MECHANISM OF SYNTHESIZING PHB BY <i>CUPRIAVIDUS NECATOR</i> WITH WASTE COOKING OIL AS THECARBON SOURCE BASED ON METABOLOMICS ANALYSIS

WANG Pan; SUN Rong; CHENG Meng-meng; REN An-dong; REN Lian-hai

doi:10.13205/j.hjgc.202001024

Volume 38 Issue 1

Mar. 2020

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WANG Pan, SUN Rong, CHENG Meng-meng, REN An-dong, REN Lian-hai. MECHANISM OF SYNTHESIZING PHB BY CUPRIAVIDUS NECATOR WITH WASTE COOKING OIL AS THECARBON SOURCE BASED ON METABOLOMICS ANALYSIS[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(1): 151-156,161. doi: 10.13205/j.hjgc.202001024

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MECHANISM OF SYNTHESIZING PHB BY CUPRIAVIDUS NECATOR WITH WASTE COOKING OIL AS THECARBON SOURCE BASED ON METABOLOMICS ANALYSIS

doi: 10.13205/j.hjgc.202001024

School of Light Industry, Beijing Technology and Business University, Beijing 100048, China

Received Date: 2019-01-07

Abstract

Abstract

Metabolomics method based on gas chromatography-mass spectrometry (GC-MS) was used to analyze the intracellular metabolites of Cupriavidus necator to obtain potential metabolic biomarkers and reveal the mechanism of poly-3-hydroxybutyrate (PHB) accumulation. The metabolite levels of bacteria under different pH conditions such as 6, 7, 8 and 9 were investigated. The metabolites data was analyzed by partial least-squares discrimination analysis (PLS-DA), which presented distinct difference between the samples of four different conditions. As the pH changes, 11 substances showed significant differences, including ornithine, fumarate, β-D-glucose, glutaric acid, inositol, butyric acid, glycine, L-asparagine, d-glucose, L-threonine and valine. According to PLS-DA and metabolic pathway analysis, glycine, ornithine, threonine, sparagine and fumaric acid were potential metabolic biomarkers. These metabolites influenced PHB production through the metabolic pathways of TCA cycle pathway, amino acid anabolism and pyruvate synthesis degradation.
- waste cooking oil,
- Cupriavidus necator,
- poly-3-hydroxybutyrate(PHB),
- metabonomics

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References(23)

References

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