Source Journal of CSCD
Source Journal for Chinese Scientific and Technical Papers
Core Journal of RCCSE
Included in JST China
Volume 38 Issue 8
Nov.  2020
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ZHENG Jin, WANG Xin-yu, LI Jie, SONG Quan-wei, LI Hong-li, WANG Xiao-ling, TIAN Pei-ting. BIOREMEDIATION OF CRUDE OIL IN CONTAMINATED SOIL BY MICROORGANISMS IMMOBILIZED WITH HUMIC ACID-MODIFIED BIOFUEL ASH[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(8): 34-40. doi: 10.13205/j.hjgc.202008006
Citation: ZHENG Jin, WANG Xin-yu, LI Jie, SONG Quan-wei, LI Hong-li, WANG Xiao-ling, TIAN Pei-ting. BIOREMEDIATION OF CRUDE OIL IN CONTAMINATED SOIL BY MICROORGANISMS IMMOBILIZED WITH HUMIC ACID-MODIFIED BIOFUEL ASH[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(8): 34-40. doi: 10.13205/j.hjgc.202008006

BIOREMEDIATION OF CRUDE OIL IN CONTAMINATED SOIL BY MICROORGANISMS IMMOBILIZED WITH HUMIC ACID-MODIFIED BIOFUEL ASH

doi: 10.13205/j.hjgc.202008006
  • Received Date: 2020-04-27
  • In this study, humic acid-modified biofuel ash was used as the carrier to immobilize the petroleum degrading bacteria for remediating the crude oil contaminated soil, and the best modification conditions of biofuel ash and the remediation effect of immobilized bacteria were investigated. The results showed that the optimum conditions of modified biofuel ash were: 10~40 mesh for the particle size, 1∶1 for the liquid ratio and 4 h for the modification time. After modification, the biofuel ash had rich pore-like structures and rough surface, which were favorable to the adsorption of bacteria and the immobilized number reached 1.5×109 CFU/g. After 60 days’ remediation, the removal efficiency of total petroleum hydrocarbon was 51.9%, which was higher than free bacteria by 25.0%, and the removal efficiency of long-chain n-alkanes, aromatic hydrocarbons and colloids was improved by 9.6%, 31.7% and 37.5%, respectively. The application of immobilized biofuel ash protected and supported the petroleum degrading bacteria, enhanced the basal respiration rate and the soil enzyme activity, thus achieved highly efficient degradation of petroleum hydrocarbon in soil. Therefore, humic acid-modified biofuel ash is a promising immobilization material in the field of petroleum contaminated soil remediation.
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