INFLUENCE OF PETROLEUM CONTAMINATION ON SOIL MICROBIAL COMMUNITY AND ISOLATION AND IDENTIFICATION OF OIL-DEGRADING BACTERIA
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摘要: 为研究石油污染对土壤中细菌群落结构及土壤理化性质的影响,分离筛选石油降解菌,从陕北宝塔、吴起、靖边和延长4个县区采集石油污染土壤和未受石油污染土壤,测量土壤中石油、有机质、硝态氮、铵态氮、速效磷、速效钾的含量以及pH;采用高通量测序技术对2种土壤中细菌群落结构进行比较分析;并以石油为唯一碳源,从石油污染土壤中筛选高效石油降解菌,并对所筛选的高效石油降解菌进行16S rDNA鉴定。陕北4个县区石油污染土壤中铵态氮、硝态氮、速效磷和速效钾含量分别降低了0.57,6.63,4.34,8.91 mg/kg,有机质含量增加了2~21倍。石油污染土壤中细菌群落的丰富度和多样性均降低,其中变形菌门(Proteobacteria)和绿弯菌门(Chloroflexi)为主要菌门,分枝杆菌属(Mycobacterium)为丰度最高菌属。以石油为唯一碳源,分离得到8株石油降解菌,其中菌株OS33和菌株OS62-1在5 d内的石油降解率分别为80.51%和81.60%,经鉴定OS33为迪茨氏菌(Dietzia sp.),OS62-1为红球菌(Rhodococcus sp.)。石油污染发生后,土壤中细菌群落的丰富度和多样性降低,筛选的8株石油降解菌中OS62-1石油降解率最高,研究结果进一步丰富了陕北地区石油降解菌菌种资源库。Abstract: This research intended to investigate the influence of oil contamination on bacterial community,soil physico-chemical properties,and to isolate and identify oil-degrading bacteria.In this work,the microbial community structure of oil-contaminated soil and control soil in Baota,Wuqi,Jingbian,and Yanchang counties in northern Shaanxi were analyzed based on high-throughput sequencing technology,and the oil content,organic matter,nitrate nitrogen,ammonium nitrogen,available phosphorus,available potassium and pH were measured.With petroleum as the sole carbon source,oil-degrading bacteria were screened from oil-contaminated soils and identified based on 16S rDNA gene sequence.The contents of ammonium nitrogen,nitrate nitrogen,available phosphorus,and available potassium decreased by 0.57,6.63,4.34,8.91 mg/kg,respectively,and the content organic matter increased by 2~21 times.The richness and diversity of microorganisms were reduced after being contaminated with crude oil.In the oil-contaminated soil,Proteobacteria and Chloroflexi were the dominant phylum,and Mycobacterium was the genus with the highest abundance.Using petroleum as the only carbon source,8 strains of oil-degrading bacteria were isolated,among them,the 5-day oil degradation rates of OS33 and OS62-1 strains reached 80.51% and 81.60%.Finally,OS33 was identified as Dietzia sp.,and OS62-1 was Rhodococcus sp.After being contaminated with crude oil,the soil microbial community richness and diversity were decreased.8 oil-degrading bacteria were isolated and the highest 5-day oil degradation rate was 81.60%.This study further enriched the oil-degrading bacteria resource of northern Shaanxi.
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Key words:
- petroleum pollution /
- soil /
- degrading /
- Rhodococcus sp. /
- Dietzia sp.
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[1] 史权,张亚和,徐春明,等.石油组分高分辨质谱分析进展与展望[J].中国科学:化学, 2014, 44(5):694-700. [2] ROLSHAUSEN G, PHILLIP D, BECKLES D M, et al. Do stressful conditions make adaptation difficult?Guppies in the oil-polluted environments of southern Trinidad[J]. Evolutionary Applications, 2015, 8(9):854-870. [3] NZILA A. Biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons under anaerobic conditions:overview of studies, proposed pathways and future perspectives[J]. Environmental Pollution, 2018, 239(AUG.):788-802. [4] SAMANTA S K, SINGH O V, JAIN R K. Polycyclic aromatic hydrocarbons:environmental pollution and bioremediation-Science Direct[J]. Trends in Biotechnology, 2002, 20(6):243-248. [5] BARRON M G. Ecological impacts of the deepwater horizon oil spill:implications for immunotoxicity[J]. Toxicologic Pathology, 2012, 40(2):315-320. [6] JOHNSTON J E, LIM E, ROH H. Impact of upstream oil extraction and environmental public health:a review of the evidence[J]. Science of the Total Environment, 2019, 657:157-189. [7] 代小丽,王硕,李佳斌,等.石油污染土壤原位生物修复强化技术研究进展[J].环境工程技术学报, 2020, 10(3):456-466. [8] HUANG L P, YE J Y, JIANG K M, et al. Oil contamination drives the transformation of soil microbial communities:Co-occurrence pattern, metabolic enzymes and culturable hydrocarbon-degrading bacteria[J]. Ecotoxicology and Environmental Safety, 2021, 225:112740. [9] MUKHERJEE S, JUOTTONEN H, SIIVONEN P, et al. Spatial patterns of microbial diversity and activity in an aged creosote-contaminated site[J]. The ISME Journal, 2014(8):2131-2142. [10] PATEL V, SHARMA A, LAL R, et al. Response and resilience of soil microbial communities inhabiting in edible oil stress/contamination from industrial estates[J]. BMC Microbiology, 2016, 16(1):50. [11] ABHISEK D, RATUL S, HANDIQUE P J. Mapping the bacterial community in digboi oil refinery, india by high-throughput sequencing approach[J]. Current Microbiology, 2018, 75:1441-1446. [12] ROY A, SAR P, SARKAR J, et al. Petroleum hydrocarbon rich oil refinery sludge of North-East India harbours anaerobic, fermentative, sulfate-reducing, syntrophic and methanogenic microbial populations[J]. BMC Microbiology, 2018, 18(1):151. [13] LIU J W, MENG Z, LIU X Y, et al. Microbial assembly, interaction, functioning, activity and diversification:a review derived from community compositional data[J]. Marine Life ence&Technology, 2019, 1(1):112-128. [14] 张宝宝,余天飞,艾加敏,等. 3株石油降解菌鉴定与降解特性研究[J].延安大学学报(自然科学版), 2021, 40(1):32-37. [15] 鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社, 1999. [16] ZORNOZA R, MATAIX-SOLERA J, GUERRERO C, et al. Evaluation of soil quality using multiple lineal regression based on physical, chemical and biochemical properties[J]. Science of the Total Environment, 2007, 378(1/2):233-237. [17] GAO Y C, WANG J N, GUO S H, et al. Effects of salinization and crude oil contamination on soil bacterial community structure in the Yellow River Delta region, China[J]. Applied Soil Ecology, 2015, 86:165-173. [18] LEE L H, LIN H J. Effects of an oil spill on benthic community production and respiration on subtropical intertidal sandflats[J]. Marine Pollution Bulletin, 2013, 73(1):291-299. [19] ZHOU Z C, LIU Y, PAN J, et al. Gammaproteobacteria mediating utilization of methyl-, sulfur-and petroleum organic compounds in deep ocean hydrothermal plumes[J]. The ISME Journal, 2020:3136-3148. [20] 蔡萍萍,宁卓,何泽,等.采油井场土壤微生物群落结构分布[J].环境科学, 2018, 39(7):3329-3338. [21] 王丽静.原油污染对土壤水分运动和黑麦草生长的影响[D].杨凌:西北农林科技大学, 2021. [22] 魏样.石油污染对土壤性状的影响及植物修复效应研究[D].杨凌:西北农林科技大学, 2019. [23] 祁迎春,王建,同延安,等.陕北石油污染土壤植物修复品种的筛选[J].生态科学, 2015, 34(1):148-153. [24] 王娣.降解菌接种生物强化对石油污染土壤的修复特性研究[D].西安:西安建筑科技大学,2020. [25] 陈锐,赵玲侠,孙晓宇,等.陕北石油污染区土壤细菌群落结构解析[J].环境污染与防治, 2017, 39(11):1181-1185. [26] 许殷瑞,吴蔓莉,王丽,等.陕北油田区石油污染土壤微生物种群变化及影响因素[J].中国环境科学,2021,41(9):4349-4359. [27] KING G M, KOSTKA J E, HAZEN T C, et al. Microbial responses to the deepwater horizon oil spill:from coastal wetlands to the deep sea[J]. Annual Review of Marine Science, 2014, 7(1):3136-3148. [28] COLEMAN N V, BUI N B, HOLMES A J. Solubie di-iro monooxygenase gene diversity in soil, sediments and ethene enrichments[J]. Environmental Microbiology, 2006, 8(7):1228-1239. [29] 吴慧君,宋权威,郑瑾,等.微生物降解石油烃的功能基因研究进展[J].微生物学通报, 2020, 47(10):3355-3368. [30] HU B, WANG M X, GENG S, et al. Metabolic exchange with non-alkane-consuming pseudomonas stutzeri SLG510A3-8 improves n-alkane biodegradation by the alkane degrader dietzia sp. Strain DQ12-45-1b[J]. Applied and Environmental Microbiology, 2020,86(8):e02931-19. [31] MOHAMED E S, AL YACOUB Z H, VEDAKUMAR J V. Biocatalytic desulfurization of thiophenic compounds and crude oil by newly isolated bacteria[J]. Frontiers in Microbiology, 2015, 6(112):112. [32] 乔悦,常世辉,李晓涵,等.一株高效原油降解菌的分离鉴定和降解特性研究[J].现代化工, 2021, 41(3):120-124,129.
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