EFFECT OF SULFAMETHOXAZOLE ON NITROGEN REMOVAL AND MICROBIAL COMMUNITY OF SEQUENCING BATCH BIOREACTORS

YAN Duosen; YANG Wen; LI Shanshan; JIAO Yan; ZHANG Guodong; CHEN Qinghua; LI Yun

doi:10.13205/j.hjgc.202210003

Volume 40 Issue 10

Oct. 2022

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YAN Duosen, YANG Wen, LI Shanshan, JIAO Yan, ZHANG Guodong, CHEN Qinghua, LI Yun. EFFECT OF SULFAMETHOXAZOLE ON NITROGEN REMOVAL AND MICROBIAL COMMUNITY OF SEQUENCING BATCH BIOREACTORS[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(10): 15-23,70. doi: 10.13205/j.hjgc.202210003

Citation:

YAN Duosen, YANG Wen, LI Shanshan, JIAO Yan, ZHANG Guodong, CHEN Qinghua, LI Yun. EFFECT OF SULFAMETHOXAZOLE ON NITROGEN REMOVAL AND MICROBIAL COMMUNITY OF SEQUENCING BATCH BIOREACTORS[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(10): 15-23,70. doi: 10.13205/j.hjgc.202210003

Citation:

YAN Duosen, YANG Wen, LI Shanshan, JIAO Yan, ZHANG Guodong, CHEN Qinghua, LI Yun. EFFECT OF SULFAMETHOXAZOLE ON NITROGEN REMOVAL AND MICROBIAL COMMUNITY OF SEQUENCING BATCH BIOREACTORS[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(10): 15-23,70. doi: 10.13205/j.hjgc.202210003

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EFFECT OF SULFAMETHOXAZOLE ON NITROGEN REMOVAL AND MICROBIAL COMMUNITY OF SEQUENCING BATCH BIOREACTORS

doi: 10.13205/j.hjgc.202210003

College of Resources and Environment, Qingdao Agricultural University, Qingdao 266109, China

Received Date: 2022-02-07

Abstract

Abstract

The effects of long-term exposure to sulfamethoxazole (SMX) on nitrogen removal performance and microbial community in sequence batch bioreactor (SBR) were studied. The results showed that the removal effect of organic matter and NH₄⁺-N by SBR didn't change significantly after long-term exposure to a 6 mg/L SMX environment. The average removal rate of COD was (92.57±0.92)%, and that of NH₄⁺-N reached (98.71±0.34)%. The effluent concentrations of NO₂^--N and NO₃^--N increased with the increase in operation time. On the 104th day, the specific oxygen consumption rate (SOUR), specific ammonia oxidation rate (SAOR), specific nitrate oxidation rate (SNOR), specific nitrate reduction rate (SNRR), and specific nitrite reduction rate (SNIRR) decreased by 23.33%, 24.47%, 28.29%, 14.97% and 15.81%, compared with those before adding SMX. With the existence of SMX, the contents of extracellular polymeric substance (EPS), loosely bound extracellular polymeric substance (LB-EPS) and tightly bound extracellular polymeric substance (TB-EPS), protein (PN) and polysaccharide (PS) increased with the increase of operation time, and PN/PS showed an upward trend. The production of reactive oxygen species (ROS) and lactate dehydrogenase (LDH) increased with the increase of running time. 6 mg/L SMX could reduce the richness and increase the diversity of the microbial community in activated sludge, and decrease the relative abundance of Proteobacteria, Chloroflexi, and Gammaproteobacteria, thus affecting the nitrogen removal performance of SBR.
- sulfamethoxazole,
- activated sludge,
- denitrification rate,
- extracellular polymer,
- microbial community

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