ENHANCEMENT OF COMPOSITE SOLID CARBON SOURCES ON NITROGEN REMOVAL PERFORMANCE OF A RURAL DOMESTIC SEWAGE TREATMENT PROCESS AND FUNCTIONAL BACTERIAL COMMUNITY

JIANG Yanbo; LING Wei; WEI Chunzhong; WEI Yuan; WU Chunfeng; WANG Chao; MENG Fangang

doi:10.13205/j.hjgc.202210009

Volume 40 Issue 10

Oct. 2022

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JIANG Yanbo, LING Wei, WEI Chunzhong, WEI Yuan, WU Chunfeng, WANG Chao, MENG Fangang. ENHANCEMENT OF COMPOSITE SOLID CARBON SOURCES ON NITROGEN REMOVAL PERFORMANCE OF A RURAL DOMESTIC SEWAGE TREATMENT PROCESS AND FUNCTIONAL BACTERIAL COMMUNITY[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(10): 62-70. doi: 10.13205/j.hjgc.202210009

Citation:

JIANG Yanbo, LING Wei, WEI Chunzhong, WEI Yuan, WU Chunfeng, WANG Chao, MENG Fangang. ENHANCEMENT OF COMPOSITE SOLID CARBON SOURCES ON NITROGEN REMOVAL PERFORMANCE OF A RURAL DOMESTIC SEWAGE TREATMENT PROCESS AND FUNCTIONAL BACTERIAL COMMUNITY[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(10): 62-70. doi: 10.13205/j.hjgc.202210009

Citation:

JIANG Yanbo, LING Wei, WEI Chunzhong, WEI Yuan, WU Chunfeng, WANG Chao, MENG Fangang. ENHANCEMENT OF COMPOSITE SOLID CARBON SOURCES ON NITROGEN REMOVAL PERFORMANCE OF A RURAL DOMESTIC SEWAGE TREATMENT PROCESS AND FUNCTIONAL BACTERIAL COMMUNITY[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(10): 62-70. doi: 10.13205/j.hjgc.202210009

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ENHANCEMENT OF COMPOSITE SOLID CARBON SOURCES ON NITROGEN REMOVAL PERFORMANCE OF A RURAL DOMESTIC SEWAGE TREATMENT PROCESS AND FUNCTIONAL BACTERIAL COMMUNITY

doi: 10.13205/j.hjgc.202210009

1. School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China;
2. Guangxi Beitou Environmental Protection & Water Group Co., Ltd, Nanning 530029, China

Received Date: 2021-10-28

Abstract

Abstract

For the low carbon nitrogen ratio (C/N) of rural domestic sewage limited the denitrification course, a sequencing batch reactor (SBR) process enhanced by composite solid carbon sources (SCS-SBR) was used to improve the treatment efficiency of rural domestic sewage (compost of water diversion project sewage and village sewage). The unique functional bacterial community was also thoroughly examined during the operation of SCS-SBR. The denitrification capacity of the SBR process could be effectively improved by incorporating composite solid carbon sources (PHBV+straw). The experimental results showed that the effluent COD, NH₄⁺-N and TN concentrations remained below 25.0, 0.4, and 5.0 mg/L throughout the stable operation of the SCS-SBR process, respectively, and TN removal efficiency of village sewage reached 83.1%. Following the addition of SCS to SBR, Illumina sequencing analysis revealed that some unique functional bacteria were screened out. In contrast to the nitrifying bacteria (Nitrosomonas) and the denitrifying bacteria (Pseudomonas) in the traditional activated sludge, the nitrifying bacteria in the SCS-SBR process was norank_f_JG30-KF-CM45, and the denitrifying functional bacteria were Thermomonas and Rubrivax. The relative abundance of Thermomonas increased from undetectable in the stage without solid carbon sources (AS1 and AS2), to 2.54% and 7.55% in the stage with solid carbon sources (SCS1 and SCS2), respectively. Furthermore, the relative abundance of an aerobic and oxygen stress-tolerant bacteria in the activated sludge, Nakamurella decreased dramatically from 44.52% and 57.66% in AS1 and AS2, to 1.06% and 0.86% in SCS1 and SCS2, respectively, indicating that microorganisms that cannot utilize solid carbon sources in the process were gradually eliminated. In conclusion, composite solid carbon sources (PHBV+straw) could eliminate the flaws of liquid carbon sources, improve the denitrification capacity of the SBR process and efficiently screen the functional microorganisms in the system. This study provides a theoretical basis and technical support for rural domestic sewage treatment.
- rural domestic sewage,
- low carbon nitrogen ratio,
- composite solid carbon sources,
- SCS-SBR process,
- functional bacterial community

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