EFFECTS OF HYDROGEN AND NITROGEN ON ANAEROBIC METHANE DEGRADATION COURSE AND MICROBIAL DIVERSITY

In the process of landfill stabilization, to study the influencing factors of anaerobic methane oxidation process, the influence of gaseous substances (hydrogen, nitrogen) on the anaerobic methane oxidation process was investigated. On this basis, the effect of simulated gas on the anaerobic methane degradation process and microbial population structure were clarified. The results showed that there were cycle processes of methane oxidation, nitrogen fixation, nitrification and denitrification under atmospheric nitrogen, while the introduction of hydrogen had a certain impact on the degradation process of anaerobic methane. The methane concentration decreased firstly, then increased and then decreased, and the carbon dioxide concentration first increased then decreased, which was due to the reduction of carbon dioxide by hydrogen. After the long-term domestication of nitrogen and hydrogen, the structures of the soil microbial community changed significantly, and the amount of bacteria with nitrification, denitrification and nitrogen fixation function increased, and the Methylococcale appeared. The consumption of oxygen by aerobic bacteria (such as methane oxidizing bacteria and nitrifying bacteria) was conducive to the process of denitrification and anaerobic methane oxidation, which provided favorable condition for the occurrence of anaerobic methane oxidation. The results revealed the influence of gaseous substances on the anaerobic methane oxidation process during landfill stabilization.