EFFECTS OF TEMPERATURE ON ABUNDANCE AND POTENTIAL HOST OF ARGs DURING SLUDGE COMPOSTING

RUAN Min; WU Xikai; YANG Zhaohui; HUANG Jing; HUANG Zhongliang; LI Hui; WU Zijian; ZHANG Xuan; QIN Xiaoli; ZHANG Yanru

doi:10.13205/j.hjgc.202305011

Volume 41 Issue 5

May 2023

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2023 > 41(5): 75-83

RUAN Min, WU Xikai, YANG Zhaohui, HUANG Jing, HUANG Zhongliang, LI Hui, WU Zijian, ZHANG Xuan, QIN Xiaoli, ZHANG Yanru. EFFECTS OF TEMPERATURE ON ABUNDANCE AND POTENTIAL HOST OF ARGs DURING SLUDGE COMPOSTING[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(5): 75-83. doi: 10.13205/j.hjgc.202305011

Citation:

RUAN Min, WU Xikai, YANG Zhaohui, HUANG Jing, HUANG Zhongliang, LI Hui, WU Zijian, ZHANG Xuan, QIN Xiaoli, ZHANG Yanru. EFFECTS OF TEMPERATURE ON ABUNDANCE AND POTENTIAL HOST OF ARGs DURING SLUDGE COMPOSTING[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(5): 75-83. doi: 10.13205/j.hjgc.202305011

Citation:

RUAN Min, WU Xikai, YANG Zhaohui, HUANG Jing, HUANG Zhongliang, LI Hui, WU Zijian, ZHANG Xuan, QIN Xiaoli, ZHANG Yanru. EFFECTS OF TEMPERATURE ON ABUNDANCE AND POTENTIAL HOST OF ARGs DURING SLUDGE COMPOSTING[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(5): 75-83. doi: 10.13205/j.hjgc.202305011

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EFFECTS OF TEMPERATURE ON ABUNDANCE AND POTENTIAL HOST OF ARGs DURING SLUDGE COMPOSTING

doi: 10.13205/j.hjgc.202305011

1. School of Energy and Power Engineering, Changsha University of Science & Technology, Changsha 410076, China;
2. State Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410004, China;
3. College of Environmental Science and Engineering, Hunan University, Changsha 410082, China

Received Date: 2022-02-11

Abstract

Abstract

In order to explore the impact of the two key temperatures, the initial temperature (about 50 ℃) and the maximum temperature (about 70 ℃), on the antibiotic resistance genes (ARGs) during the thermophilic period of sludge composting, a real-time temperature compensation reactor was used to set three temperature conditions (R1: room temperature; R2: 50 ℃; R3: 70 ℃) to analyze the changing laws of ARGs, mobile genetic elements (MGEs) and their potential host. The results showed that the relative abundance of ARGs and MGEs could be reduced under the three temperatures during the thermophilic phase. Among them, sul2, tetG, aphA1 and IntI1 were extremely sensitive to temperature changes and showed similar removal rates, but the removal rates of tetM, mefA, sul1 and Tn916 were significantly different. In the cooling and maturity phase, R2 and R3 inhibited the recovery of the abundance of sul1, sul2, tetG and IntI1. In terms of bacterial community structure, R2 and R3 increased the relative abundance of Firmicutes during the thermophilic period, and decreased the relative abundance of Actinobacteria and Proteobacteria during the maturity phase. Co-occurrence network analysis showed that Proteobacteria, Firmicutes and Actinobacteria were the main potential hosts of target ARGs and MGEs. Meanwhile, horizontal gene transfer caused by IntI1 may be responsible for the rebound of sul1, sul2 and tetG during the cooling and maturity phase. This study can provide a reference for exploring the changing mechanism of ARGs at different sludge composting temperatures.
- aerobic composting,
- sludge,
- temperature,
- ARGs,
- potential host

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References(18)

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