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Volume 38 Issue 9
Nov.  2020
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Article Navigation >  ENVIRONMENTAL ENGINEERING  > 2020  >  38(9): 236-240,184
HAN Jianjun, CHAI Lujun, WANG Guojin, ZHANG Yu, QIN Kangjia, ZHOU Man, LIANG Xuejie, HAO Junpeng, WANG Hui. ISOLATION AND IDENTIFICATION OF A NEW SULFATE-REDUCING BACTERIUM AND ITS IN SITU REMEDIATION EFFECT OF HEXAVALENT CHROMIUM-CONTAMINATED SOIL[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(2): 192-198. doi: 10.13205/j.hjgc.202402023
Citation: LU Hong-sheng, GAO Yu-ting, ZHANG Xue, SUN Pei-ming, QIU Meng-meng. REMOVAL OF LEAD(Pb2+)FROM SOIL WITH MICROBIAL FUEL CELLS TECHNOLOGY[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(9): 236-240,184. doi: 10.13205/j.hjgc.202009038
shu

REMOVAL OF LEAD(Pb2+)FROM SOIL WITH MICROBIAL FUEL CELLS TECHNOLOGY

doi: 10.13205/j.hjgc.202009038

  • School of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266590, China

  • Received Date: 2019-07-22
    Abstract
  • In order to investigate the removal capability of heavy metals from soil by microbial fuel cells, we measure the electrical performance of a double-chamber microbial fuel cell (MFCs), and salt bridge was used as the proton channel in MFCs. The concentration of lead(Pb2+) was determined by flame atomic absorption spectrophotometry. The enrichment of Pb on electrode plate was observed by scanning electron microscope and analyzed by energy dispersive spectroscopy. The systematic taxonomic position of the bacteria isolated from the anolyte was analyzed by isolation, purification, 16S rDNA sequencing, and the establishment of the system tree. The results showed that after 10 days of MFCs operation, the removal of lead (Pb2+) from soil was as high as 64.40%, and the maximum voltage was 69.63 mV. The systematic taxonomic position analysis of the bacteria isolated from the anolyte showed that the two bacteria had the highest homology of 100% with Stenotrophomonas maltophilia strain LH15 (KM893074) and Pseudomonas sp. putida strain (MF996382) respectively. Based on the above results, the two strains were defined into Stentrophomonas and Pseudomonas by genus, and named SKD-GYT-1 (LC479453) and SKD-GYT-2 (LC479454) respectively, and their power generation capacity remained to be explored. Soil microbial fuel cells have a high capacity of generating electricity and obvious effect on the removal of lead from soil.
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