REMOVAL OF ANTIBIOTIC RESISTANCE GENES DURING THE TREATMENT OF SWINE WASTEWATER BY MBR

DUAN Tong; ZENG Xiaoyun; TAN Shucheng

doi:10.13205/j.hjgc.202204002

Volume 40 Issue 4

Apr. 2022

Turn off MathJax

Article Contents

Article Navigation > ENVIRONMENTAL ENGINEERING > 2022 > 40(4): 8-13,21

DUAN Tong, ZENG Xiaoyun, TAN Shucheng. REMOVAL OF ANTIBIOTIC RESISTANCE GENES DURING THE TREATMENT OF SWINE WASTEWATER BY MBR[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(4): 8-13,21. doi: 10.13205/j.hjgc.202204002

Citation:

DUAN Tong, ZENG Xiaoyun, TAN Shucheng. REMOVAL OF ANTIBIOTIC RESISTANCE GENES DURING THE TREATMENT OF SWINE WASTEWATER BY MBR[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(4): 8-13,21. doi: 10.13205/j.hjgc.202204002

Citation:

PDF( 5916 KB)

REMOVAL OF ANTIBIOTIC RESISTANCE GENES DURING THE TREATMENT OF SWINE WASTEWATER BY MBR

doi: 10.13205/j.hjgc.202204002

1. Institute of International Rivers and Eco-Security, Yunnan University, Kunming 650500, China;
2. Wuhu Qingyi Environmental Protection Co., Ltd, Shanghai 200050, China

Received Date: 2021-03-23
Available Online: 2022-07-06

Abstract

Abstract

In this study antibiotic resistant genes (ARGs) in swine wastewater during the biological treatment was investigated through the methods of laboratory experiment. The ARGs removal during the biological treatment as well as the parameter optimization were explored, in order to provide the theoretical and technological support for control and elimination of ARGs in swine waste. A lab-scale membrane bio-reactor (MBR) was established to treat swine wastewater. The mean removal rate of COD and TN were 95.5% and 92.4%. At the longer SRT of 30, MBR showed the highest removal efficiency of ARGs copy number between 1.5 and 2 logs,but the removal efficiency can reach up to 3.78 logs when the SRT is shortened to 15 days. The effects of environmental factors(DO、pH、T、SS、COD、NH⁺₄-N and TN) on the copy number and abundance of ARGs in MBR effluent showed the same trend. Therefore, by reducing SRT, increasing DO, pH, and temperature, the depletion efficiency of ARGs copy number and abundance can be improved, which has practical application value and guiding significance.
- membrane bio-reactor,
- swine wastewater,
- antibiotic resistant genes,
- environmental factors

FullText(HTML)

References(32)

References

[1]	BARTON M D. Antibiotic use in animal feed and its impact on human healt[J]. Nutrition Research Reviews, 2000, 13(2):279-299.
[2]	KIM S, JENSEN J N, AGA D S, et al. Tetracycline as a selector for resistant bacteria in activated sludge[J]. Chemosphere, 2007, 66(9):1643-1651.
[3]	闫雷,闫璐,孙睿,等.碱预处理对污泥中抗生素抗性基因丰度的影响[J].中国给水排水, 2014, 30(19):99-102.
[4]	BAQUERO F, MARTINEZ J L, CANTON R. Antibiotics and antibiotic resistance in water environments[J]. Current Opinion in Biotechnology, 2008, 19(3):260-265.
[5]	于帅,李锦,毛大庆,等.抗生素抗性基因在废(污)水处理系统的来源、传播扩散、归趋以及污染控制研究进展[J].环境化学, 2013,32(11):2059-2071.
[6]	MICHAEL I, RIZZO L, MCARDELL C S, et al. Urban wastewater treatment plants as hotspots for the release of antibiotics in the environment:a review[J]. Water Research, 2013, 47(3):957-995.
[7]	隋倩雯,张俊亚,魏源送,等.畜禽养殖废水生物处理与农田利用过程抗生素抗性基因的转归特征研究进展[J].环境科学学报, 2016,36(1):16-26.
[8]	BOERJESSON S, MATTSSON A, LINDGREN P E. Genes encoding tetracycline resistance in a full-scale municipal wastewater treatment plant investigated during one year[J]. Journal of Water and Health, 2010, 8(2):247-256.
[9]	MUNIR M, WONG K, XAGORARAKI I. Release of antibiotic resistant bacteria and genes in the effluent and biosolids of five wastewater utilities in Michigan[J]. Water Research, 2011, 45(2):681-693.
[10]	闫立娜,王朝朝,李思敏,等.中空纤维MBR工艺膜堵塞行为与膜渗透性评估[J].中国给水排水, 2017, 33(7):15-22.
[11]	沈瀚,王亮,李明德,等.填料型A/O工艺处理养猪废水中试研究[J].中国给水排水, 2016, 32(7):121-125.
[12]	庞小可,许继飞,张秋萍,等.添加四环素和重金属锌对牛粪厌氧消化性能及四环素类抗性基因丰度的影响[J].环境科学学报,2021,41(4):1487-1495.
[13]	APLEY M D, BUSH E J, MORRISON R B,et al.Use estimates of in-feed antimicrobials in swine production in the United States[J]. Foodborne Pathogens and Disease, 2012, 9(3):272-279.
[14]	NG L K, MARTIN I, ALFA M,et al.Multiplex PCR for the detection of tetracycline resistant genes[J]. Molecular and Cellular Probes, 2001, 15(4):209-215.
[15]	CHEN J, YU Z, MICHEL F C, et al. Development and application of real-time PCR assays for quantification of erm genes conferring resistance to macrolides-lincosamides-streptogramin B in livestock manure and manure management systems[J]. Applied and Environmental Microbiology, 2007, 73(14):4407-4416.
[16]	SUTCLIFFE J, GREBE T, TAIT-KAMRADT A, et al. Detection of erythromycin-resistant determinants by PCR[J]. Antimicrobial agents and chemotherapy, 1996, 40(11):2562-2566.
[17]	VOLOKHOV D, CHIZHIKOV V, CHUMAKOV K,et al.Microarray analysis of erythromycin resistance determinants[J]. Journal of Applied Microbiology, 2003, 95(4):787-798.
[18]	COLOM K, PÉREZ J, ALONSO R, et al. Simple and reliable multiplex PCR assay for detection of bla TEM, bla SHV and bla OXA-1 genes in Enterobacteriaceae[J]. FEMS Microbiology Letters, 2003, 223(2):147-151.
[19]	STOKES H W, NESBØC L, HOLLEY M, et al. Class 1 integrons potentially predating the association with Tn402-like transposition genes are present in a sediment microbial community[J]. Journal of Bacteriology, 2006, 188(16):5722-5730.
[20]	RATHER M A, AULAKH R S, GILL J P S, et al. Detection and sequencing of plasmid encoded tetracycline resistance determinants (tetA and tetB) from food-borne Bacillus cereus isolates[J]. Asian Pacific Journal of Tropical Medicine, 2012, 5(9):709-712.
[21]	冀秀玲,刘芳,沈群辉,等.养殖场废水中磺胺类和四环素抗生素及其抗性基因的定量检测[J].生态环境学报, 2011,20(5):927-933.
[22]	CHEN B W, YANG Y, LIANG X M, et al. Metagenomic profiles of antibiotic resistance genes (ARGs) between human impacted estuary and deep ocean sediments[J]. Environmental Science&Technology, 2013, 47(22):12753-12760.
[23]	TAO C W, HSU B M, JI W T, et al. Evaluation of five antibiotic resistance genes in wastewater treatment systems of swine farms by real-time PCR[J]. Science of the Total Environment, 2014, 496:116-121.
[24]	AALI R, NIKAEEN M, KHANAHMAD H, et al. Monitoring and comparison of antibiotic resistant bacteria and their resistance genes in municipal and hospital wastewaters[J]. International Journal of Preventive Medicine, 2014, 5(7):887-94.
[25]	JOY S R, BARTELT-HUNT S L, SNOW D D, et al.Fate and transport of antimicrobials and antimicrobial resistance genes in soil and runoff following land application of swine manure slurry[J]. Environmental Science&Technology, 2013, 47(21):12081-12088.
[26]	CHENG W, CHEN H, SU C, et al.Abundance and persistence of antibiotic resistance genes in livestock farms:a comprehensive investigation in eastern China[J]. Environment International, 2013, 61:1-7.
[27]	MCKINNEY C W, LOFTIN K A, MEYER M T, et al.Tet and sul antibiotic resistance genes in livestock lagoons of various operation type, configuration, and antibiotic occurrence[J]. Environmental Science&Technology, 2010, 44(16):6102-6109.
[28]	BARKOVSKII A, MANOYLOV K, BRIDGES C. Positive and negative selection towards tetracycline resistance genes in manure treatment lagoons[J]. Journal of Applied Microbiology, 2012, 112(5):907-919.
[29]	HUOVINEN P, SUNDSTRÖM L, SWEDBERG G, et al.Trimethoprim and sulfonamide resistance[J]. Antimicrobial Agents and Chemotherapy, 1995, 39(2):279.
[30]	ZANETTI L, FRISON N, NOTA E, et al.Progress in real-time control applied to biological nitrogen removal from wastewater:a short-review[J]. Desalination, 2012, 286:1-7.
[31]	MA L, ZHANG X X, ZHAO F, et al. Sewage treatment plant serves as a hot-spot reservoir of integrons and gene cassettes[J]. Journal of Environmental Biology, 2013, 34(2):391-399.
[32]	SUI Q, CHAO J, ZHANG J, et al. Does the biological treatment or membrane separation reduce the antibiotic resistance genes from swine wastewater through a sequencing-batch membrane bioreactor treatment process[J]. Environment International, 2018, 118(SEP.):274-281.