SCREENING OF COLD-ADAPTED PETROLEUM DEGRADING BACTERIA AND CHARACTERIZING MICROFLORA FOR PETROLEUM DEGRADATION

JIA Ling-hui; ZHENG Shi-hao; SUN Li-hui; BAO Yong-ming

doi:10.13205/j.hjgc.202006041

Volume 38 Issue 6

Aug. 2020

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2020 > 38(6): 252-258

JIA Ling-hui, ZHENG Shi-hao, SUN Li-hui, BAO Yong-ming. SCREENING OF COLD-ADAPTED PETROLEUM DEGRADING BACTERIA AND CHARACTERIZING MICROFLORA FOR PETROLEUM DEGRADATION[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(6): 252-258. doi: 10.13205/j.hjgc.202006041

Citation:

JIA Ling-hui, ZHENG Shi-hao, SUN Li-hui, BAO Yong-ming. SCREENING OF COLD-ADAPTED PETROLEUM DEGRADING BACTERIA AND CHARACTERIZING MICROFLORA FOR PETROLEUM DEGRADATION[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(6): 252-258. doi: 10.13205/j.hjgc.202006041

Citation:

JIA Ling-hui, ZHENG Shi-hao, SUN Li-hui, BAO Yong-ming. SCREENING OF COLD-ADAPTED PETROLEUM DEGRADING BACTERIA AND CHARACTERIZING MICROFLORA FOR PETROLEUM DEGRADATION[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(6): 252-258. doi: 10.13205/j.hjgc.202006041

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SCREENING OF COLD-ADAPTED PETROLEUM DEGRADING BACTERIA AND CHARACTERIZING MICROFLORA FOR PETROLEUM DEGRADATION

doi: 10.13205/j.hjgc.202006041

1. School of Food and Environment Science and Technology, Dalian University of Technology, Panjin 124221, China;
2. School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China

Received Date: 2019-06-11

Abstract

Abstract

The performance of in-situ bio-remediation of petroleum-contaminated soils has been always unsatisfactory, because of complex composition of crude oil and its strong biological toxicity, as well as low microbial activity at low soil temperature. In this paper, 7 cold-adapted oil-degrading bacteria strains were screened and identified from oil polluted soils. Based on the degrading characteristics of the 7 bacteria strains for petroleum composition, 6 cold-adapted oil-degrading microfloras were prepared. The CO₂ production and O₂ consumption of 6 microfloras for degrading crude oil in 5 L fermenters were monitored online with a gas analyzer. The most efficient microflora, consisted of Arthrobacter sp. JLH 001, Acinetobacter baumannii JLH 002, Pseudomonas fragi JLH 003 and Arthrobacter sp. JLH 006, showed maximal O₂ consumption and CO₂ production capacity after about 48 h, and completely degraded crude oil (with content of 1%) after about 72 h at 15 ℃, and the degradation was significantly accelerated at 25 ℃. These results suggested that efficient in-situ bio-remediation of petroleum-contaminated soil could be realized by adding cold-adapted oil-degrading microfloras.
- petroleum contaminated soil,
- cold-adapted bacteria,
- petroleum degrading bacteria,
- bio-degradation,
- bioremediation

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