EFFECTS OF TYPICAL ANTIBIOTICS ON METHANOGENESIS EFFICIENCY AND MICROBIAL METABOLITES DURING ANAEROBIC DIGESTION OF PIG MANURE

CHEN Yuqi; ZHANG Yanlong; WANG Yuzheng; LI Junxin; CAO Wenzhi

doi:10.13205/j.hjgc.202204013

Volume 40 Issue 4

Apr. 2022

Turn off MathJax

Article Contents

Article Navigation > ENVIRONMENTAL ENGINEERING > 2022 > 40(4): 89-96,120

CHEN Yuqi, ZHANG Yanlong, WANG Yuzheng, LI Junxin, CAO Wenzhi. EFFECTS OF TYPICAL ANTIBIOTICS ON METHANOGENESIS EFFICIENCY AND MICROBIAL METABOLITES DURING ANAEROBIC DIGESTION OF PIG MANURE[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(4): 89-96,120. doi: 10.13205/j.hjgc.202204013

Citation:

CHEN Yuqi, ZHANG Yanlong, WANG Yuzheng, LI Junxin, CAO Wenzhi. EFFECTS OF TYPICAL ANTIBIOTICS ON METHANOGENESIS EFFICIENCY AND MICROBIAL METABOLITES DURING ANAEROBIC DIGESTION OF PIG MANURE[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(4): 89-96,120. doi: 10.13205/j.hjgc.202204013

Citation:

CHEN Yuqi, ZHANG Yanlong, WANG Yuzheng, LI Junxin, CAO Wenzhi. EFFECTS OF TYPICAL ANTIBIOTICS ON METHANOGENESIS EFFICIENCY AND MICROBIAL METABOLITES DURING ANAEROBIC DIGESTION OF PIG MANURE[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(4): 89-96,120. doi: 10.13205/j.hjgc.202204013

PDF( 3769 KB)

EFFECTS OF TYPICAL ANTIBIOTICS ON METHANOGENESIS EFFICIENCY AND MICROBIAL METABOLITES DURING ANAEROBIC DIGESTION OF PIG MANURE

doi: 10.13205/j.hjgc.202204013

1. College of Environment and Ecology of Xiamen University, Xiamen 361101, China;
2. Investment & Development Co., Ltd. of China Construction Fourth Bureau, Xiamen 361000 China

Received Date: 2021-04-28
Available Online: 2022-07-06

Abstract

Abstract

In order to clarify the effects of antibiotics on anaerobic digestion of pig manure, this study focused on methane production potential, metabolism, and byproducts of anaerobic digestion under the treatment of three typical antibiotics. The methane production potential of tetracycline and sulfadiazine treatment groups was about 60.92~67.00 mL/ g TS and 55.88~62.13 mL/g TS respectively, higher than that in control group, 58.15 mL/g TS. The methane production potential of oxytetracycline treatment group was 42.27~50.43 mL/g TS, while 16%~19% of COD in the system was converted into CH₄. The addition of oxytetracycline promoted the hydrolysis stage, while the acidogenesis stage and methanogenesis stage were inhibited. Addition of the three antibiotics promoted the transformation of dissolved proteins while inhibited that of the dissolved polysaccharides. Further analysis of dissolved organic matter showed that humic acid content after oxytetracycline treatment was lower than others. The addition of different antibiotics affected the metabolites and pathways of anaerobic digestion, resulting in positive or negative effect on CH₄ production and degradation of organic components.
- anaerobic digestion,
- pig manure,
- veterinary antibiotics,
- methanogenesis efficiency,
- metabolites

FullText(HTML)

References(45)

References

[1]	辛良杰,王佳月,王立新.基于居民膳食结构演变的中国粮食需求量研究[J].资源科学, 2015, 37(7):1347-1356.
[2]	DONG W L, WANG X B, YANG J. Future perspective of China's feed demand and supply during its fast transition period of food consumption[J]. Journal of Integrative Agriculture, 2015, 14(6):1092-1100.
[3]	王明利.改革开放四十年我国畜牧业发展:成就、经验及未来趋势[J].农业经济问题, 2018(8):60-70.
[4]	何亮凤.兽药使用不当给畜禽及其产品带来的危害[J].中国畜禽种业, 2020, 16(1):65.
[5]	陈佳静,张冬冬,赖兴富,等.常用抗生素在鸡体内消除和鸡蛋中残留的规律研究进展[J].中国家禽, 2020, 42(3):82-91.
[6]	闫琦,刘培培,张娇娇,等.畜禽粪便中残留四环素类抗生素的研究概况[J].家畜生态学报, 2018, 39(5):80-86.
[7]	CHEN L L, CONG R G, SHU B R, et al. A sustainable biogas model in China:the case study of Beijing Deqingyuan biogas project[J]. Renewable and Sustainable Energy Reviews, 2017, 78:773-779.
[8]	汪少娜,周北海.磺胺类抗生素对猪粪厌氧发酵的影响及其降解机理研究[D].北京:北京科技大学, 2020.
[9]	卫旻,张无敌,尹芳.兽用四环素类抗生素对猪粪厌氧消化的影响[D].昆明:云南师范大学, 2019.
[10]	XIONG Y H, HARB M, HONG P Y. Performance and microbial community variations of anaerobic digesters under increasing tetracycline concentrations[J]. Applied Microbiology and Biotechnology, 2017, 101(13):5505-5517.
[11]	MUSTAPHA N A, SAKAI K, SHIRAI Y, et al. Impact of different antibiotics on methane production using waste-activated sludge:mechanisms and microbial community dynamics[J]. Applied Microbiology and Biotechnology, 2016, 100(21):9355-9364.
[12]	AYDIN S, INCE B, CETECIOGLU Z, et al. Performance of anaerobic sequencing batch reactor in the treatment of pharmaceutical wastewater containing erythromycin and sulfamethoxazole mixture[J]. Water Science and Technology, 2014, 70(10):1625-1632.
[13]	SHIMADA T, LI X, ZILLES J L, et al. Effects of the antimicrobial tylosin on the microbial community structure of an anaerobic sequencing batch reactor[J]. Biotechnology and Bioengineering, 2011, 108(2):296-305.
[14]	AYDIN S, INCE B, CETECIOGLU Z, et al. Combined effect of erythromycin, tetracycline and sulfamethoxazole on performance of anaerobic sequencing batch reactors[J]. Bioresource Technology, 2015, 186:207-214.
[15]	AYDIN S, CETECIOGLU Z, ARIKAN O, et al. Inhibitory effects of antibiotic combinations on syntrophic bacteria, homoacetogens and methanogens[J]. Chemosphere, 2015, 120:515-520.
[16]	聂宇,陈娅婷,孙照勇,等.污水/城市污泥中抗生素对厌氧消化的影响研究进展[J].应用与环境生物学报, 2020, 26(2):479-488.
[17]	GILCREAS F W. Standard methods for the examination of water and waste water[J]. American Journal of Public Health and the Nations Health, 1966, 56(3):387-388.
[18]	LI Q, QIAO W, WANG X C, et al. Kinetic characterization of thermophilic and mesophilic anaerobic digestion for coffee grounds and waste activated sludge[J]. Waste Management, 2015, 36:77-85.
[19]	ZHAO S N, LI P, FANG H L, et al. Enhancement methane fermentation of Enteromorpha prolifera waste by Saccharomyces cerevisiae:batch kinetic investigation, dissolved organic matter characterization, and synergistic mechanism[J]. Environmental Science and Pollution Research, 2020, 27(14):16254-16267.
[20]	HE Y P, TIAN Z, YI Q Z, et al. Impact of oxytetracycline on anaerobic wastewater treatment and mitigation using enhanced hydrolysis pretreatment[J]. Water Research, 2020, 187:116408.
[21]	ZWIETERING M H, JONGENBURGER I, ROMBOUTS F M, et al. Modeling of the bacterial growth curve[J]. Applied and Environmental Microbiology, 1990, 56(6):1875.
[22]	TANG R, YUAN S J, CHEN F Q, et al. Effects of roxarsone and sulfadiazine on biogas production and their degradation during anaerobic digestion[J]. International Biodeterioration&Biodegradation, 2019, 140:113-118.
[23]	余博,田哲,池勇志,等.土霉素对剩余污泥中温厌氧消化的短期和长期影响[J].环境工程学报, 2016, 10(4):2009-2015.
[24]	ZHI S L, LI Q, YANG F X, et al. How methane yield, crucial parameters and microbial communities respond to the stimulating effect of antibiotics during high solid anaerobic digestion[J]. Bioresource Technology, 2019, 283:286-296.
[25]	CETECIOGLU Z, INCE B, GROS M, et al. Chronic impact of tetracycline on the biodegradation of an organic substrate mixture under anaerobic conditions[J]. Water Research, 2013, 47(9):2959-2969.
[26]	强虹,杨祎楠,李娜,等.金霉素浓度对鸡粪中温厌氧消化特性及抗生素降解的影响[J].农业工程学报, 2019, 35(10):181-190.
[27]	解永磊,秦松岩. UASB-A/O-Fenton组合工艺处理四环素类抗生素废水试验研究[D].天津:天津理工大学, 2015.
[28]	王祥清,马三剑.兽用抗生素对猪粪厌氧消化的抑制效应[D].苏州:苏州科技大学, 2019.
[29]	杨侠,李茹莹;杜连柱.污泥厌氧消化系统特征参数对抗生素降解迁移的影响研究[D].天津:天津大学, 2018.
[30]	张鑫,张社奇;谷洁.抗生素对牛粪厌氧发酵功能微生物及抗性基因影响机理研究[D].杨凌:西北农林科技大学, 2019.
[31]	ZHANG L L, YUE Q Y, YANG K L, et al. Enhanced phosphorus and ciprofloxacin removal in a modified BAF system by configuring Fe-C micro electrolysis:investigation on pollutants removal and degradation mechanisms[J]. Journal of Hazardous Materials, 2018, 342:705-714.
[32]	MOLINUEVO-SALCES B, GÓMEZ X, MORÁN A, et al. Anaerobic co-digestion of livestock and vegetable processing wastes:fibre degradation and digestate stability[J]. Waste Management, 2013, 33(6):1332-1338.
[33]	叶超,陈毅刚.抗生素对活性污泥系统运行效果及代谢产物的影响:以氧氟沙星为例[J].环境科技, 2020, 33(4):29-33.
[34]	楼成珂,孙培德,王如意,等.土霉素对SBR系统细菌的抑制效应与机制研究[J].环境科学学报, 2017, 37(7):2552-2560.
[35]	史金才,廖新俤,吴银宝. 4种畜禽粪便厌氧发酵产甲烷特性研究[J].中国生态农业学报, 2010, 18(3):632-636.
[36]	汪刚慧,刘宏波,郑志永,等.利用米曲霉发酵餐厨垃圾产水解酶促进污泥厌氧消化[J].环境工程学报, 2019, 13(5):1175-1185.
[37]	DU H X, LI F S. Characteristics of dissolved organic matter formed in aerobic and anaerobic digestion of excess activated sludge[J]. Chemosphere, 2017, 168:1022-1031.
[38]	WANG X Q, MUHMOOD A, LYU T, et al. Mechanisms of genuine humic acid evolution and its dynamic interaction with methane production in anaerobic digestion processes[J]. Chemical Engineering Journal, 2021, 408:127322.
[39]	FERNANDES T V, van LIER J B, ZEEMAN G. Humic acid-like and fulvic acid-like inhibition on the hydrolysis of cellulose and tributyrin[J]. BioEnergy Research, 2015, 8(2):821-831.
[40]	LI J, HAO X D, van LOOSDRECHT M C M, et al. Effect of humic acids on batch anaerobic digestion of excess sludge[J]. Water Research, 2019, 155:431-443.
[41]	TANG Y F, LI X W, DONG B, et al. Effect of aromatic repolymerization of humic acid-like fraction on digestate phytotoxicity reduction during high-solid anaerobic digestion for stabilization treatment of sewage sludge[J]. Water Research, 2018, 143:436-444.
[42]	ZHOU W L, WU B T, DONG X J, et al. Effect of nutrient supply on the production of soluble microbial products (SMP) in anaerobic reactors[J]. Fermentation Technology, 2015, 4(2):1-8.
[43]	GAO X T, TAN W B, ZHAO Y, et al. Diversity in the mechanisms of humin formation during composting with different materials[J]. Environmental Science&Technology, 2019, 53(7):3653-3662.
[44]	郝晓地,周鹏,曹亚莉.污水处理中腐殖质的来源及其演变过程[J].环境工程学报, 2017, 11(1):1-11.
[45]	周鹏,郝晓地.污泥厌氧消化过程腐殖质表征及其演变研究[D].北京:北京建筑大学, 2017.