BIOGAS PRODUCTION AND MICROBIAL COMMUNITY SUCCESSION DURING SEQUENCING BATCH ACCLIMATIZATION OF DIGESTED SLUDGE

LIU Xin-yuan; HU Wen-jia; OUYANG Fan; NIE Jia-min; WU Nan; YANG Fan; KONG Si-fang

doi:10.13205/j.hjgc.202103019

Volume 39 Issue 3

Jul. 2021

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2021 > 39(3): 136-141,188

LIU Xin-yuan, HU Wen-jia, OUYANG Fan, NIE Jia-min, WU Nan, YANG Fan, KONG Si-fang. BIOGAS PRODUCTION AND MICROBIAL COMMUNITY SUCCESSION DURING SEQUENCING BATCH ACCLIMATIZATION OF DIGESTED SLUDGE[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(3): 136-141,188. doi: 10.13205/j.hjgc.202103019

Citation:

LIU Xin-yuan, HU Wen-jia, OUYANG Fan, NIE Jia-min, WU Nan, YANG Fan, KONG Si-fang. BIOGAS PRODUCTION AND MICROBIAL COMMUNITY SUCCESSION DURING SEQUENCING BATCH ACCLIMATIZATION OF DIGESTED SLUDGE[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(3): 136-141,188. doi: 10.13205/j.hjgc.202103019

Citation:

LIU Xin-yuan, HU Wen-jia, OUYANG Fan, NIE Jia-min, WU Nan, YANG Fan, KONG Si-fang. BIOGAS PRODUCTION AND MICROBIAL COMMUNITY SUCCESSION DURING SEQUENCING BATCH ACCLIMATIZATION OF DIGESTED SLUDGE[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(3): 136-141,188. doi: 10.13205/j.hjgc.202103019

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BIOGAS PRODUCTION AND MICROBIAL COMMUNITY SUCCESSION DURING SEQUENCING BATCH ACCLIMATIZATION OF DIGESTED SLUDGE

doi: 10.13205/j.hjgc.202103019

1. College of Engineering and Technology, Tianjin Agricultural University, Tianjin 300384, China;
2. Traffic and Environment Department, Shenzhen Institute & Information Technology, Shenzhen 518172, China

Received Date: 2020-02-27
Available Online: 2021-07-19

Abstract

Abstract

This article investigated the adaptability changes of slight-activated seed sludge to new substrate during anaerobic sequencing batch acclimatization. Results showed that the seed sludge had stable methane production ability in the second and third batch after the acclimatization in the first batch, indicating that the effective metabolic adjustment had been obtained in the first batch. According to the results of the pH value and kinetic parameters for methane production in each batch, it was found the delay period for methane production was shortened, the methane production rate was increased and the acidification degree in the initial period of each batch was reduced with the acclimated batch. The results indicated that the adaptability of seed sludge to substrate was gradually enhanced. The microbial community structure before and after sequencing batch culture were detected by high throughput sequencing technology. Results showed that the richness and diversity of acclimated sludge reduced after the three times batch culture. The dominant bacterial genus of Paludibacter with relative abundance of 52.3% and the dominant archaeal genus of Methanosaeta with relative abundance of 72.31%, were selected during the acclimatization.
- digested sludge,
- methane production,
- acclimatization,
- anaerobic digestion,
- community structure

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References(24)

References

[1]	MATHERI A N, NDIWENI S N, BELAID M, et al. Optimising biogas production from anaerobic co-digestion of chicken manure and organic fraction of municipal solid waste[J]. Renewable and Sustainable Energy Reviews, 2017, 80:756-764.
[2]	刘新媛,肖娟,聂家民,等. 鸡粪和餐厨垃圾中温厌氧发酵产甲烷特征及动力学[J]. 中国沼气,2019,37(5):15-20.
[3]	袁悦. 污泥与餐厨垃圾共消化系统启动策略[J]. 环境工程,2018,36(11):137-140 ,75.
[4]	冯磊,徐杰,李润东. 餐厨垃圾中温厌氧发酵接种污泥的驯化研究[J]. 可再生能源,2013,31(10):98-102 ,108.
[5]	裴梦富,强虹,杨祎楠,等. 利用逐级提高进料浓度的方法启动完全混合反应器处理鸡粪[J]. 环境工程学报,2018,12(6):1825-1832.
[6]	WANG M, SUN X L, LI P F, et al. A novel alternate feeding mode for semi-continuous anaerobic co-digestion of food waste with chicken manure[J]. Bioresource Technology, 2014, 164:309-314.
[7]	KURADE M B, SAHA S, KIM J R, et al. Microbial community acclimatization for enhancement in the methane productivity of anaerobic co-digestion of fats, oil, and grease[J]. Bioresource Technology, 2020, 296:122294.
[8]	ZHANG H, WU J W, GAO L J, et al. Aerobic deterioration of corn stalk silage and its effect on methane production and microbial community dynamics in anaerobic digestion[J]. Bioresource Technology, 2018, 250:828-837.
[9]	王旭辉,徐鑫,宝哲,等. 高通量测序分析玉米秸秆与牛粪联合发酵阶段微生物多样性变化[J]. 食品与发酵工业,2019,45(3):47-55.
[10]	吴坤君, 龚佩瑜, 盛承发. 昆虫多样性参数的测定和表达[J]. 昆虫, 2005(3):338-340.
[11]	孟颖,唐明跃,孙芳青,等. 餐厨、果蔬与鸡粪多元混合物料厌氧消化实验研究[J]. 中国沼气,2013,31(4):12-16 ,48.
[12]	ROSALINDA C AND SIMÓN G M. Start-up of dry semi-continuous OFMSW fermentation for methane production[J]. Biomass and Bioenergy, 2020, 136:105544.
[13]	DING H B, LIU X Y, STABNIKOVA O, et al. Effect of protein on biohydrogen production from starch of food waste[J]. Water Science & Technology, 2008, 57(7):1031-1036.
[14]	LIU C, LI H, ZHANG Y Y, et al. Evolution of microbial community along with increasing solid concentration during high-solids anaerobic digestion of sewage sludge[J]. Bioresource Technology, 2016, 216:87-94.
[15]	KADNIKOV V V, MARDANOV A V, BELETSKY A V, et al. Genome of the candidate phylum Aminicenantes bacterium from a deep subsurface thermal aquifer revealed its fermentative saccharolytic lifestyle[J]. Extremophiles, 2019, 23(2):189-200.
[16]	TAO Y, ERSAHIN M E, GHASIMI D S M, et al. Biogas productivity of anaerobic digestion process is governed by a core bacterial microbiota[J]. Chemical Engineering Journal, 2020, 380:122425.
[17]	PAN J T, MA J Y, ZHAI L M, et al. Enhanced methane production and syntrophic connection between microorganisms during semi-continuous anaerobic digestion of chicken manure by adding biochar[J]. Journal of Cleaner Production, 2019, 240:118178.
[18]	QIU Y L, KUANG X Z, SHI X S, et al. Paludibacter jiangxiensis sp. nov., a strictly anaerobic, propionate-producing bacterium isolated from rice paddy field[J]. Archives of Microbiology, 2014, 196(3):149-155.
[19]	徐恒,汪翠萍,颜锟,等. 颗粒型厌氧生物膜改善高氢分压下丙酸降解抑制研究[J]. 中国环境科学,2016,36(5):1435-1441.
[20]	IMACHI H, SAKAI S M[M]. Bergey's Manual of Systematics of Archaea and Bacteria, 2015:1-4.
[21]	王洋,李海红,常华.餐厨垃圾与固体废物共消化产气效果研究[J].化学工程,2019,47(3):1-6 ,18.
[22]	KERSTIN M, SHARIF A, MARIAN K. Beneficial effects of intermittent feedstock management on biogas and methane production[J]. Bioresource Technology, 2020, 304:123004.
[23]	XING B S, HAN Y, WANG X C, et al. Cow manure as additive to a DMBR for stable and high-rate digestion of food waste:performance and microbial community[J]. Water Research, 2020, 168:115099.
[24]	朱铁群,李凯慧,张杰. 活性污泥驯化的微生物生态学原理[J]. 微生物学通报,2008,35(6):939-943.