TOXIC EFFECTS OF HEAVY METALS CONTAMINATED SITE ON MALE GONAD OF <i>BUFO RADDEI</i>

HUANG Tao; LIU Ju-mei; PENG Qin; YU Qiu; ZAN Xiao-hui; WANG Zhi-yong; CAO You-ming; SI Wan-tong; CHEN Hui-xia

doi:10.13205/j.hjgc.202006006

Volume 38 Issue 6

Aug. 2020

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2020 > 38(6): 35-39,74

HUANG Tao, LIU Ju-mei, PENG Qin, YU Qiu, ZAN Xiao-hui, WANG Zhi-yong, CAO You-ming, SI Wan-tong, CHEN Hui-xia. TOXIC EFFECTS OF HEAVY METALS CONTAMINATED SITE ON MALE GONAD OF BUFO RADDEI[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(6): 35-39,74. doi: 10.13205/j.hjgc.202006006

Citation:

HUANG Tao, LIU Ju-mei, PENG Qin, YU Qiu, ZAN Xiao-hui, WANG Zhi-yong, CAO You-ming, SI Wan-tong, CHEN Hui-xia. TOXIC EFFECTS OF HEAVY METALS CONTAMINATED SITE ON MALE GONAD OF BUFO RADDEI[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(6): 35-39,74. doi: 10.13205/j.hjgc.202006006

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TOXIC EFFECTS OF HEAVY METALS CONTAMINATED SITE ON MALE GONAD OF BUFO RADDEI

doi: 10.13205/j.hjgc.202006006

1. Chongqing Key Laboratory of Environmental Materials & Remediation Technologies, College of Chemistry and Environmental Engineering, Chongqing University of Arts and Sciences, Chongqing 402160, China;
2. CAS Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;
3. National Engineering Laboratory for Hydrometallurgical Cleaner Production Technology, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;
4. School of Life Science and Technology, Inner Mongolia University of Science & Technology, Baotou 014010, China

Received Date: 2020-04-02

Abstract

Abstract

In this study, Bufo Raddei was taken as the research object, and the polluted water wetland and the relatively pollution-free Yellow River Wetland Reserve on the south side of the tailing wetland in Baotou, were taken as the research sites. Through analysis of morphological indexes, testis organ coefficient, sperm dynamic parameters, sperm deformity rate, sex hormone, micro-nucleus rate, and DNA damage of different male B. raddei, the toxic effect of compound pollution of tailing wetland on male gonads of B. raddei was studied. The results showed that the condition factor (CF) and the testis organ coefficient of B. raddei in tailing wetland was significantly higher than that in Xiaobaihe wetland (P < 0.01). In addition, the whipping frequency of sperms in the tailing wetland was significantly improved, while the sperm motility was significantly decreased (P<0.05). The sperm deformity rate, erythrocyte micro-nucleus, and DNA damage were obviously higher than that in the Xiaobaihe wetland (P<0.05). The results showed that the compound pollution of tailing wetland resulted in decrease of the sperm quality and increase of the micro-nucleus rate and DNA damage, which influenced the toxicological effect of male gonads. The toxic stress of compound pollution was eased by increasing the condition factor and the coefficient of spermatozoa. The above results had positive effect on the population protection, and ecological balance maintenance of amphibians in the compound pollution site of heavy metals.
- compound pollution,
- condition factor,
- sperm quality,
- micronucleus rate,
- DNA damage

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