Li Shufang, Zhang Xiaowen, Li Mi, Fu Changliang, Tang Dongshan, Peng Ying. ISOLATION AND IDENTIFICATION OF A DOMINANT BACTERIA STRAIN FROM AURANIUM MILL TAILING PILES AND ITS URANIUM-TOLERANT PERFORMANCE[J]. ENVIRONMENTAL ENGINEERING , 2015, 33(8): 87-91. doi: 10.13205/j.hjgc.201508020
Citation:
Li Shufang, Zhang Xiaowen, Li Mi, Fu Changliang, Tang Dongshan, Peng Ying. ISOLATION AND IDENTIFICATION OF A DOMINANT BACTERIA STRAIN FROM A
URANIUM MILL TAILING PILES AND ITS URANIUM-TOLERANT PERFORMANCE[J]. ENVIRONMENTAL ENGINEERING , 2015, 33(8): 87-91. doi: 10.13205/j.hjgc.201508020
Li Shufang, Zhang Xiaowen, Li Mi, Fu Changliang, Tang Dongshan, Peng Ying. ISOLATION AND IDENTIFICATION OF A DOMINANT BACTERIA STRAIN FROM AURANIUM MILL TAILING PILES AND ITS URANIUM-TOLERANT PERFORMANCE[J]. ENVIRONMENTAL ENGINEERING , 2015, 33(8): 87-91. doi: 10.13205/j.hjgc.201508020
Citation:
Li Shufang, Zhang Xiaowen, Li Mi, Fu Changliang, Tang Dongshan, Peng Ying. ISOLATION AND IDENTIFICATION OF A DOMINANT BACTERIA STRAIN FROM A
URANIUM MILL TAILING PILES AND ITS URANIUM-TOLERANT PERFORMANCE[J]. ENVIRONMENTAL ENGINEERING , 2015, 33(8): 87-91. doi: 10.13205/j.hjgc.201508020
A strain of dominant bacteria was isolated from a uranium mill tailing piles located in Shaoguan city，Guangdong
province，China． The bacteria was identified as Bacillus thuringiensis ( named Bt) on the basis of its colony morphology，
physiological and biochemical characteristics，as well as 16S rDNA sequence homology analysis． Its uranium tolerance and the
effect of different conditions ( temperature，pH value，NaCl concentration) on its growth were evaluated through batch
experiments in the presence of 5 mg /L U6 + liquid culture through analyzing the value of D600 nm ． The results of single factor
growth character experiments indicated that the optimum temperature，pH and salinity range for growth were 30 ℃，pH = 7. 0，
and 0. 5 ～ 50 g /L( NaCl) ，respectively． The strain could tolerate a uranium concentration up to 100 mg /L U6 + at 25%
incubation amount，30 ℃，initial pH 4 ～ 10，and it could preserve high growth activities in the salinity less than 8%． The
results indicated that Bt strain has high resistance to uranium and has potential in application for bioleaching of uranium and
bioremediation of uranium-polluted soil．
Login
Register
E-alert
DownLoad: