SHUTTLE CHARACTERISTICS OF LNA AND HNA BACTERIA DURING DENITRIFICATION PROCESS

ZHANG Shuang-shuang; LI Zhi-hua; BEI Yuan; YANG Cheng-jian

doi:10.13205/j.hjgc.202011013

Volume 38 Issue 11

Apr. 2021

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ZHANG Shuang-shuang, LI Zhi-hua, BEI Yuan, YANG Cheng-jian. SHUTTLE CHARACTERISTICS OF LNA AND HNA BACTERIA DURING DENITRIFICATION PROCESS[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(11): 78-84. doi: 10.13205/j.hjgc.202011013

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SHUTTLE CHARACTERISTICS OF LNA AND HNA BACTERIA DURING DENITRIFICATION PROCESS

doi: 10.13205/j.hjgc.202011013

1. Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China;
2. Shanxi Key Laboratory of Environmental Engineering, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China

Received Date: 2020-02-13
Available Online: 2021-04-23
Publish Date: 2021-04-23

Abstract

Abstract

Low nucleic acid (LNA) and high nucleic acid (HNA) bacteria show different characteristics under different environmental conditions, and the effect of denitrification process on bacteria is still unclear. The variations of free-swimming LNA and HNA bacteria under different denitrification conditions and microbial community were thus investigated in this study, it was found that free-swimming bacteria increased rapidly during the denitrification process, and greater the denitrification rate resulted in a higher increase of free-swimming bacteria. It was evidenced that LNA bacteria moved faster than HNA bacteria at the beginning of the denitrification reaction. HNA bacteria showed a rapid increase, either the denitrification reached a certain level, or the floc structure loose or break, indicating that LNA bacteria was mainly on the surface of flocs and functioned as glue, or filling materials of the pores of flocs. HNA bacteria functioned as a backbone of flocs. When starch was used as the carbon source for denitrification, free-swimming bacteria were reduced due to the bridge-capture of starch. However the denitrification of sodium acetate had a more significant effect on free-swimming bacteria than the bridge-capture of starch. In addition, HNA bacteria had higher abundance and diversity than LNA bacteria, and was found out as the main functional bacteria. LNA bacteria can respond faster to denitrification and be used as a signal for denitrification initiation.
- low nucleic acid bacteria (LNA),
- high nucleic acid bacteria (HNA),
- free-swimming bacteria,
- activated sludge,
- denitrification,
- floc structure

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References

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