ISOLATION OF A HETEROTROPHIC NITRIFICATION-AEROBIC DENITRIFYING STRAIN GNR AND ITS APPLICATION OF AMMONIA REMOVAL OF SWINE WASTEWATER

TAN Jun-jie; LI Li-jun; LI Xue-jie; HAN Tian-fang; HU Xiao-min; ZHAO Xin

doi:10.13205/j.hjgc.202102004

Volume 39 Issue 2

Jul. 2021

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TAN Jun-jie, LI Li-jun, LI Xue-jie, HAN Tian-fang, HU Xiao-min, ZHAO Xin. ISOLATION OF A HETEROTROPHIC NITRIFICATION-AEROBIC DENITRIFYING STRAIN GNR AND ITS APPLICATION OF AMMONIA REMOVAL OF SWINE WASTEWATER[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(2): 21-26. doi: 10.13205/j.hjgc.202102004

Citation:

TAN Jun-jie, LI Li-jun, LI Xue-jie, HAN Tian-fang, HU Xiao-min, ZHAO Xin. ISOLATION OF A HETEROTROPHIC NITRIFICATION-AEROBIC DENITRIFYING STRAIN GNR AND ITS APPLICATION OF AMMONIA REMOVAL OF SWINE WASTEWATER[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(2): 21-26. doi: 10.13205/j.hjgc.202102004

Citation:

TAN Jun-jie, LI Li-jun, LI Xue-jie, HAN Tian-fang, HU Xiao-min, ZHAO Xin. ISOLATION OF A HETEROTROPHIC NITRIFICATION-AEROBIC DENITRIFYING STRAIN GNR AND ITS APPLICATION OF AMMONIA REMOVAL OF SWINE WASTEWATER[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(2): 21-26. doi: 10.13205/j.hjgc.202102004

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ISOLATION OF A HETEROTROPHIC NITRIFICATION-AEROBIC DENITRIFYING STRAIN GNR AND ITS APPLICATION OF AMMONIA REMOVAL OF SWINE WASTEWATER

doi: 10.13205/j.hjgc.202102004

1. Department of Environmental Engineering, School of Resources and Civil Engineering, Northeastern University, Shenyang 110819, China;
2. School of Environment and Society, Tokyo Institute of Technology, Tokyo 152-8550, Japan;
3. Bureau of Ecology and Environment of Panjin, Panjin 124010, China

Received Date: 2019-10-28
Available Online: 2021-07-19

Abstract

Abstract

In this study, a heterotrophic nitrification-aerobic denitrifying strain named GNR was isolated from swine wastewater. It was identified as a new strain of genus Acinetobacter through morphological and phylogenetic analysis. The optimal heterotrophic nitrification and aerobic denitrification conditions were investigated as the carbon source of trisodium citrate, C/N ratio of 10:1, initial pH of 8.0 and 6.0, respectively. The maximum removal efficiencies of ammonia and nitrate nitrogen with initial concentration of 50.0 mg/L both reached over 99.0% by strain GNR, after 30℃, 125 r/min shaking cultivation for 72 h. Under the optimal condition, the COD and ammonia removal efficiencies in diluted swine wastewater treatment reached 129, 73 mg/(L·g), respectively. The experimental results indicated that strain GNR had good application potential in wastewater denitrification.
- biological nitrogen removal,
- heterotrophic nitrification,
- aerobic denitrification,
- Acinetobacter,
- swine wastewater

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References(20)

References

[1]	黄廷林, 张丽娜, 张海涵, 等. 一株贫营养异养硝化-好氧反硝化菌的筛选及脱氮特性[J].生态环境学报, 2015,24(1):113-120.
[2]	乔楠, 陈瑞佳, 于大禹. 硅藻土负载异养硝化-好氧反硝化菌的脱氮性能[J]. 化工进展, 2015,34(5):1459-1465.
[3]	王弘宇, 马放, 周丹丹. 同步硝化好氧反硝化生物脱氮机理分析及其研究进展[J]. 四川环境, 2004, 23(6):62-65.
[4]	周少奇, 周吉林. 生物脱氮新技术研究进展[J]. 环境污染治理技术与设备, 2000,1(6):11-19.
[5]	芦婷, 杨璐华, 杨飞飞, 等. 高效反硝化菌强化固相碳源生物脱氮特性研究[J]. 北京大学学报(自然科学版), 2017,53(5):957-963.
[6]	葛启隆, 王国英. 一株好氧反硝化菌的降酚特性及反硝化关键酶活性[J]. 科学技术与工程, 2017,17(24):309-313.
[7]	LESLEY R, JOHANNES G K. Aerobic denitrification:a controversy revived[J]. Archives of Microbiology, 1984, 139(4):351-354.
[8]	YAO S, NI J R, MA T, et al. Heterotrophic nitrification and aerobic denitrification at low temperature by a newly isolated bacterium, Acinetobacter sp.HA2[J]. Bioresource Technology, 2013, 139C(13):80-86.
[9]	ZHANG J B, WU P X, HAO B, et al. Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001[J]. Bioresource Technology, 2011, 102(21):9866-9869.
[10]	苏婉昀, 高俊发, 赵红梅. 异养硝化-好氧反硝化菌的研究进展[J]. 工业水处理, 2013, 33(12):1-5.
[11]	CHEN Q, NI J R. Ammonium removal by Agrobacterium sp. LAD9 capable of heterotrophic nitrification-aerobic denitrification[J]. Journal of Bioscience & Bioengineering, 2012, 113(5):619-623.
[12]	JOO H S, HIRAI M, SHODA M. Piggery wastewater treatment using Alcaligenes faecalis strain No.4 with heterotrophic nitrification and aerobic denitrification[J]. Water Research, 2006, 40(16):3029-3036.
[13]	黄郑郑, 曹刚, 陈海升, 等. 异养硝化-好氧反硝化菌协同竞争对脱氮特性的影响[J]. 环境科学研究, 2017, 30(5):765-774.
[14]	刘芳,赵鑫,潘玉瑾,等. 高效异养硝化细菌富集与强化脱氮[J]. 东南大学学报(自然科学版), 2016, 46(4):807-811.
[15]	FU L L, JIANG B H, LIU J L, et al. Genome sequence analysis of a flocculant-producing bacterium, Paenibacillus shenyangensis[J]. Biotechnology Letters, 2016, 38(3):447-453.
[16]	国家环境保护总局, 水和废水监测分析方法编委会. 水和废水监测分析方法[M]. 4版.北京:中国环境科学出版社, 2002.
[17]	HUANG X F, LI W G, ZHANG D Y, et al. Ammonium removal by a novel oligotrophic Acinetobacter sp. Y16 capable of heterotrophic nitrification-aerobic denitrification at low temperature[J]. Bioresource Technology, 2013, 146(10):44-50.
[18]	王秀杰,王维奇,李军,等. 异养硝化菌Acinetobacter sp.的分离鉴定及其脱氮特性[J]. 中国环境科学, 2017,37(11):4241-4250.
[19]	王田野, 魏荷芬, 胡子全, 等. 一株异养硝化好氧反硝化菌的筛选鉴定及其脱氮特性[J]. 环境科学学报, 2017, 37(3):945-953.
[20]	陈赵芳, 尹立红, 浦跃朴,等. 一株异养硝化菌的筛选及其脱氮条件[J]. 东南大学学报, 2007, 37(3):486-490.