BIOAUGMENTATION REMEDIATION OF AGEING OIL-CONTAMINATED SOIL IN DECOMMISSIONED WELL FIELD

DING Ming-shan; LIU Jing-xuan; SUN Nan; YUAN Zhang-zhong; WANG Wei-dong; LI Xiao-dong; WANG Zhi-jian

doi:10.13205/j.hjgc.202205023

Volume 40 Issue 5

Jul. 2022

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2022 > 40(5): 159-165

DING Ming-shan, LIU Jing-xuan, SUN Nan, YUAN Zhang-zhong, WANG Wei-dong, LI Xiao-dong, WANG Zhi-jian. BIOAUGMENTATION REMEDIATION OF AGEING OIL-CONTAMINATED SOIL IN DECOMMISSIONED WELL FIELD[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(5): 159-165. doi: 10.13205/j.hjgc.202205023

Citation:

DING Ming-shan, LIU Jing-xuan, SUN Nan, YUAN Zhang-zhong, WANG Wei-dong, LI Xiao-dong, WANG Zhi-jian. BIOAUGMENTATION REMEDIATION OF AGEING OIL-CONTAMINATED SOIL IN DECOMMISSIONED WELL FIELD[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(5): 159-165. doi: 10.13205/j.hjgc.202205023

Citation:

DING Ming-shan, LIU Jing-xuan, SUN Nan, YUAN Zhang-zhong, WANG Wei-dong, LI Xiao-dong, WANG Zhi-jian. BIOAUGMENTATION REMEDIATION OF AGEING OIL-CONTAMINATED SOIL IN DECOMMISSIONED WELL FIELD[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(5): 159-165. doi: 10.13205/j.hjgc.202205023

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BIOAUGMENTATION REMEDIATION OF AGEING OIL-CONTAMINATED SOIL IN DECOMMISSIONED WELL FIELD

doi: 10.13205/j.hjgc.202205023

1. Sinopec Key Laboratory of Microbial Enhanced Oil Recovery, Dongying 257000, China;
2. Research Institute of Petroleum Engineering and Technology, Shengli Oilfield Company, Sinopec, Dongying 257000, China;
3. Sinopec Xinjiang Xinchun Petroleum Exploitation Co., Ltd., Tacheng 833099, China;
4. Gudong Oil Production Plant, Shengli Oilfield Company, Sinopec, Dongying 257237, China;
5. Jinan Hongfeng Chemical Co. Ltd., Jinan 251412, China

Received Date: 2021-08-16
Available Online: 2022-07-02

Abstract

Abstract

Oil contaminated soils from the decommissioned well site were with low oil content with an increasing heavy fraction over time. Therefore, bioaugmentation was performed to restore the original properties of the aging contaminated soil. The physicochemical properties and indigenous microbial communities of the contaminated soil from a decommissioned well site were systematically analyzed. Various hydrocarbon degrading strains and bio-emulsifiers were screened to promote a high degradation value. Moreover, the preparation method of immobilized strains was optimized to enhance the environmental resistance. It was found that the oil content of the contaminated soils was up to 14.6 mg/g with 57% of heavy component. There was a lack of heavy oil degrading microorganisms in the soils. Two functional degrading strains with synergistic effects were screened and immobilized to improve the efficiency of hydrocarbon degradation. With the addition of 500 mg/L F-3 bio-emulsifier, the degradation time of meeting the standard was reduced from 120 d to 80 d. Thereafter, a 2700 m² field test was carried out with the land farming method. After 8 months, the oil content was reduced from 14.6 mg/g to 3.30 mg/g, meeting the requirements of state environmental standards, GB 36600-2018. The screened strains were capable of increasing the degradation rate of the aging oil contaminated soils, where the optimized emulsifier and adding method were in favor of reducing the remediation period.
- ageing oil contaminated soil,
- bioaugmentation,
- hydrocarbon-degrading bacteria,
- indigenous microbial communities,
- field test

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References(31)

References

[1]	BALBA M T,AL-AWADHI N,AL-DAHER R.Bioremediation of oil-contaminated soil:microbiological methods for feasibility assessment and field evaluation[J].Journal of Microbiological Methods,1998,32(2):155-164.
[2]	张强,傅晓文,季蕾,等.生态堆技术修复老化油泥污染土壤[J].环境工程学报,2017,11(11):6142-6146.
[3]	GONG X B.Remediation of weathered petroleum oil-contaminated soil using a combination of biostimulation and modified Fenton oxidation[J].International Biodeterioration& Biodegradation,2012,70:89-95.
[4]	赵晓非,张晓阳,刘立新,等.新型油泥处理技术展望[J].化工进展,2016,35(增刊1):276-280.
[5]	高梦雯.高浓度有机污染土壤处理技术研究进展[J].环境与发展,2019,31(3):41-42.
[6]	BOOPATHY R.Factors limiting bioremediation technologies[J].Bioresource Technology,2000,74(1):63-67.
[7]	SHARMA I.Bioremediation Techniques for Polluted Environment:Concept,Advantages,Limitations,and Prospects[M].Trace Metals in the Environment-New Approaches and Recent Advances,Intecopen,2021,2:2-5.
[8]	董丁,卢彦珍,唐美华,等.一株石油烃高效降解菌的筛选及降解性能研究[J].南京工业大学学报(自然科学版),2017,39(3):6.
[9]	FATIMA M B,FLÁVIO A C,BENEDICT C O,et al.Comparative bioremediation of soils contaminated with diesel oil by natural attenuation,biostimulation and bioaugmentation[J].Bioresource Technology,2005,96(9):1049-1055.
[10]	张宝良,王宝辉,田禹,等.油污土壤生物修复高效菌筛选鉴定[J].哈尔滨工业大学学报,2006,38(12):2180-2184.
[11]	UENO A,HASANUZZAMAN M,YUMOTO I,et al.Verification of degradation of n-alkanes in diesel oil by Pseudomonas aeruginosa strain watg in soil microcosms[J].Current Microbiology,2006,52(3):182-185.
[12]	BANET G,TURAANI A K,FARBER R,et al.The effects of biostimulation and bioaugmentation on crude oil biodegradation in two adjacent terrestrial oil spills of different age,in a hyper-arid region[J].Journal of Environmental Management,2021,286(3/4):112248.
[13]	VARJAIVI S,UPASANI V N.Bioaugmentation of Pseudomonas aeruginosa NCIM 5514-A novel oily waste degrader for treatment of petroleum hydrocarbons[J].Bioresource Technology,2020,319:124240.
[14]	王丽萍,李丹,许锐伟,等.专性菌系对石油烃污染土壤的修复性能[J].中国环境科学,2018,38(4):1417-1423.
[15]	李斌,张随望,黎成,等.安塞油田含油污泥井场微生物降解处理技术研究[J].石油天然气学报,2009,31(3):354-356.
[16]	代小丽,王硕,李佳斌,等.石油污染土壤原位生物修复强化技术研究进展[J].环境工程技术学报,2020,10(3):456-466.
[17]	蔡萍萍,宁卓,何泽,等.采油井场土壤微生物群落结构分布[J].环境科学,2018,39(7):3329-3338.
[18]	DING M S,JIA W H,LV Z F,et al.Improving bitumen recovery from poor processing oil sands using microbial pretreatment[J].Energy& Fuels,2014,28(11):7712-7720.
[19]	胡婧,束青林,孙刚正,等.油藏内源微生物演替规律及其对驱油效果的影响[J].中国石油大学学报(自然科学版),2019,43(1):108-114.
[20]	慕庆峰,于立红,张涛,等.油污土壤修复微生物的筛选及其影响因素[J].水土保持通报,2018,38(5):330-346.
[21]	MOSLEM A,MEHDI H,AKRAMSADAT E.Degradation of phenol at high concentrations using immobilization Pseudomonas putida P53 into sawdust entrapped into sodium-alginate beads[J].Water Science& Technology,2019,79(7):1387-1396.
[22]	孙万虹,陈丽华,徐红伟.氮磷含量对微生物修复油污土壤的影响[J].生物技术通报,2015,31(6):157-164.
[23]	VIDALI M.Bioremediation.An overview[J].Pure& Applied Chemistry,2001,73(7):1163-1172.
[24]	MINAI-TEHRANI D,ROHANIFAR P,AZAMI S.Assessment of bioremediation of aliphatic,aromatic,resin,and asphaltene fractions of oil-sludge-contaminated soil[J].International Journal of Environmental Science& Technology,2015,12(4):1253-1260.
[25]	SIMONE C,RENATA D,MARIA G,et al.Predominant growth of Alcanivorax during experiments on "oil spill bioremediation" in mesocosms[J].Microbiological Research,2007,162(2):185-190.
[26]	SARBASHIS,DAS B M,FREDRIK P,et al.Characterization of three mycobacteriumspp.with potential use in bioremediation by genome sequencing and comparative genomics[J].Genome Biology& Evolution,2015,7(7):1870-1885.
[27]	YUMIKO K,LIES I S,ATSUKO U,et al.Thalassospira tepidiphila sp.nov.,a polycyclic aromatic hydrocarbon-degrading bacterium isolated from seawater[J].International Journal of Systematic& Evolutionary Microbiology,2008,58(3):711-715.
[28]	李晓楼.石油污染对土壤微生物群落多样性的影响[J].生物工程学报,2017,33(6):968-975.
[29]	ELLEN C S,THEREZA C VP,RICARDO P O.Biosurfactant-enhanced hydrocarbon bioremediation:an overview[J].International Biodeterioration& Biodegradation,2014,89(2):88-94.
[30]	张秀霞,耿春香,房苗苗,等.固定化微生物应用于生物修复石油污染土壤[J].石油学报(石油加工),2008,24(4):409-414.
[31]	王京秀,张志勇,孙珊珊,等.植物-固体菌剂联合修复石油污染土壤的基础研究[J].环境工程学报,2016,10(11):6732-6738.