ENHANCED METHANOGENESIS OF WASTE ACTIVATED SLUDGE FERMENTATION BY DOSING AN ALGINATE-DEGRADING CONSORTIUM

GENG Ziqian; DAI Wenting; LI Chao; TANG Jie; DAI Kun; CENG Jianxiong; ZHANG Fang

doi:10.13205/j.hjgc.202208012

Volume 40 Issue 8

Nov. 2022

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2022 > 40(8): 90-95

MOU Zijie, LI Junqi, LI Xiaojing. RESEARCH PROGRESS ON APPLICATION OF GEOSYNTHETICS IN GREEN STORMWATER INFRASTRUCTURE[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(8): 206-212. doi: 10.13205/j.hjgc.202208029

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GENG Ziqian, DAI Wenting, LI Chao, TANG Jie, DAI Kun, CENG Jianxiong, ZHANG Fang. ENHANCED METHANOGENESIS OF WASTE ACTIVATED SLUDGE FERMENTATION BY DOSING AN ALGINATE-DEGRADING CONSORTIUM[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(8): 90-95. doi: 10.13205/j.hjgc.202208012

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ENHANCED METHANOGENESIS OF WASTE ACTIVATED SLUDGE FERMENTATION BY DOSING AN ALGINATE-DEGRADING CONSORTIUM

doi: 10.13205/j.hjgc.202208012

College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Received Date: 2021-10-01
Publish Date: 2022-11-08

Abstract

Abstract

Methane production by anaerobic digestion is popular biotechnology to utilize the wanted waste activated sludge (WAS). But the hydrolysis is known as the rate-limiting step of WAS fermentation. The uronic acids (such as alginate and polygalacturonic acid) in WAS can form hydrogels with cationic ions (i.e. Ca²⁺) in wastewater, which can maintain sludge structure and retard the microbial hydrolysis. Thus, these organics are deemed as the main components of structural extracellular polymeric substances. In this work, an alginate-degrading consortium (ADC) with high activity was enriched in a mesophilic chemostat using alginate as the substrate. The results showed that the enriched ADC could notably enhance methane production from WAS. The methane yield increased by 53.6% under a low ratio of inoculum to WAS of 0.03 g/g [m(ADC)∶m(VSS)]. Moreover, the typical organic components of WAS, including polygalacturonic acid, dextran, and casein, could be utilized by the enriched ADC. The genus Bacteroides (96.3%) was identified as the dominant bacteria in ADC by an Illumina Miseq high-throughput sequencing. Therefore, this work provided a new microbial method for biochemical production in WAS.
- alginate-degrading consortium,
- waste activated sludge,
- anaerobic digestion,
- methane,
- genus Bacteroides

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References

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