MECHANISM OF CO-METABOLISM ENHANCEMENT OF EFFICIENT BIODEGRADATION OF PHENANTHRENE BY PHENOL

LI Shuang; ZHANG Aining; LIU Xingshe; YANG Lu; LIU Yongjun

doi:10.13205/j.hjgc.202307013

Volume 41 Issue 7

Jul. 2023

Turn off MathJax

Article Contents

Article Navigation > ENVIRONMENTAL ENGINEERING > 2023 > 41(7): 94-101

LI Shuang, ZHANG Aining, LIU Xingshe, YANG Lu, LIU Yongjun. MECHANISM OF CO-METABOLISM ENHANCEMENT OF EFFICIENT BIODEGRADATION OF PHENANTHRENE BY PHENOL[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(7): 94-101. doi: 10.13205/j.hjgc.202307013

Citation:

LI Shuang, ZHANG Aining, LIU Xingshe, YANG Lu, LIU Yongjun. MECHANISM OF CO-METABOLISM ENHANCEMENT OF EFFICIENT BIODEGRADATION OF PHENANTHRENE BY PHENOL[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(7): 94-101. doi: 10.13205/j.hjgc.202307013

Citation:

PDF( 2537 KB)

MECHANISM OF CO-METABOLISM ENHANCEMENT OF EFFICIENT BIODEGRADATION OF PHENANTHRENE BY PHENOL

doi: 10.13205/j.hjgc.202307013

LI Shuang^1,2,
ZHANG Aining^{1,2
,
,},
LIU Xingshe^1,2,
YANG Lu^1,2,
LIU Yongjun^1,2

1. School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China;
2. Key Laboratory of Northwest Water Resources, Environment and Ecology of Ministry of Education, Xi'an 710055, China

Received Date: 2022-08-15

Abstract

Abstract

A strain that could efficiently degrade phenanthrene was isolated from the aerobic sludge. Firstly, the degradation characteristics of phenanthrene with different carbon sources as co-substrates were explored. The results showed that the degradation rate of 30 mg/L phenanthrene reached 95.35% in the co-metabolism system with 80 mg/L phenol as a co-substrate.Compared with the non-co-metabolism system without phenol, the degradation rate of phenanthrene increased by 20.33%, and phenol was also mineralized. The co-metabolism enhancement mechanism of the strain with phenol as a co-substrate was further analyzed based on the cells' physiological response and the related enzymatic expression. It was found that biomass and microbial activity increased significantly, which promoted the secretion of proteins and polysaccharides in extracellular polymeric substances (EPS). And the functional groups of EPS also were changed in the co-metabolism system. In addition, compared with the non-co-metabolism system, the co-metabolism system could advance the expression of dehydrogenase (DHA) activities and dioxygenase (C23O) activities by 12 h, and the activities respectively increased by 0.17 to 3.37 times and 0.84 to 1.71, respectively, times during the degradation process. Therefore, the co-metabolism system with phenol as a co-substrate could effectively improve the degradation rate of phenanthrene, which was of great significance for the efficient degradation of aromatic hydrocarbons.
- phenol,
- phenanthrene,
- biodegradation,
- co-metabolism,
- action mechanism

FullText(HTML)

References(31)

References

[1]	WANG B C, WANG Q H, ZHU L C, et al.Degrade naphthalene using cells immobilized combining with low-intensity ultrasonic technique[J].Colloids & Surfaces B Biointerfaces, 2007,57(1):17-21.
[2]	刘兴社,刘永军,刘喆,等.煤化工废水中酚类物质、氨氮的处理方法研究进展[J].化工进展,2021,40(1):505-514.
[3]	WANG H Z, LUO J, GU L P, et al.Efficient biodegradation of phenanthrene by a novel strain Massilia sp.WF1 isolated from a PAH-contaminated soil[J].Environmental Science & Pollution Research, 2016, 23(13):378-388.
[4]	GUO J Y, WEN X Y.Performance and kinetics of benzo(a)pyrene biodegradation in contaminated water and soil and improvement of soil properties by biosurfactant amendment[J].Ecotoxicology and Environmental Safety, 2021,207:111292.
[5]	尹向阳.煤化工废水特征污染物降解菌的筛选及功能菌群降解性能研究[D].青岛:青岛科技大学,2019.
[6]	胡肖怡.焦化废水中多环芳烃生物降解的促进机制[D].广州:华南理工大学,2018.
[7]	刘羽.兰炭废水中有机污染物的去除规律及喹啉类有机物生物转化特征研究[D].西安:西安建筑科技大学,2019.
[8]	王语笑.Advenella sp.B9与Stenotrophomonas sp.N5共培养对苯酚、荧蒽的协同降解研究[D].广州:华南理工大学, 2019.
[9]	KHAN M M, TAKIZAWA S, LEWANDOWSKI Z, et al.Combined effects of EPS and HRT enhanced biofouling on a submerged and hybrid PAC-MF membrane bioreactor[J].Water Research, 2013, 47(2):747-757.
[10]	LIU Z, ZHANG X H, ZHANG S M, et al.A comparison between exogenous carriers enhanced aerobic granulation under low organic loading in the aspect of sludge characteristics, extracellular polymeric substances and microbial communities[J].Bioresource Technology,2022,346:126567-126567.
[11]	张巧, 李永成.Vibrio natriegens几丁质酶基因的异源表达及酶学性质研究[J].食品科技,2022,47(2):10-17.
[12]	杨玉霞, 李德鹏, 梁含笑, 等.紫外可见分光光度法测定植物叶中甲醛脱氢酶活性的教学实验设计[J].实验室研究与探索,2020,39(9):208-210.
[13]	WANG P, ZHANG Y M, JIN J, et al.A high-efficiency phenanthrene-degrading Diaphorobacter sp.isolated from PAH-contaminated river sediment[J].Science of the Total Environment, 2020, 746:140455.
[14]	阮珍, 刘永军, 刘静,等.金属离子对赤红球菌(Rhodococcus ruber L9)降解芘的影响及其作用机制[J].环境化学, 2019, 38(12):2649-2656.
[15]	CAI Y X, YAN Z Y, OU Y J, et al.Effects of different carbon sources on the removal of ciprofloxacin and pollutants by activated sludge:mechanism and biodegradation[J].Journal of Environmental Sciences, 2022,111:240-248.
[16]	杨廷浩,黄开,陈显峰.污水反硝化过程中外碳源乙酸钠最佳投加点研究[J].净水技术,2022,41(增刊2):87-93.
[17]	QIAN H, CHENG Y, YANG C P, et al.Performance and biofilm characteristics of biotrickling filters for ethylbenzene removal in the presence of saponins[J].Environmental Science & Pollution Research International, 2017, 25(2):30021-30030.
[18]	SHENG G P, ZHANG M L, YU H Q.Characterization of adsorption properties of extracellular polymeric substances (EPS) extracted from sludge[J].Colloids & Surfaces B Biointerfaces, 2008, 62(1):83-90.
[19]	GUO W D, JING X, WANG J P, et al.Characterization of dissolved organic matter in urban sewage using excitation emission matrix fluorescence spectroscopy and parallel factor analysis[J].Journal of Environmental Sciences, 2010, 22(11):1728-1734.
[20]	薛静静, 张伟, 郭惠娟, 等.溶藻细菌Paenibacillus sp.XXG溶藻产物光谱分析[J].环境化学,2021,40(7):2083-2091.
[21]	WANG J, LI Q, LI M M, et al.Competitive adsorption of heavy metal by extracellular polymeric substances (EPS) extracted from sulfate reducing bacteria[J].Bioresource Technology, 2014, 163:374-376.
[22]	WANG Z W, WU Z H, XING Y, et al.Membrane fouling in a submerged membrane bioreactor (MBR) under sub-critical flux operation:membrane foulant and gel layer characterization[J].Journal of Membrane Science, 2008, 325(1):238-244.
[23]	WEI X, FANG L C, CAI P, et al.Influence of extracellular polymeric substances (EPS) on Cd adsorption by bacteria[J].Environmental Pollution, 2011,159(5):1369-1374.
[24]	ZHANG Y P, WANG F, ZHU X S, et al.Extracellular polymeric substances govern the development of biofilm and mass transfer of polycyclic aromatic hydrocarbons for improved biodegradation[J].Bioresource Technology,2015,193:274-280.
[25]	XIAO M, YIN X Y, GAI H J, et al.Effect of hydroxypropyl-beta-cyclodextrin on the cometabolism of phenol and phenanthrene by a novel Chryseobacterium sp[J].Bioresource Technology, 2019, 273:56-62.
[26]	CAO B, GENG A L, LOH K C.Induction of ortho- and meta-cleavage pathways in Pseudomonas in biodegradation of high benzoate concentration:MS identification of catabolic enzymes[J].Applied Microbiology & Biotechnology, 2008, 81(1):99-107.
[27]	BALACHANDRAN C, DURAIPANDIYAN V, BALAKRISHNA K, et al.Petroleum and polycyclic aromatic hydrocarbons (PAHs) degradation and naphthalene metabolism in Streptomyces sp.(ERI-CPDA-1) isolated from oil contaminated soil[J].Bioresource Technology, 2012, 112:83-90.
[28]	RENÉ V H, BORIS J, ANNEMIE R, et al.Effect of bioaugmentation and supplementary carbon sources on degradation of polycyclic aromatic hydrocarbons by a soil-derived culture[J].Fems Microbiology Ecology, 2010, 55(1):122-135.
[29]	HAN Z X, MENG Z, LV C X.Effect factors of biodegradation on phenanthrene by pseudomonas putida[J].Advanced Materials Research, 2011, 396/397/398:2107-2110.
[30]	LEE D S, LEE M W, WOO S H, et al.Effects of Salicylate and Glucose on Biodegradation of Phenanthrene by Burkholderia cepacia PM07[J].Journal of Microbiology and Biotechnology, 2005, 15(4):859-865.
[31]	LIU N, LI D, LI K, et al.Enhanced biodegradation of chlorobenzene via combined Fe³⁺ and Zn²⁺ based on rhamnolipid solubilisation[J].Journal of Environmental Sciences, 2021, 103(1):108-118.