INACTIVATION AND REACTIVATION OF ANTIBIOTIC-RESISTANT BACTERIA IN FILTERED SECONDARY EFFLUENT OF WASTEWATER TREATMENT PLANT DURING AND AFTER UV DISINFECTION

LI Teng; HU Jing; SONG Haiwang; QIN Xianxiang; CHENG Lihua; BI Xuejun

doi:10.13205/j.hjgc.202202003

Volume 40 Issue 2

Apr. 2022

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LI Teng, HU Jing, SONG Haiwang, QIN Xianxiang, CHENG Lihua, BI Xuejun. INACTIVATION AND REACTIVATION OF ANTIBIOTIC-RESISTANT BACTERIA IN FILTERED SECONDARY EFFLUENT OF WASTEWATER TREATMENT PLANT DURING AND AFTER UV DISINFECTION[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(2): 14-19,41. doi: 10.13205/j.hjgc.202202003

Citation:

LI Teng, HU Jing, SONG Haiwang, QIN Xianxiang, CHENG Lihua, BI Xuejun. INACTIVATION AND REACTIVATION OF ANTIBIOTIC-RESISTANT BACTERIA IN FILTERED SECONDARY EFFLUENT OF WASTEWATER TREATMENT PLANT DURING AND AFTER UV DISINFECTION[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(2): 14-19,41. doi: 10.13205/j.hjgc.202202003

Citation:

LI Teng, HU Jing, SONG Haiwang, QIN Xianxiang, CHENG Lihua, BI Xuejun. INACTIVATION AND REACTIVATION OF ANTIBIOTIC-RESISTANT BACTERIA IN FILTERED SECONDARY EFFLUENT OF WASTEWATER TREATMENT PLANT DURING AND AFTER UV DISINFECTION[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(2): 14-19,41. doi: 10.13205/j.hjgc.202202003

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INACTIVATION AND REACTIVATION OF ANTIBIOTIC-RESISTANT BACTERIA IN FILTERED SECONDARY EFFLUENT OF WASTEWATER TREATMENT PLANT DURING AND AFTER UV DISINFECTION

doi: 10.13205/j.hjgc.202202003

1. School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266033, China;
2. State and Local Joint Engineering Center of Urban Sewage Treatment and Recycling, Qingdao 266033, China

Received Date: 2021-02-20
Available Online: 2022-04-02
Publish Date: 2022-04-02

Abstract

Abstract

Antibiotic resistant bacteria(ARB) have been widely concerned as an emerging contaminant, but the effect of ultraviolet(UV) disinfection on ARB removal has not been fully studied. This paper focused on the disinfection of the filtered secondary effluent from a municipal sewage treatment plant, investigated the removal effect of UV disinfection on ARB and the photoreactivation and dark repair potential of ARB by analyzing the abundance of bacteria with resistance to tetracycline, ampicillin, chloramphenicol or streptomycin before and after UV disinfection. The results showed that UV could partly inactivate the ARB, and the resistance ability of the four ARB to UV disinfection followed the order of ampicillin resistant bacteria>tetracycline resistant bacteria>streptomycin resistant bacteria≈chloramphenico resistant bacteria. UV disinfection with dosage of 20 mJ/cm² could completely inactivate chloramphenicol resistant bacteria and streptomycin resistant bacteria, and these two kinds of ARB did not appear photoreactivation or dark repair ability within 24 h post disinfection. This dosage of UV could not completely inactivate ampicillin resistant bacteria or tetracycline resistant bacteria, and the inactivated bacteria could partly reactivate with or without the light. When UV dosage reached 80 mJ/cm², these four kinds of ARB were totally inactivated. Ampicillin resistant bacteria showed the reactivation ability within 6 h post disinfection. Whether putting in light or in dark for 24 h after disinfection, more than 70.32% of the total heterotrophic bacteria in water sample disinfected by UV were resistant to ampicillin. Therefore, UV disinfection alone could not effectively control the spread of ARB from sewage treatment plants to the environment.
- UV disinfection,
- total heterotrophic bacteria,
- antibiotic resistant bacteria,
- photoreactivation,
- dark repair

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References(21)

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