ACCELERATION OF VARIOUS ALKANES BALANCED DEGRADATION BY SOIL MICROORGANISMS WITH FENTON PRE-OXIDATION

XU Jinlan; TIAN Guiyong; SHI Qihang

doi:10.13205/j.hjgc.202302018

Volume 41 Issue 2

Feb. 2023

Turn off MathJax

Article Contents

Article Navigation > ENVIRONMENTAL ENGINEERING > 2023 > 41(2): 131-139

XU Jinlan, TIAN Guiyong, SHI Qihang. ACCELERATION OF VARIOUS ALKANES BALANCED DEGRADATION BY SOIL MICROORGANISMS WITH FENTON PRE-OXIDATION[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(2): 131-139. doi: 10.13205/j.hjgc.202302018

Citation:

XU Jinlan, TIAN Guiyong, SHI Qihang. ACCELERATION OF VARIOUS ALKANES BALANCED DEGRADATION BY SOIL MICROORGANISMS WITH FENTON PRE-OXIDATION[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(2): 131-139. doi: 10.13205/j.hjgc.202302018

Citation:

PDF( 1469 KB)

ACCELERATION OF VARIOUS ALKANES BALANCED DEGRADATION BY SOIL MICROORGANISMS WITH FENTON PRE-OXIDATION

doi: 10.13205/j.hjgc.202302018

XU Jinlan^{1,2
,
,},
TIAN Guiyong^1,2,
SHI Qihang^1,2

1. School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China;
2. Shaanxi Key Laboratory of Environmental Engineering, Xi'an 710055, China

Received Date: 2022-04-11
Available Online: 2023-05-25
Publish Date: 2023-02-01

Abstract

Abstract

Aiming at selective biodegradation of various alkanes in the petroleum contaminated soil, microbial communities in the soil were screened by regulating multiple soil solid phase Fe Fenton pre-oxidation. Then, the influence of soil microbial quantity, activity and community changes on the degradation of petroleum hydrocarbons was explored, and the microbial community characteristics for the balanced degradation of various alkanes were determined. The results showed that:after Fenton pre-oxidation of solid phase Fe with A45 (45 mmol/L citric acid) and F8.7 (8.7 mmol/L Fe²⁺), the metabolic activity of soil microorganisms was as high as 0.59 mol/kg (A45) and 0.60 mol/kg (F8.7), respectively. The petroleum hydrocarbon residual rate in the soil was reduced to 30% (A45) and 29% (F8.7), respectively. At the same time, there were soil microbial communities containing Acinetobacter, and Pseudomonas as dominant bacterial strains in the soil. The diversity of soil microorganisms was high, the community composition was rich, and the abundance of functional genes in alkane metabolism was high. Based on this situation, the balanced degradation of various alkanes was realized and promoted, and the biodegradation of various alkanes in the soil all reached 60%.
- alkane,
- microbial community,
- Fenton pre-oxidation,
- high-throughput sequencing,
- petroleum contaminated soil

FullText(HTML)

References(51)

References

[1]	郝春博, 王广才, 董健楠, 等. 石油污染地下水中细菌多样性研究[J]. 环境科学, 2009, 30(8):2464-2472.
[2]	郑红婷, 张秀霞, 钟哲森,等. 漆酶修复石油污染土壤优化实验[J]. 现代化工, 2017, 37(4):109-112.
[3]	STRECHE C, COCARTA D M, ISTRATE I A, et al. Decontamination of petroleum-contaminated soils using the electrochemical technique:remediation degree and energy consumption[J]. Scientific Reports, 2018, 8:3272.
[4]	曾琪静, 丁丽, 文方, 等. 优化过硫酸盐体系处理石油类污染土壤[J]. 环境工程, 2019, 37(2):170-174.
[5]	O'BRIEN P L, DESUTTER T M, CASEY F X M, et al. Evaluation of soil function following remediation of petroleum hydrocarbons:a review of current remediation techniques[J]. Current Pollution Reports, 2017, 3:192-205.
[6]	LEE D W, LEE H, KWON B O, et al. Biosurfactant-assisted bioremediation of crude oil by indigenous bacteria isolated from Taean beach sediment[J]. Environmental Pollution, 2018, 241:254-264.
[7]	孙秦川, 王宝山, 谭磊, 等. 青藏高原土壤柴油降解菌的筛选及降解实验研究[J]. 环境工程, 2017, 35(5):167-171.
[8]	XU J L, WANG J, WANG C, et al. Effective oxidation of crude oil in soils by consuming less hydroxyl radical with target iron[J]. Chemical Engineering Journal, 2020, 380:122414.
[9]	SIMPANEN S, DAHL M, GERLACH M, et al. Biostimulation proved to be the most efficient method in the comparison of in situ soil remediation treatments after a simulated oil spill accident[J]. Environmental Science and Pollution Research, 2016, 23(24):25024-25038.
[10]	MARIANO A P, KATAOKA A P D A, ANGELIS D D F D, et al. Laboratory study on the bioremediation of diesel oil contaminated soil from a petrol station[J]. Brazilian Journal of Microbiology, 2007, 38(2):346-353.
[11]	樊鑫, 刘璐. 生物修复技术在石油污染治理中的应用研究进展[J]. 现代化工, 2021, 41(12):64-68.
[12]	田西昭, 袁子婷, 宫志强, 等. 复配微生物菌群修复石油污染土壤的实验研究[J]. 环境科学与技术, 2020, 43(增刊2):74-78.
[13]	CHEN Y, LI C, ZHOU Z X, et al. Enhanced biodegradation of alkane hydrocarbons and crude oil by mixed strains and bacterial community analysis[J]. Applied Biochemistry & Biotechnology, 2014, 172:3433-3447.
[14]	ZHANG X Y, KONG D W, LIU X Y, et al. Combined microbial degradation of crude oil under alkaline conditions by Acinetobacter baumannii and Talaromyces sp[J]. Chemosphere, 2021, 273:129666.
[15]	田秀梅, 王晓丽, 彭士涛, 等. 一株高效原油降解不动杆菌的筛选及降解特性分析[J]. 环境工程, 2019, 37(6):165-169 ,95.
[16]	张辉, 李培军, 王桂燕, 等. 固定化混合菌修复油污染地表水的研究[J]. 环境工程学报, 2008,2(12):1613-1617.
[17]	王志强, 武强, 叶思源, 等. 地下水石油污染高效生物降解研究[J]. 环境科学, 2005,26(6):63-66.
[18]	GHORBANNEZHAND H, MOGHIMI H, DASTGHEIB S M M. Evaluation of heavy petroleum degradation using bacterial-fungal mixed cultures[J]. Ecotoxicology and Environmental Safety, 2018, 164:434-439.
[19]	周海霞, 单爱琴, 王莉淋, 等. 石油降解菌的筛选及其降解效率的研究[J]. 环境科学与技术, 2008, 31(10):56-58.
[20]	XU J L, FAN X S, HUANG F D, et al. Iron bound to soil organic matter catalyzes H₂O₂ to oxidize crude oil in soil[J]. Journal of Hazardous Materials, 2017, 322:516-524.
[21]	郑园园. 激活嗜油微生物高效修复重度原油污染土壤的实验研究[D]. 西安:西安建筑科技大学, 2021.
[22]	XU J L, XIN L, HUANG T L, et al. Enhanced bioremediation of oil contaminated soil by graded modified Fenton oxidation[J]. Journal of Environmental Sciences, 2011, 23(11):1873-1879.
[23]	曹倩倩. 石油污染土壤的固相铁Fenton氧化优化试验研究[D]. 西安:西安建筑科技大学, 2018.
[24]	JHO E H, RYU H, SHIN D, et al. Prediction of landfarming period using degradation kinetics of petroleum hydrocarbons:test with artificially contaminated and field-aged soils and commercially available bacterial cultures[J]. Journal of Soils and Sediments, 2014, 14(1):138-145.
[25]	HAMZAH A, PHAN C W, BAKAR N F A, et al. Biodegradation of crude oil by constructed bacterial consortia and the constituent single bacteria isolated from malaysia[J]. Bioremediation Journal, 2013, 17(1):1-10.
[26]	SUTTON N B, GROTENHUIS T, RIJNAARTS H H. Impact of organic carbon and nutrients mobilized during chemical oxidation on subsequent bioremediation of a diesel-contaminated soil[J]. Chemosphere, 2014, 97:64-70.
[27]	XU J L, ZHANG Q J, LI D Y, et al. Rapid degradation of long-chain crude oil in soil by indigenous bacteria using fermented food waste supernatant[J]. Waste Management, 2019, 85:361-373.
[28]	刘小为, 陈忠林, 沈吉敏, 等. 硫酸钛光度法测定O₃/H₂O₂体系中低浓度H₂O₂[J]. 中国给水排水, 2010, 26(16):126-129.
[29]	MOHANTY G, MUKHERJI S. Biodegradation rate of diesel range n-alkanes by bacterial cultures Exiguobacterium aurantiacum and Burkholderia cepacia[J]. International Biodeterioration & Biodegradation, 2008, 61(3):240-250.
[30]	贾凌慧, 郑世浩, 孙丽慧, 等. 石油降解低温细菌的筛选及降解特性的表征[J]. 环境工程, 2020, 38(6):252-258.
[31]	XU J L, DU J, LI L, et al. Fast-stimulating bioremediation of macro crude oil in soils using matching Fenton pre-oxidation[J]. Chemosphere, 2020, 252:126622.
[32]	BAJAGAIN R, PARK Y, JEONG S W. Feasibility of oxidation-biodegradation serial foam spraying for total petroleum hydrocarbon removal without soil disturbance[J]. Science of the Total Environment, 2018, 626:1236-1242.
[33]	GONG X B. Remediation of weathered petroleum oil-contaminated soil using a combination of biostimulation and modified Fenton oxidation[J]. International Biodeterioration & Biodegradation, 2012, 70:89-95.
[34]	XU J L, DENG X, CUI Y W, et al. Impact of chemical oxidation on indigenous bacteria and mobilization of nutrients and subsequent bioremediation of crude oil-contaminated soil[J]. Journal of Hazardous Materials, 2016, 320:160-168.
[35]	SANDU C, POPESCU M, ROSALES E, et al. Electrokinetic-Fenton technology for the remediation of hydrocarbons historically polluted sites[J]. Chemosphere, 2016, 156:347-356.
[36]	BAJAGAIN R, LEE S, JEONG S W. Application of persulfate-oxidation foam spraying as a bioremediation pretreatment for diesel oil-contaminated soil[J]. Chemosphere, 2018, 207:565-572.
[37]	FENG L Y, JIANG X P, HUANG Y N, et al. Petroleum hydrocarbon-contaminated soil bioremediation assisted by isolated bacterial consortium and sophorolipid[J]. Environmental Pollution, 2021, 273:116476.
[38]	LI X F, ZHAO L, ADAM M. Biodegradation of marine crude oil pollution using a salt-tolerant bacterial consortium isolated from Bohai Bay, China[J]. Marine Pollution Bulletin, 2016, 105(1):43-50.
[39]	DAI X L, LV J, YAN G X, et al. Bioremediation of intertidal zones polluted by heavy oil spilling using immobilized laccase-bacteria consortium[J]. Bioresource Technology, 2020, 309:123305.
[40]	NIE Y, LIANG J L, FANG H, et al. Characterization of a CYP153 alkane hydroxylase gene in a Gram-positive Dietzia sp. DQ12-45-1b and its "team role" with alkW1 in alkane degradation[J]. Applied Microbiology & Biotechnology, 2014, 98(1):163-173.
[41]	QIN G, GONG D, FAN M Y. Bioremediation of petroleum-contaminated soil by biostimulation amended with biochar[J]. International Biodeterioration & Biodegradation, 2013, 85:150-155.
[42]	姜睿玲, 杨统一, 唐玉斌, 等. 多环芳烃污染对桑园土壤微生物结构及种群多样性的影响[J]. 中国环境科学, 2012, 32(9):1655-1661.
[43]	张博凡, 熊鑫, 韩卓, 等. 菌糠强化微生物降解石油污染土壤修复研究[J]. 中国环境科学, 2019, 39(3):1139-1146.
[44]	郑瑾, 王馨妤, 李杰, 等. 腐植酸改性生物质电厂灰固定化微生物修复石油烃污染土壤[J]. 环境工程, 2020, 38(8):34-40.
[45]	徐苗, 段魏魏, 赵亚光, 等. 石油污染土壤理化性质对微生物代谢特征的影响[J]. 环境工程, 2018, 36(2):178-183.
[46]	吴蔓莉, 陈凯丽, 叶茜琼, 等. 堆肥-生物强化对重度石油污染土壤的修复作用[J]. 环境科学, 2017, 38(10):4412-4419.
[47]	吴蔓莉, 李可欣, 侯爽爽, 等. 贫养分低有机质黄绵土中石油烃的生物去除特性及菌群结构变化[J]. 环境科学研究, 2021, 34(8):1961-1970.
[48]	邓春萍, 龚汉意, 杜国勇, 等. 重复接种菌群强化修复石油污染土壤[J]. 环境科学与技术, 2021, 44(9):127-134.
[49]	王佳楠, 石妍云, 郑力燕, 等. 石油降解菌的分离鉴定及4株芽孢杆菌种间效应[J]. 环境科学, 2015, 36(6):2245-2251.
[50]	TIWARI B, MANICKAM N, KUMARI S, et al. Biodegradation and dissolution of polyaromatic hydrocarbons by Stenotrophomonas sp[J]. Bioresource Technology, 2016, 216:1102-1105.
[51]	荆佳维, 王卅, 郭书海. 典型油田区油污土壤微生物群落区域性分布研究[J]. 环境科学学报, 2021, 41(11):4660-4675.