DENITRIFICATION ENHANCEMENT EFFECT AND MICROBIAL FLORA ANALYSIS OF A NEW BIOMASS CARBON SOURCE

WANG Yan; LI Ji; ZHI Yao; ZHOU Yu; ZHENG Kai-kai; WANG Xiao-fei

doi:10.13205/j.hjgc.202209008

Volume 40 Issue 9

Nov. 2022

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WANG Yan, LI Ji, ZHI Yao, ZHOU Yu, ZHENG Kai-kai, WANG Xiao-fei. DENITRIFICATION ENHANCEMENT EFFECT AND MICROBIAL FLORA ANALYSIS OF A NEW BIOMASS CARBON SOURCE[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(9): 63-68,117. doi: 10.13205/j.hjgc.202209008

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WANG Yan, LI Ji, ZHI Yao, ZHOU Yu, ZHENG Kai-kai, WANG Xiao-fei. DENITRIFICATION ENHANCEMENT EFFECT AND MICROBIAL FLORA ANALYSIS OF A NEW BIOMASS CARBON SOURCE[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(9): 63-68,117. doi: 10.13205/j.hjgc.202209008

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DENITRIFICATION ENHANCEMENT EFFECT AND MICROBIAL FLORA ANALYSIS OF A NEW BIOMASS CARBON SOURCE

doi: 10.13205/j.hjgc.202209008

WANG Yan^1,2,3,
LI Ji^{1,4,5
,
,},
ZHI Yao^1,3,
ZHOU Yu⁶,
ZHENG Kai-kai^1,3,
WANG Xiao-fei^1,3

1. School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China;
2. Jiangsu GL Technology Co., Ltd, Wuxi 214106, China;
3. Wuxi Puhui Environmental Technology Co., Ltd, Wuxi 214028, China;
4. Jiangsu Key Laboratory of Anaerobic Biotechnology, Jiangnan University, Wuxi 214122, China;
5. Jiangsu College Water Treatment Technology and Material Collaborative Innovation Center, Suzhou 215009, China;
6. Wuxi Water Group Co., Ltd, Wuxi 214031, China

Received Date: 2021-12-20
Available Online: 2022-11-09

Abstract

Abstract

To achieve the deep denitrification effect of wastewater treatment plants, taking a sewage treatment plant in Taihu Lake Basin as an example, the biomass carbon source with derivatives of biomass waste as the main raw material was used as the electron donor for denitrification, and the denitrification effects of the anoxic tank and deep-bed filter were studied respectively. The changes in microbial community structure before and after the addition of carbon sources were also analyzed. The results showed that 1.67~1.73 mg/L nitrate could be removed, when the biomass carbon source dosage was 2.5~3.0 t/d, and the removal rate was about 52%~68%. Meanwhile, the deep bed filter consumed about 5.27 mg COD for every 1 mg NO₃^--N removal after adding biomass carbon source in the process of denitrification. Thus, the effluent TN was stable and met the discharge limit requirements of class Ⅰ and Ⅱ protected areas in the Discharge Standard of Main Water Pollutants for Municipal Wastewater Treatment Plant & Key Industries in Taihu Area(DB 32/1072—2018). Through 16 S rRNA gene sequence analysis, it was found that the dominant microbial phyla of hypoxic pools and deep-bed filters were Proteobacteria, Actinobacteria, Chloroflexi, and Bacteroidota. After adding biomass carbon source to deep-bed filter with different process conditions and growth environment, the dominant flora with denitrification function such as Thiothrix, Bacillus, Propionicicella, norank_f_Rhodocyclaceae and Terrimonas were more prominent and further effectively ensured the stable deep nitrogen removal performance of the system. At the same time, the adding of biomass carbon source indirectly reduced carbon dioxide emissions, which made a positive contribution to carbon emission reduction and carbon neutralization.
- biomass carbon source,
- denitrification,
- nitrogen removal,
- 16S rRNA,
- community structure

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References(28)

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