L1、L2为副伯克霍尔德氏菌（Paraburkholderia fungorum），L3、L4为罗尔斯通菌（Ralstonia insidiosa），且在24 h氨氮去除率均在60.0%以上，48 h氨氮去除率均在90.0%以上；在不同植物提取物——丝兰提取物、肉桂提取物、橡树叶提取物和柠檬提取物中，丝兰提取物对NH₃的去除效果最好，24 h氨氮去除率为12.9%，48 h氨氮去除率为14.8%；在不同物理吸附材料——生物炭、改性生物炭和硅藻土中，硅藻土对NH₃的去除效果最好，24 h氨氮去除率为26.0%，48 h氨氮去除率为22.8%；不同外源添加剂中除氨菌和植物提取物的成本较低，但除氨菌在24，48 h累计NH₃去除率比植物提取物分别高出47.0~48.0百分点和75.0%~80.0百分点。因此，对于养殖场粪污除臭尤其是NH₃减排控制，应首先选用除氨菌剂，植物提取物次之。该研究为低成本、高效快速除氨的外源添加剂选择提供了一定的理论支撑。
- 氨气减排 /
- 畜禽粪污 /
- 除臭微生物 /
- 植物提取物 /
- 吸附材料
Abstract: To alleviate the pollution caused by the foul-smelling ammonia gas produced by manure fermentation in aquaculture farms， ammonia removal strains were screened using selective culture media， and 16S rDNA gene sequence analysis and identification were carried out. Different exogenous substances such as ammonia removal bacteria， plant extracts， and physical adsorption materials with good effects were added separately. The ammonia removal effect was explored using an indoor simulation culture， and its economic benefits were evaluated to select the best exogenous additive for manure ammonia removal. The results showed that six strains with good ammonia removal effect were isolated. V1， V2， L1 and L2 were Paraburkholderia fungorum， while L3， L4 were Ralstonia insidiosa. The ammonia nitrogen removal rates were all above 60.0% within 24 hours and above 90.0% within 48 hours； among different plant extracts such as yucca extract， cinnamon extract， oak leaf extract， and lemon extract， yucca extract had the best removal effect on NH₃， with an ammonia nitrogen removal rate of 12.9% within 24 hours and 14.8% within 48 hours； among different physical adsorption materials such as biochar， modified biochar， and diatomaceous earth， diatomaceous earth had the best removal effect on NH₃， with an ammonia nitrogen removal rate of 26.0% within 24 hours and 22.8% within 48 hours； the costs of ammonia removal bacteria and plant extracts were low in different exogenous additives， but the cumulative NH₃ removal rate of ammonia removal bacteria was 47.0 to 48.0 percentage points and 75.0 to 80.0 percentage points higher than that of plant extracts within 24 hours and 48 hours， respectively. Therefore， for the deodorization of manure in aquaculture farms， especially for reducing ammonia emissions， ammonia removal agents should be selected first， followed by plant extracts. This study provides theoretical support for selecting low-cost， efficient， and rapid exogenous additives for ammonia removal.
- ammonia emission reduction /
- livestock manure /
- deodorizing microorganisms /
- plant extracts /
- adsorption materials

HTML全文

参考文献(33)

[1]	CAO T T，ZHENG Y H，DONG H M. Control of odor emissions from livestock farms:a review[J]. Environmental Research，2023，225:115545.
[2]	ZHANG J，XING L，CHENG G，et al. Diffusion model simulation of odor pollutants in livestock and poultry farms based on environmental monitoring of the internet of things[J]. EDP Sciences，2021，261:3018.
[3]	PICCARDO M T，GERETTO M，PULLIERO A，et al. Odor emissions:a public health concern for health risk perception[J]. Environmental Research，2022，204:112121.
[4]	WANG Y，XUE W，ZHU Z，et al. Mitigating ammonia emissions from typical broiler and layer manure management-a system analysis[J]. Waste Management，2019，93:23-33.
[5]	JHA R，BERROCOSO J F D. Dietary fiber and protein fermentation in the intestine of swine and their interactive effects on gut health and on the environment:a review[J]. Animal Feed Science and Technology，2016，212:18-26.
[6]	SUN D S，JIN X，SHI B L，et al. Effects of Yucca schidigera on gas mitigation in livestock production:a review[J]. Brazilian Archives of Biology and Technology，2017，60.
[7]	QIAN Y，SONG K H，ZHAO R M. Research advances in relationship between ammonia pollution and PM_2.5[J]. Environmental Engineering，2018，36（5）:84-88. 钱翌，宋开慧，赵荣敏. 氨气污染与PM_2.5的关系研究进展[J]. 环境工程，2018，36（5）:84-88.
[8]	MARTINEZ J，DABERT P，BARRINGTON S. Livestock waste treatment systems for environmental quality，food safety，and sustainability[J]. Bioresource Technology，2009，100（22）:5527-5536.
[9]	YU Z，GUO H，LAGUE C. Livestock odor dispersion modeling:a review[J]. Transactions of the Asabe，2010，53（4）:1231-1244.
[10]	RZEZNIK W，MIELCAREK B P. Odour emissions from livestock buildings[J]. Atmosphere，2022，13（2）:254.
[11]	WANG C，LIN C，SU W，et al. Effects of supplementing sow diets with fermented corn and soybean meal mixed feed during lactation on the performance of sows and progeny[J]. Journal of Animal Science，2018，96（1）:206-214.
[12]	RYU H W，CHO K S，LEE T H. Reduction of ammonia and volatile organic compounds from food waste-composting facilities using a novel anti-clogging biofilter system[J]. Bioresource Technology，2011，102（7）:4654-4660.
[13]	XIAO Y P，DAI B，SU Q，et al. The mechanism of odor generation in pig farms and the application of plant extracts in deodorization production[J]. Chinese Journal of Animal Science，2013，49（22）:60-64. 肖英平，代兵，苏琴，等. 猪场臭气的产生机理及植物提取物在除臭生产中的应用[J]. 中国畜牧杂志，2013，49（22）:60-64.
[14]	REN B M，ZHAO Y Q，LYCZKO N，et al. Current status and outlook of odor removal technologies in wastewater treatment plant[J]. Waste and Biomass Valorization，2019，10（6）:1443-1458.
[15]	PAN Y J，LIU F，MENG S，et al. Isolation and characterization of an aerobic denitrifying-heterotrophic bacterium[J]. Environmental Engineering，2016，34（1）:41-46. 潘玉瑾，刘芳，孟爽，等. 好氧反硝化菌P.chengduensis ZPQ2的筛选及其反硝化条件优化[J]. 环境工程，2016，34（1）:41-46.
[16]	CHEN J L，ZHANG M M，ZHANG S N，et al. Heterotrophic nitrification-aerobic denitrification characteristics of Alcaligenes faecalis WT14 and its potential application in ammonia-rich wastewater[J]. Environmental Engineering，2021，39（2）:27-32，40. 陈均利，张苗苗，张树楠，等. Alcaligenes faecalis WT14的异养硝化-好氧反硝化特性及对高氨废水处理潜力[J]. 环境工程，2021，39（2）:27-32，40.
[17]	XIE J，WANG Z F，WANG Y，et al. Manure combined with biochar reduces rhizosphere nitrification potential and amoA gene abundance of ammonia-oxidizing microorganisms in acid purple soil[J]. Applied Soil Ecology，2023，181:158-167.
[18]	SANTACRUZ R R A，CHIEN Y H. Yucca schidigera extract:a bioresource for the reduction of ammonia from mariculture[J]. Bioresource Technology，2010，101（14）:5652-5657.
[19]	KATSUNUMA Y，NAKAMURA Y，TAYADA A，et al. Effect of yucca schidigera extract and saponins on growth of bacteria isolated from animal intestinal tract[J]. Animal Science Journal，2000，2（71）:164-170.
[20]	MAZLOOMI F，JALALI M. Effects of vermiculite，nanoclay and zeolite on ammonium transport through saturated sandy loam soil:Column experiments and modeling approaches[J]. Catena，2019，176:170-180.
[21]	CHE J，XU M，YANG G J，et al. Granular sludge with diatomite carrier for ammonia removal from aquaculture wastewater[J]. Chinese Journal of Environmental Engineering，2014，8（12）:5318-5322. 车鉴，徐牧，阳桂菊，等. 硅藻土固定化颗粒污泥对海水养殖废水除氨性能[J]. 环境工程学报，2014，8（12）:5318-5322.
[22]	NGUYEN L H，NGUYEN X H，NGUYEN N D K，et al. H₂O₂ modified-hydrochar derived from paper waste sludge for enriched surface functional groups and promoted adsorption to ammonium[J]. Journal of the Taiwan Institute of Chemical Engineers，2021，126:119-133.
[23]	LIU J，JI J S，LEI M G，et al. Research and effect evaluation of ammonia deodorizer for pig farms[J]. Animals Breeding and Feed，2015（3）:1-7. 刘婕，籍景淑，雷明刚，等. 猪场氨气除臭剂的研究及效果评价[J]. 养殖与饲料，2015（3）:1-7.
[24]	HE Q，ZHANG Y Z，SHEN H X，et al. Adsorption of ammonium in septic tank effluent by modified corn stalk biochar[J]. Technology of Water Treatment，2022，48（12）:19-23.
[25]	ZHAO J J，GU X Y，CHE D L，et al. Removal of ammonia gas from fattening sheep feces by spraying different deodorants[J]. Journal of Domestic Animal Ecology，2022，43（1）:59-64. 赵娟娟，谷晓杨，车大璐，等. 喷洒不同除臭剂对育肥羊粪便氨气的去除规律研究[J]. 家畜生态学报，2022，43（1）:59-64.
[26]	YANG G. The control effect of microbial combined plant compound deodorizer on fecal odor gas[D]. Chongqing:Chongqing University，2020. 杨岗. 添加微生物联合植物复合除臭剂对粪便恶臭气体控制效果研究[D]. 重庆:重庆大学，2020.
[27]	Ye J Y，Fu Q. Screening of skatole-degrading bacteria and control of human fecal odor by compound bacteria[J]. Annals of Microbiology，2023，73，22.
[28]	Zhou S Z，Li Y J，Liao X D，et al. A low-cost deodorizing spray net device for the removal of ammonia emissions in livestock houses[J]. Journal of Cleaner Production，2021，318.
[29]	He L T，Wang D H，Zhu T L，et al. Pyrolysis recycling of pig manure biochar adsorption material for decreasing ammonia nitrogen in biogas slurry[J]. The Science of the total environment2023，881.
[30]	SENATORE V，ZARRA T，GALANG M G，et al. Full-scale odor abatement technologies in wastewater treatment plants（WWTPs）:a review[J]. Water，2021，13（24）:3503.
[31]	BOROWSKI S，MATUSIAK K，POWALOWSKI S，et al. A novel microbial-mineral preparation for the removal of offensive odors from poultry manure[J]. International Biodeterioration&； Biodegradation，2017，119:299-308.
[32]	BALTRENAS P，JANUSEVICIUS T，ZAGORSKIS A，et al. Removal of ammonia by biofilters with straight and wavy lamellar plates[J]. International Journal of Environmental Science and Technology，2021，18（5）:1181-1190.
[33]	WYSOCKA I，GEBICKI J，NAMIESNIK J. Technologies for deodorization of malodorous gases[J]. Environmental Science and Pollution Research，2019，26（10）:9409-9434.