SHUTTLE CHARACTERISTICS OF LNA AND HNA BACTERIA DURING DENITRIFICATION PROCESS

ZHANG Shuang-shuang; LI Zhi-hua; BEI Yuan; YANG Cheng-jian

doi:10.13205/j.hjgc.202011013

Volume 38 Issue 11

Apr. 2021

Turn off MathJax

Article Contents

Article Navigation > ENVIRONMENTAL ENGINEERING > 2020 > 38(11): 78-84

ZHANG Shuang-shuang, LI Zhi-hua, BEI Yuan, YANG Cheng-jian. SHUTTLE CHARACTERISTICS OF LNA AND HNA BACTERIA DURING DENITRIFICATION PROCESS[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(11): 78-84. doi: 10.13205/j.hjgc.202011013

Citation:

ZHANG Shuang-shuang, LI Zhi-hua, BEI Yuan, YANG Cheng-jian. SHUTTLE CHARACTERISTICS OF LNA AND HNA BACTERIA DURING DENITRIFICATION PROCESS[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(11): 78-84. doi: 10.13205/j.hjgc.202011013

Citation:

PDF( 1610 KB)

SHUTTLE CHARACTERISTICS OF LNA AND HNA BACTERIA DURING DENITRIFICATION PROCESS

doi: 10.13205/j.hjgc.202011013

1. Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China;
2. Shanxi Key Laboratory of Environmental Engineering, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China

Received Date: 2020-02-13
Available Online: 2021-04-23
Publish Date: 2021-04-23

Abstract

Abstract

Low nucleic acid (LNA) and high nucleic acid (HNA) bacteria show different characteristics under different environmental conditions, and the effect of denitrification process on bacteria is still unclear. The variations of free-swimming LNA and HNA bacteria under different denitrification conditions and microbial community were thus investigated in this study, it was found that free-swimming bacteria increased rapidly during the denitrification process, and greater the denitrification rate resulted in a higher increase of free-swimming bacteria. It was evidenced that LNA bacteria moved faster than HNA bacteria at the beginning of the denitrification reaction. HNA bacteria showed a rapid increase, either the denitrification reached a certain level, or the floc structure loose or break, indicating that LNA bacteria was mainly on the surface of flocs and functioned as glue, or filling materials of the pores of flocs. HNA bacteria functioned as a backbone of flocs. When starch was used as the carbon source for denitrification, free-swimming bacteria were reduced due to the bridge-capture of starch. However the denitrification of sodium acetate had a more significant effect on free-swimming bacteria than the bridge-capture of starch. In addition, HNA bacteria had higher abundance and diversity than LNA bacteria, and was found out as the main functional bacteria. LNA bacteria can respond faster to denitrification and be used as a signal for denitrification initiation.
- low nucleic acid bacteria (LNA),
- high nucleic acid bacteria (HNA),
- free-swimming bacteria,
- activated sludge,
- denitrification,
- floc structure

FullText(HTML)

References(30)

References

VILA C M, GASOL J M, SHARMA S, et al. Community analysis of high-and low-nucleic acid-containing bacteria in NW Mediterranean coastal waters using 16S rDNA pyrosequencing[J]. Environmental Microbiology, 2012, 14(6):1390-1402.

WANG Y, HAMMES F, BOON N, et al. Isolation and characterization of low nucleic acid (LNA)-conetnt bacteria[J]. The ISME Journal, 2009, 3(8):889-902.

SONG Y H, WANG Y F, MAO G N, et al. Impact of planktonic low nucleic acid-content bacteria to bacterial community structure and associated ecological functions in a shallow lake[J]. Science of the Total Environment, 2019, 658:868-878.

LACOSTE É, PIOT A, ARCHAMBAULT P, et al. Bioturbation activity of three macrofaunal species and the presence of meiofauna affect the abundance and composition of benthic bacterial communities[J]. Marine Environmental Research, 2018, 136:62-70.

LONGNECKER K, SHERR B, SHERR E. Variation in cell-specific rates of leucine and thymidine incorporation by marine bacteria with high and with low nucleic acid content of the Oregon coast[J]. Aquatic Microbial Ecology, 2006, 43(2):113-125.

RAMSEIER M K, GUNTEN U, FREIHOFER P, et al. Kinetics of membrane damage to high (HNA) and low (LNA) nucleic acid bacterial clusters in drinking water by ozone, chlorine, chlorine dioxide, monochloramine, ferrate (Ⅵ), and permanganate[J]. Water Research, 2011, 45(3):1490-1500.

MAO G N, SONG Y H, BARTLAM M, et al. Long-term effects of residual chlorine on Pseudomonas aeruginosa in simulated drinking water fed with low AOC medium[J]. Frontiers in Microbiology, 2018, 9:879.

PAN Z L, ZHOU J, LIN Z Y, et al. Effects of COD/TN ratio on nitrogen removal efficiency, microbial community for high saline wastewater treatment based on heterotrophic nitrification-aerobic denitrification process[J]. Bioresource Technology, 2020, 301:122726.

HOLMAN J, WAREHAM D. COD, ammonia and dissolved oxygen time profiles in the simultaneous nitrification/denitrification process[J]. Biochemical Engineering Journal, 2005, 22(2):125-133.

FOGLAR L, BRIŠKI F. Wastewater denitrification process-the influence of methanol and kinetic analysis[J]. Process Biochemistry, 2003, 39(1):95-103.

CARLA C, ANNALISA O H, IBRAHIM E S, et al. Implication of using different carbon sources for denitrification in wastewater treatments[J]. Water Environment Research, 2009, 81(8):788-799.

郑志佳, 吴迪, 张晶晶,等. 两级后置纯膜MBBR的反硝化性能研究[J]. 环境工程, 2019, 37(9):68-73.

XU Z S, DAI X H, CHAI X L. Effect of different carbon sources on denitrification performance, microbial community structure and denitrification genes[J]. Science of the Total Environment, 2018, 634:195-204.

FEDERATION W E, Association A P H. Standard Methods for the Examination of Water and Wastewater[M]. American Public Health Association (APHA):Washington, DC, 2005.

ZOU J T, PAN J A, WU S Y, et al. Rapid control of activated sludge bulking and simultaneous acceleration of aerobic granulation by adding intact aerobic granular sludge[J]. Science of the Total Environment, 2019, 674:105-113.

TANG P, YU D S, CHEN G H, et al. Novel aerobic granular sludge culture strategy:using granular sludge Anammox process effluent as a biocatalyst[J]. Bioresource Technology, 2019, 294:122156.

郭耀, 李志华, 杨成建, 等. 活性污泥物理结构对呼吸过程的影响[J]. 环境科学, 2019,40(6):323-330.

YANG Q X, ZHAO H L, DU B B. Bacteria and bacteriophage communities in bulking and non-bulking activated sludge in full-scale municipal wastewater treatment systems[J]. Biochemical Engineering Journal, 2017, 119:101-111.

FUKUSHIMA T, WHANG L M, CHEN P C, et al. Linking TFT-LCD wastewater treatment performance to microbial population abundance of Hyphomicrobium and Thiobacillus spp[J]. Bioresource Technology, 2013, 141:131-137.

GUO J, CHENG J P, LI B B, et al. Performance and microbial community in the biocathode of microbial fuel cells under different dissolved oxygen concentrations[J]. Journal of Electroanalytical Chemistry, 2019, 833:433-440.

WANG J, LI Q, QI R, et al. Sludge bulking impact on relevant bacterial populations in a full-scale municipal wastewater treatment plant[J]. Process Biochemistry, 2014, 49(12):2258-2265.

WANG D P, LI T, HUANG K L, et al. Roles and correlations of functional bacteria and genes in the start-up of simultaneous anammox and denitrification system for enhanced nitrogen removal[J]. Science of the Total Environment, 2019, 655:1355-1363.

MARQUES R, RIBERA-GUARDIA A, SANTOS J, et al. Denitrifying capabilities of Tetrasphaera and their contribution towards nitrous oxide production in enhanced biological phosphorus removal processes[J]. Water Research, 2018, 137:262-272.

ZHANG X X, LI A, SZEWZYK U, et al. Improvement of biological nitrogen removal with nitrate-dependent Fe (Ⅱ) oxidation bacterium Aquabacterium parvum B6 in an up-flow bioreactor for wastewater treatment[J]. Bioresource Technology, 2016, 219:624-631.

CHEN C M, MING J, YOZA B A, et al. Characterization of aerobic granular sludge used for the treatment of petroleum wastewater[J]. Bioresource Technology, 2019, 271:353-359.

GU Y Q, LI T T, LI H Q. Biofilm formation monitored by confocal laser scanning microscopy during startup of MBBR operated under different intermittent aeration modes[J]. Process Biochemistry, 2018, 74:132-140.

MULLA S I, HU A Y, WANG Y W, et al. Degradation of triclocarban by a triclosan-degrading Sphingomonas sp. strain YL-JM2C[J]. Chemosphere, 2016, 144:292-296.

SONG G Q, YU Y, LIU T, et al. Performance of microaeration hydrolytic acidification process in the pretreatment of 2-butenal manufacture wastewater[J]. Journal of Hazardous Materials, 2019, 369:465-473.

MA S J, MA H J, HU H D, et al. Effect of mixing intensity on hydrolysis and acidification of sewage sludge in two-stage anaerobic digestion:characteristics of dissolved organic matter and the key microorganisms[J]. Water Research, 2019, 148:359-367.

ZHANG L, LEHMANN K, TOTSCHE K U, et al. Selective successional transport of bacterial populations from rooted agricultural topsoil to deeper layers upon extreme precipitation events[J]. Soil Biology and Biochemistry, 2018, 124:168-178.

Relative Articles

Supplements(0)

Cited By

Proportional views