DISSOLUTION AND EXTRACTION OF PROTEIN FROM SLUDGE VIA A COMBINATION OF ULTRASOUND AND DNA EXTRACTION PHENOL REAGENT ADDITION

CUI Jing; MIAO Fenghai; ZHANG Yun; ZHANG He; ZHOU Tao

doi:10.13205/j.hjgc.202306010

Volume 41 Issue 6

Jun. 2023

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2023 > 41(6): 71-75,91

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CUI Jing, MIAO Fenghai, ZHANG Yun, ZHANG He, ZHOU Tao. DISSOLUTION AND EXTRACTION OF PROTEIN FROM SLUDGE VIA A COMBINATION OF ULTRASOUND AND DNA EXTRACTION PHENOL REAGENT ADDITION[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(6): 71-75,91. doi: 10.13205/j.hjgc.202306010

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DISSOLUTION AND EXTRACTION OF PROTEIN FROM SLUDGE VIA A COMBINATION OF ULTRASOUND AND DNA EXTRACTION PHENOL REAGENT ADDITION

doi: 10.13205/j.hjgc.202306010

1. Tianjin Yuchuan Jinhong Environmental Protection Technology Co., Ltd., Tianjin 300459, China;
2. School of Environmental Science and Engineering, Tongji University, Shanghai 200092, China

Received Date: 2022-03-28
Available Online: 2023-09-02

Abstract

Abstract

Compared with the conventional treatment methods of sludge, the thermal alkali decomposition treatment was a preferred technology because of the high value-added protein products obtained. In this investigation, the mixture material from thermal alkali pretreatment was taken as the raw material, and variables including ultrasonic and chemical addition were investigated. Results showed that, the optimal ultrasonic power, time, initial temperature, pH and the ratio of phenol to DNA extraction reagent were 480 W, 20 min, 38℃, 7.83 and 6%, respectively. Under this condition, the protein dissolution reached 861.71 mg/L, 150% to 200% higher than the control groups. Correlation analysis suggested that except for the ultrasonic time, the effects of other factors on sludge protein concentration were extremely significant. Moreover, the addition of DNA extraction phenol reagent could desorb carbohydrates and proteins inside and outside the cell, and further increase the protein concentration to 861.71 mg/L in the filtrate with the 6% dosage.
- sludge,
- thermal alkaline hydrolysis,
- protein extraction,
- ultrasound,
- DNA extraction phenol reagent

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References(23)

References

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