贫营养复合菌剂的构建及其与载体联合修复微污染水源水的效果研究

曹凯; 雷宇; 彭宇鸿; 谢翼飞; 宋乐友; 邓粤; 高垣; 张丹

doi:10.13205/j.hjgc.202507014

贫营养复合菌剂的构建及其与载体联合修复微污染水源水的效果研究

doi: 10.13205/j.hjgc.202507014

曹凯¹,
雷宇^2,3,
彭宇鸿¹,
谢翼飞²,
宋乐友¹,
邓粤¹,
高垣¹,
张丹^2, ,

1. 成都市沱江流域投资发展集团有限公司, 成都 610041;
2. 中国科学院成都生物研究所, 成都 610200;
3. 中国科学院大学, 北京 100049

详细信息

作者简介:
曹凯（1985—），男，高级工程师，主要研究方向为流域水环境综合治理。53640257@qq.com

通讯作者:
张丹（1995—），女，博士，助理研究员，主要研究方向为合成微生物群落。zhangdan@cib.ac.cn

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出版历程
- 收稿日期: 2024-08-26
- 录用日期: 2024-10-16
- 修回日期: 2024-09-10
- 网络出版日期: 2025-09-11

Construction of oligotrophic compound microbial inoculants and their combined effect with functional carriers for remediation of micro-polluted source water

1. Chengdu Tuojiang River Investment and Development Group Co., Ltd., Chengdu 610041, China;
2. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610200, China;
3. University of Chinese Academy of Sciences, Beijing 100049, China

摘要

摘要: 微污染水源水污染修复与水质提升有利于保障农村地区饮用水安全。微生物修复具有投资少、绿色环保低碳等优点，但是微生物菌剂在低浓度污水中存在生长较慢、容易流失的问题，目前仍缺乏有效的解决方法。基于此，研究针对微污染水源水中低浓度有机物和氨氮的去除，定向筛选贫营养土著降解菌、构建贫营养复合菌剂、开发微生物富集载体以联合修复微污染水源水。从微污染水源水中筛选出20株贫营养土著微生物，通过组配构建了贫营养复合菌剂FY并深入研究菌剂特性。30 ℃条件下，FY菌剂对微污染水源水中COD去除率达50.96%，NH₃-N去除率达79.42%。开发的微生物富集载体由聚己内酯、沸石、陶粒、活性炭热熔共混挤压而成，具备营养缓释、吸附氮磷、表面多孔的特性。载体与FY菌剂投加量为0.1%时，批次换水条件下微污染水源水中NH₃-N去除率为93%，载体表面可形成生物膜，表明载体可以稳定持留FY菌剂并促进功能菌生长。该研究为微污染水源水的修复提供了技术支持，有助于保障农村地区饮用水安全。
- 贫营养复合菌剂 /
- 微生物富集载体 /
- 微污染水源水 /
- 饮用水安全
Abstract: The remediation and quality improvement of micro-polluted source water plays a crucial role in ensuring the safety of drinking water in rural areas. Microbial remediation offers several benefits， including low investment， eco-friendly practices， and minimal carbon emissions. However， microbial inoculants face challenges such as slow growth and susceptibility to loss in low-concentration sewage， presenting a significant obstacle to their effectiveness. As a result， there is a press need for innovative solutions to address these issues and optimize the performance of microbial remediation methods. Additionally， it is essential to develop strategies that can enhance the resilience and longevity of microbial inoculants in order to maximize their impact on water quality improvement. By addressing these challenges，the effectiveness of microbial remediation can be bolstered， ultimately improving the safety and reliability of drinking water in rural communities. Safeguarding the quality of drinking water is paramount， and leveraging microbial remediation techniques can significantly contribute to achieving this goal. To address the issue of micro-polluted source water， in this paper，the focus was on screening and developing oligotrophic indigenous microbial inoculants and creating microbial enrichment carriers to degrade low-concentration organic matter and ammonia nitrogen. Twenty strains of oligotrophic indigenous microorganisms were successfully screened from the micro-polluted source water. Subsequently， the oligotrophic compound microbial inoculant FY was constructed through combination， and its characteristics were further studied. The findings revealed that at 30℃， the removal efficiency of COD and ammonia nitrogen in micro-polluted water reached 50.96% and 79.42%， respectively. Additionally， a microbial enrichment carrier made of polycaprolactone， zeolite， ceramsite， and activated carbon was developed through hot melt blending and extrusion. This carrier demonstrated sustained nutrient release， adsorption of nitrogen and phosphorus， and a porous surface. When the dosage of the carrier and FY microbial inoculant was 0.1%， the removal efficiency of ammonia nitrogen in the micro-polluted source water reached an impressive 93% under batch water exchange. Furthermore， a biofilm was observed to form on the surface of the carrier， indicating its capacity to stably retain the FY microbial inoculant and promote the growth of functional bacteria. The study contributes technical support for the remediation of micro-polluted source water， ultimately ensuring the safety of drinking water in rural areas.
- oligotrophic compound microbial inoculants /
- microbial enrichment carrier /
- micro-polluted source water /
- drinking water safety

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参考文献(27)

[1]	LIU Y,LI X,QIAO X C,et al. Current situation and suggestions on risk prevention and control for micro pollution of water resources[J]. Environmental Pollution&Control,2021,43(9):1205-1208.刘琰,李雪,乔肖翠,等.中国水源微污染现状及风险防控建议[J].环境污染与防治,2021,43(9):1205-1208.
[2]	AN B M,XU C H,HUANG Y J. Nitrate pollution characteristics research of drinking water sources in rural areas[J]. Water Resources Development and Management,2020(2):52-55.安宝明,徐从海,黄永军.农村饮用水水源地硝酸盐污染特性研究[J].水资源开发与管理,2020(2):52-55.
[3]	HUANG C Y,LUO T,CHA Y,et al. Analysis of pollution problems and protection countermeasures of drinking water sources of "thousands of tons and ten thousand people" [J]. Resources Economization&Environmental Protection,2024(4):63-66.黄春燕,罗涛,查阳,等."千吨万人"饮用水水源地污染问题及保护对策分析[J].资源节约与环保,2024(4):63-66.
[4]	CHEN S C,XIA H S,ZHOU J H. Characteristics and treatment technology of micro polluted water source in rural water supply[J]. Environmental Science and Management,2016,41(6):82-85.陈师楚,夏宏生,周建华.农村供水微污染水源特征及处理技术研究[J].环境科学与管理,2016,41(6):82-85.
[5]	TU Z Y. Application of ecological concepts in the design of small and medium-sized river training projects[J]. Development Guide to Building Materials,2023,21(12):185-187.屠振宇.生态理念在中小河流治理工程设计中的应用[J].建材发展导向,2023,21(12):185-187.
[6]	WANG J,GAN Z M. Research of water pollution control and restoration technology of urban rivers[J]. Leather Manufacture and Environmental Technology,2022,3(23):102-105.王娟,甘祝名.城市河流水污染治理与修复技术研究[J].皮革制作与环保科技,2022,3(23):102-105.
[7]	XU R,CHEN X Y,FU X P,et al. The current situation and the development tendency of black and odorous water treatment by microorganism method[J]. Yunnan Metallurgy,2018,47(5):82-88.许瑞,陈心仪,付先萍,等.微生物法治理黑臭水体的现状和发展趋势[J].云南冶金,2018,47(5):82-88.
[8]	LIU X L,XU Y Y,SONG C,et al. Analysis of treatment technologies and measures for the urban black-stinking water body[J]. Chinese Journal of Environmental Engineering,2019,13(3):519-529.刘晓玲,徐瑶瑶,宋晨,等.城市黑臭水体治理技术及措施分析[J].环境工程学报,2019,13(3):519-529.
[9]	QIAN D,YU M,WU Y,et al. Application of high-efficiency microbial agents in water environment treatment[J]. Water Sciences and Engineering Technology,2020(1):71-73.钱丹,于淼,武轶,等.高效微生物菌剂在水环境治理中的应用[J].水科学与工程技术,2020(1):71-73.
[10]	YANG Z Z,LIU M D,CAO J G,et al. Eutrophication regulation and control by combination of microorganism agents and aquatic plants for urban ecological village landscape water[J]. Advanced Materials Research,2013,777:168-172.
[11]	WEN Y,ZHAO G Z,ZHOU C B,et al. Treatment of domestic sewage by a new microbial agent[J]. Chinese Journal of Environmental Engineering,2013,7(5):1729-1734.文娅,赵国柱,周传斌,等.一种新型微生物菌剂处理生活污水[J].环境工程学报,2013,7(5):1729-1734.
[12]	WANG L,XING H C. Effectiveness of microbial fungicides in restoring landscape water bodies in Zhao Yuan Park,Handan City[J]. Agricultural Technology Service,2020,37(11):112-114.王磊,邢浩春.微生物菌剂修复邯郸市赵苑公园景观水体的效果[J].农技服务,2020,37(11):112-114.
[13]	JIANG Y Y,ZHU S S,ZHANG MIAO,et al. Progress in research on microbial co-culturing[J]. Chinese Journal of Microecology,2017,29(2):239-244.姜滢滢,朱双双,章苗,等.微生物共培养研究进展[J].中国微生态学杂志,2017,29(2):239-244.
[14]	SU X,WANG S H,LIU P W,et al. Preparation of microbial composite carrier and nitration performance of Halomonas sp. NH2B encapsulation[J]. Environmental Science&Technology,2023,46(S1):1-10.苏鑫,王诗涵,刘佩武,等.微生物复合载体制备及包埋Halomonas sp.NH2B的硝化性能[J].环境科学与技术,2023,46(增刊):1-10.
[15]	LI L L,WANG Y J,FANG S P,et al. Bioremediation of petroleum hydrocarbon-contaminated soil by immobilized bacteria[J]. Environmental Science&Technology,2024,47(8):52-58.李玲玲,王曰杰,房师平,等.固定化菌剂对石油烃污染土壤的修复研究[J].环境科学与技术,2024,47(8):52-58.
[16]	DENG J X. Targeted screening and application of heterotrophic nitrifying bacteria and their ammonia oxidation mechanisms[D]. Wuxi:Jiangnan University,2022.邓敬轩.异养硝化菌的定向筛选、应用及其氨氧化机制研究[D].无锡:江南大学,2022.
[17]	FAN J L,PENG X,CHEN S F,al e t v. Construction and application of compound bacterial agent for efficient degradation of kitchen waste[J]. Agricultural Science Technology,2023,24(3):29-38.
[18]	CHENG L L. Study on carbon release from multicarbon source composite carrier and its enhanced microbial nitrogen removal process[D]. Zhengzhou:Zhengzhou University,2019.程璐璐.多碳源复合载体的释碳及其强化微生物脱氮过程研究[D].郑州:郑州大学,2019.
[19]	YAN Z C,JIAO L Z,TANG R X. Effect of Fe²⁺ on anaerobic ammonia oxidation denitrification in the presence of organic matter[J]. China Water&Wastewater,2023,39(23):68-74.严子春,焦陇珍,唐瑞祥.有机物存在下Fe²⁺对厌氧氨氧化脱氮性能的影响[J].中国给水排水,2023,39(23):68-74.
[20]	WANG Q Y,ZHANG T J,ZHANG L Y,et al. Effects of carbon to nitrogen ratio on anaerobic co-digestion by mixing human manure with straw in rural areas[J]. Environmental Science&Technology,2023,46(12):101-108.王秋媛,张铁坚,张立勇,等.碳氮比对人粪与秸秆协同厌氧发酵的影响[J].环境科学与技术,2023,46(12):101-108.
[21]	Dan T,Jing H,Shen T,et al. Performance of production of polyhydroxyalkanoates from food waste fermentation with Rhodopseudomonas palustris[J]. Bioresource Technology,2023,385:129165.
[22]	Yu P B,Du J,Chen J X. Study on screening and identification of Bacillus in the process of high-temperature aerobic composting and its application[J]. Ecological Science,2020,39(6):199-205.
[23]	LI K,YANG F. Application of environmental biotechnology in water pollution treatment[J]. Cleaning World,2024,40(3):133-135.李凯,杨帆.环境生物技术在水污染治理中的应用[J].清洗世界,2024,40(3):133-135.
[24]	WANG Z J,GUI X W,LI Z L. Compounding of efficient microbial agents for kitchen waste aerobic hydrolysis and its application as wastewater treatment additive[J]. Chinese Journal of Applied and Environmental Biology,2023,29(4):836-842.王正江,桂许维,李振轮.厨余好氧水解高效菌剂复配及污水处理应用[J].应用与环境生物学报,2023,29(4):836-842.
[25]	XIE J W,ZHENG Z W,PAN B W,et al. Study on denitrification characteristics of a nitrogen removal composite microbial agent[J]. Journal of Food Science and Biotechnology,2024,43(3):76-84.谢嘉炜,郑展望,潘碧文,等.一种脱氮复合微生物菌剂的反硝化特性[J].食品与生物技术学报,2024,43(3):76-84.
[26]	QIAO C S,WANG Y C,REN Y H. Selection and preparation of microbial agents for degrading excess sludge[J]. Chinese Journal of Environmental Engineering,2017,11(3):1842-1850.乔长晟,王羿超,任友花.筛选制备微生物菌剂降解剩余污泥[J].环境工程学报,2017,11(3):1842-1850.
[27]	SHEN Z,WANG J. Biological denitrification using cross-linked starch/PCL blends as solid carbon source and biofilm carrier[J]. Bioresource Technology,2011,102(19):8835-8838.