MICROORGANISM COMMUNITY STRUCTURE AND MICROBIOLOGICAL DETERIORATION IN HETEROGENEOUS SITES CONTAMINATED WITH PETROLEUM HYDROCARBON

XUE Zhen-kun; ZUO Rui; WANG Jin-sheng; CHEN Min-hua; MENG Li; JIN Chao

doi:10.13205/j.hjgc.202108026

Volume 39 Issue 8

Jan. 2022

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XUE Zhen-kun, ZUO Rui, WANG Jin-sheng, CHEN Min-hua, MENG Li, JIN Chao. MICROORGANISM COMMUNITY STRUCTURE AND MICROBIOLOGICAL DETERIORATION IN HETEROGENEOUS SITES CONTAMINATED WITH PETROLEUM HYDROCARBON[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(8): 188-196. doi: 10.13205/j.hjgc.202108026

Citation:

XUE Zhen-kun, ZUO Rui, WANG Jin-sheng, CHEN Min-hua, MENG Li, JIN Chao. MICROORGANISM COMMUNITY STRUCTURE AND MICROBIOLOGICAL DETERIORATION IN HETEROGENEOUS SITES CONTAMINATED WITH PETROLEUM HYDROCARBON[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(8): 188-196. doi: 10.13205/j.hjgc.202108026

Citation:

XUE Zhen-kun, ZUO Rui, WANG Jin-sheng, CHEN Min-hua, MENG Li, JIN Chao. MICROORGANISM COMMUNITY STRUCTURE AND MICROBIOLOGICAL DETERIORATION IN HETEROGENEOUS SITES CONTAMINATED WITH PETROLEUM HYDROCARBON[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(8): 188-196. doi: 10.13205/j.hjgc.202108026

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MICROORGANISM COMMUNITY STRUCTURE AND MICROBIOLOGICAL DETERIORATION IN HETEROGENEOUS SITES CONTAMINATED WITH PETROLEUM HYDROCARBON

doi: 10.13205/j.hjgc.202108026

XUE Zhen-kun^1,2,
ZUO Rui^{1,2
,
,},
WANG Jin-sheng^1,2,
CHEN Min-hua^1,2,
MENG Li^1,2,
JIN Chao^1,2

1. College of Water Science, Beijing Normal University, Beijing 100875, China;
2. Engineering Research Center of Ground Ministry of Education, Beijing 100875, China

Received Date: 2019-09-27
Available Online: 2022-01-18

Abstract

Abstract

In order to explore the degree of biodegradation and analyze the possibility of microbial remediation in the heterogeneous site contaminated by petroleum hydrocarbon, the upstream zone of the Chaobai River was selected as a typical research area. Under the control of an alluvial fan, the research area was characterized by a strong heterogeneity and a deep underground water depth. High-throughput sequencing analysis and microbial degradation column experiment were carried out by collecting samples from the contaminated sites. High-throughput sequencing results showed that Firimicutes (40.1%) was the dominant phylum and Paenisporosarcina (34.8%) was the dominant genus in uncontaminated area (T1). The dominant phylum identified in other samples of the site was Proteobacteria (35.1%~52.2%) and the dominant genus was Bacillius (7.7%~16.8%). Petroleum hydrocarbon pollutants greatly changed the microbial community structure and diversity of the region, and burial depth and water bearing conditions were significant influencing factors. The results of the column experiment of indoor microbial petroleum hydrocarbon degradation showed that adsorption was the main fate of petroleum hydrocarbon pollutants in the initial stage. The microbial degradation became stronger in the stage of 0~200 h, which played a leading role of this stage but the removal rate was lower than the adsorption. The results showed that the proportion and number of the dominant bacteria of petroleum hydrocarbon degradation were both low in the heterogeneous sites contaminated by petroleum hydrocarbon, and the microbial degradation was weak under natural condition.
- soil petroleum hydrocarbon,
- community structure,
- biodiversity,
- micro-biological degradation

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References(35)

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