INFLUENCING FACTORS ANALYSIS OF LIPID PRODUCTION BY <i>RHODOSPORIDIUM TORULOIDES</i> USING CRUDE GLYCEROL, A BY-PRODUCT FROM BIODIESEL PREPARATION FROM WASTE COOKING OIL

GAO Zhen; MA Xiao-yu; GAO Ming; WANG Xiao-na; ZHANG Min; WU Chuan-fu; WANG Qun-hui

doi:10.13205/j.hjgc.202008009

Volume 38 Issue 8

Nov. 2020

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2020 > 38(8): 52-57

GAO Zhen, MA Xiao-yu, GAO Ming, WANG Xiao-na, ZHANG Min, WU Chuan-fu, WANG Qun-hui. INFLUENCING FACTORS ANALYSIS OF LIPID PRODUCTION BY RHODOSPORIDIUM TORULOIDES USING CRUDE GLYCEROL, A BY-PRODUCT FROM BIODIESEL PREPARATION FROM WASTE COOKING OIL[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(8): 52-57. doi: 10.13205/j.hjgc.202008009

Citation:

GAO Zhen, MA Xiao-yu, GAO Ming, WANG Xiao-na, ZHANG Min, WU Chuan-fu, WANG Qun-hui. INFLUENCING FACTORS ANALYSIS OF LIPID PRODUCTION BY RHODOSPORIDIUM TORULOIDES USING CRUDE GLYCEROL, A BY-PRODUCT FROM BIODIESEL PREPARATION FROM WASTE COOKING OIL[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(8): 52-57. doi: 10.13205/j.hjgc.202008009

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INFLUENCING FACTORS ANALYSIS OF LIPID PRODUCTION BY RHODOSPORIDIUM TORULOIDES USING CRUDE GLYCEROL, A BY-PRODUCT FROM BIODIESEL PREPARATION FROM WASTE COOKING OIL

doi: 10.13205/j.hjgc.202008009

1. School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China;
2. Department of Bioscience and Biotechnology, Kyushu University, Fukuoka 8190395, Japan

Received Date: 2020-03-02

Abstract

Abstract

In this study, crude glycerol, a by-product in the process of biodiesel preparation from waste cooking oil, was used as carbon source to produce microbial lipid by Rhodosporidium toruloides. Through the optimization, the optimized fermentation conditions were determined as follows: seed resuscitation time of 24 h, enrichment time of 10 h, inoculation rate of 6%, initial crude glycerin concentration of 60 g/L, ratio of carbon to nitrogen of 60, organic nitrogen ratio to total nitrogen of 75%, fermentation volume of 100 mL, fermentation temperature of 30 ℃ and fermentation time of 6 d. Under the optimal condition, lipid production and lipid yield rate reached 10.56 g/L and 0.229, respectively. Compared with inorganic ammonium sulfate, the addition of organic peptone significantly increased the lipid content and lipid production. The performance of biodiesel was estimated by measuring the fatty acid composition of lipid and using Hoekman equation, which met all of the standards of China, the United States and the European Union. This study can not only provide a new strategy for the reutilization of crude glycerol, but also provide a economical raw material for the production of biodiesel.
- microbial lipid,
- Rhadosporidium toruloides,
- crude glycerol,
- influence factors,
- aerobic fermentation

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References

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