EXPERIMENTAL STUDY ON CO<sub>2</sub> FIXATION COUPLED WITH WASTEWATER PURIFICATION BY <i>CHLORELLA VULGARIS</i> UNDER DIFFERENT AMMONIUM CONCENTRATIONS

KONG Jia; SHEN Bo-xiong; KONG Wen-wen; DAN Kai-xuan

doi:10.13205/j.hjgc.202205002

Volume 40 Issue 5

Jul. 2022

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KONG Jia, SHEN Bo-xiong, KONG Wen-wen, DAN Kai-xuan. EXPERIMENTAL STUDY ON CO2 FIXATION COUPLED WITH WASTEWATER PURIFICATION BY CHLORELLA VULGARIS UNDER DIFFERENT AMMONIUM CONCENTRATIONS[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(5): 9-17,94. doi: 10.13205/j.hjgc.202205002

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KONG Jia, SHEN Bo-xiong, KONG Wen-wen, DAN Kai-xuan. EXPERIMENTAL STUDY ON CO₂ FIXATION COUPLED WITH WASTEWATER PURIFICATION BY CHLORELLA VULGARIS UNDER DIFFERENT AMMONIUM CONCENTRATIONS[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(5): 9-17,94. doi: 10.13205/j.hjgc.202205002

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EXPERIMENTAL STUDY ON CO₂ FIXATION COUPLED WITH WASTEWATER PURIFICATION BY CHLORELLA VULGARIS UNDER DIFFERENT AMMONIUM CONCENTRATIONS

doi: 10.13205/j.hjgc.202205002

KONG Jia^1,2,
SHEN Bo-xiong^{1,2,3
,},
KONG Wen-wen^{1,2
,},
DAN Kai-xuan^1,2

1. School of Energy and Environmental Engineering, Hebei University of Technology, Tianjin 300401, China;
2. Tianjin Key Laboratory of Clean Energy Utilization and Pollutant Control, Tianjin 300401, China;
3. School of Chemical Engineering, Hebei University of Technology, Tianjin 300401, China

Received Date: 2021-07-06
Available Online: 2022-07-02

Abstract

Abstract

To explore the potential of Chlorella vulgaris(C. vulgaris) in fixing CO₂, purifying wastewater and producing protein simultaneously, the effects of different ammonium concentrations(30 mg/L, 60 mg/L, 90 mg/L NH₄Cl) and CO₂ concentrations(0.038% and 10%) on the growth, CO₂ fixation, nutrients removal and protein production of C. vulgaris were studied. The combination of Logistic function and the modified Monod function was also used to describe the relationship between C. vulgaris specific growth rate and concentration of ammonium(NH⁺₄-N) and phosphate(PO^3-₄-P), respectively.Resultsshowed that the biomass of 10% CO₂ group(380.16~499.52 mg/L) was 3.54~8.30 times that of 0.038% CO₂ group(44.73~120.00 mg/L). Meanwhile, the consumption rates of ammonium and phosphate in the 10% CO₂ group were significantly higher than those in the 0.038% CO₂ group. Correlation analysis showed that specific growth rate, carbon fixation rate, protein were all positively correlated with C. vulgaris biomass(R²>0.86, P<0.05). With 10% CO₂ and 60 mg/L NH₄Cl, the maximum values of specific growth rate, carbon fixation rate, and protein were 0.21 d^-1, 42.62 mg/(L·d), and 228.43 mg/L, respectively. In addition, the fitting results indicated that the combination of Logistic function and the modified Monod function could well describe C. vulgaris growth(correlation coefficient R² is 0.39~0.96), and nutrients were more easily utilized by C. vulgaris at 10% CO₂. This experiment could provide a theoretical reference for fixing CO₂, purifying wastewater and producing protein simultaneously by microalgae.
- Chlorella vulgaris,
- CO₂ fixation,
- ammonium,
- phosphate,
- the modified Monod function

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References(44)

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