ISOLATION, IDENTIFICATION AND DEGRADATION CHARACTERISTICS OF STRAINS FOR REMEDIATION OF PETROLEUM HYDROCARBON UNDER ARSENIC STRESS

LI Hongcheng; SU Qu; ZHANG Wuzhu; ZHANG Yao; XIANG Luojing

doi:10.13205/j.hjgc.202307023

Volume 41 Issue 7

Jul. 2023

Turn off MathJax

Article Contents

Abstract

References

Article Navigation > ENVIRONMENTAL ENGINEERING > 2023 > 41(7): 166-174

LI Hongcheng, SU Qu, ZHANG Wuzhu, ZHANG Yao, XIANG Luojing. ISOLATION, IDENTIFICATION AND DEGRADATION CHARACTERISTICS OF STRAINS FOR REMEDIATION OF PETROLEUM HYDROCARBON UNDER ARSENIC STRESS[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(7): 166-174. doi: 10.13205/j.hjgc.202307023

Citation:

LI Hongcheng, SU Qu, ZHANG Wuzhu, ZHANG Yao, XIANG Luojing. ISOLATION, IDENTIFICATION AND DEGRADATION CHARACTERISTICS OF STRAINS FOR REMEDIATION OF PETROLEUM HYDROCARBON UNDER ARSENIC STRESS[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(7): 166-174. doi: 10.13205/j.hjgc.202307023

Citation:

LI Hongcheng, SU Qu, ZHANG Wuzhu, ZHANG Yao, XIANG Luojing. ISOLATION, IDENTIFICATION AND DEGRADATION CHARACTERISTICS OF STRAINS FOR REMEDIATION OF PETROLEUM HYDROCARBON UNDER ARSENIC STRESS[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(7): 166-174. doi: 10.13205/j.hjgc.202307023

PDF( 6285 KB)

ISOLATION, IDENTIFICATION AND DEGRADATION CHARACTERISTICS OF STRAINS FOR REMEDIATION OF PETROLEUM HYDROCARBON UNDER ARSENIC STRESS

doi: 10.13205/j.hjgc.202307023

LI Hongcheng^1,2,
SU Qu^1,2,
ZHANG Wuzhu^1,2,3,
ZHANG Yao^1,2,
XIANG Luojing^{1,2
,
,}

1. Hubei Academy of Environmental Science, Wuhan 430072, China;
2. State Key Laboratory of Soil Health Diagnosis and Green Remediation for Environmental Protection, Wuhan 430072, China;
3. College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China

Received Date: 2022-09-14

Abstract

Abstract

In this study, petroleum hydrocarbon-degrading strains were isolated and purified from an arsenic-petroleum hydrocarbon composite contaminated site in Hubei Province. 16S rDNA sequencing technology was used to identify the bacteria species. The growth and degradation characteristics of the degrading strain were analyzed, and the actual remediation ability of the degrading strains was tested on the composite contaminated soil. The main conclusions were as follows:Acinetobacter sp. JYZ-03, a highly efficient petroleum hydrocarbon degradation strain resistant to As, was isolated. The optimal growth and degradation conditions were pH of 7, 30℃, salinity of 0.1% and initial inoculation amount of 2%. The degradation rate of petroleum hydrocarbon by strain JYZ-03 was 84.05%. The degradation ability of strain for different petroleum hydrocarbon components was in sequence of long-chain alkane (C26 to C38)>polycyclic aromatic hydrocarbons>branched alkanes>medium and long-chain alkanes (C11 to C25); the actual repairing by the strain achieved a satisfying effect. This study enriches the functional strains for remediation of petroleum hydrocarbon, and provides more options for the remediation of compound-contaminated sites.
- petroleum hydrocarbon,
- arsenic,
- degradation bacteria,
- soil combined pollution,
- bio-remediation

FullText(HTML)

References(43)

References

[1]	胡迪,李川,董倩倩,等.油田区土壤石油烃组分残留特性研究[J].环境科学, 2014, 35(1):227-232.
[2]	FARRINGTON JW.Oil pollution in the marine environment ii:fates and effects of oil spills[J].Environment, 2014, 56:16-31.
[3]	LIU X Y, JI X Y, LI X W, et al.Migration of petroleum-series contaminants in the soil[J].Soils, 2005, 37:482-486.
[4]	王红旗, 刘新会, 李国学.土壤环境学[B].北京:高等教育出版社, 2007.
[5]	WEI Y, CAI M, ZHU K, et al.Effects of oil pollution on soil moisture[J].Journal of Earth Environment, 2018, 9:266-272.
[6]	OSUJI L C, ONOJAKE C M.Trace heavy metals associated with crude oil:a case study of Ebocha-8 oil-spill-polluted site in Niger Delta, Nigeria[J].Chemistry & Biodiversity, 2004, 1:1708-1715.
[7]	李彦辰.石油烃降解菌的筛选及降解特性研究[D].哈尔滨:哈尔滨工业大学, 2021.
[8]	孙娟, 王宁, 陈宏坤, 等.石油污染土壤微生物群落分布特征[J].石油学报(石油加工), 2021, 37(2):372-383.
[9]	曾秋玲.石油烃降解菌的筛选及降解特性研究[D].大连:大连海事大学, 2020.
[10]	袁林杰, 彭芃, 申泰铭, 等.石油烃降解菌的研究进展[J].生命科学, 2019, 31(10):1088-1097.
[11]	HODSON P V.The Toxicity to fish embryos of pah in crude and refined oils[J].Archives of Environmental Contamination and Toxicology, 2017, 73:12-18.
[12]	周际海,黄荣霞,樊后保,等.污染土壤修复技术研究进展[J].水土保持研究,2016,23(3):366-372.
[13]	孔令姣.石油污染土壤的生物修复及细菌多样性研究[D].兰州:兰州理工大学, 2017.
[14]	CHEN H Q, LI X C, YU X F, et al.A review on technique progresses of microbial diversity in soil ecosystem[J].Earth and Environment, 2018, 46:204-209.
[15]	AL FARRAJ D A, ALKUFEIDY R M, ALKUBAISI N A, et al.Polynuclear aromatic anthracene biodegradation by psychrophilic Sphingomonas sp.,cultivated with tween-80[J].Chemosphere, 2021, 263:128115.
[16]	CHANDRAN P, DAS N.Degradation of diesel oil by immobilized Candida tropicalis and biofilm formed on gravels[J].Biodegradation 2011,22:1181-1189.
[17]	DENG M C, LI J, LIANG F R, et al.Isolation and characterization of a novel hydrocartbon-degrading bacterium Achromobacter sp.HZ01 from the cnude oil-contaminated seawater at the Daya Bay, southemn ChinaC[J].Marine Pollution Bulletin, 2014, 83:79-86.
[18]	GUAN X H, XIE P.Literature review of combined pollution of metal ions and organic pollutants[J].Journal of Civil and Environmental Engineering, 2019, 41:120-128.
[19]	KRONBERG R M, JISKRA M, WIEDERHOLD J G, et al.Methyl mercury formation in hillslope soils of boreal forests:the role of forest harvest and anaerobic microbes[J].Environmental Science & Technology, 2018, 52:367-367.
[20]	LENCHI N, KEBBOUCHE-GANA S, SERVAIS P, et al.Diesel biodegradation capacities and biosurfactant production in saline-alkaline conditions by delftia sp nl1, isolated from an Algerian oilfield[J].Geomicrobiology Journal, 2020, 37:454-466.
[21]	SONG J W, SHEN Q L, WANG L, et al.Effects of Cd, Cu, Zn and their combined action on microbial biomass and bacterial community structure[J].Environmental Pollution, 2018, 243:510-518.
[22]	TZOVOLOU D N, TSAKIROGLOU C D.Experimental study of in situ remediation of lowpermeability soils by BIO VENTING[J].Environmental Engineering and Management Journal, 2018, 17:2645-2656.
[23]	BARSHA M J, DAS S,PAL B Y, et al.Evaluation of arsenic induced toxicity based on arsenic accumulation, translocation and its implications on physio-chemical changes and genomic instability in indica rice (Oryza sativa L.) cultivars[J].Ecotoxicology (London, England), 2020,29(1):13-34.
[24]	GEETS J, COOMAN M D, WITTEBOLLE L, et al.Real-time PCR assay for the simultaneous quantification of nitrifying and denitrifying bacteria in activated sludge[J].Applied Microbiology & Biotechnology, 2007, 75(1):211-221.
[25]	生态环境部.水质可萃取性石油烃(C10-C40)的测定气相色谱法:HJ 894-2017[S].北京:生态环境部,2018.
[26]	生态环境部.土壤和沉积物石油烃(C10-C40)的测定气相色谱法:HJ 1021-2019[S].北京:生态环境部,2018.
[27]	PALANISAMY N, RAMYA J, KUMAR S, et al.Diesel biodegradation capacities of indigenous bacterial species isolated from diesel contaminated soil[J].Journal of Environmental Health Science & Engineering, 2014,12(1):142.
[28]	WHANG L M, LIU P W G, MA C C, et al.Application of rhamnolipid and surfactin for enhanced diesel biodegradation-Effects of pH andammonium addition[J].J Hazard Mater, 2009, 164:1045-1050.
[29]	XIA W, LI J, XIA Y, et al.Optimization of diesel oilbiodegradation in seawater using statistical experimental methodology[J].Water Sci Technol, 2012, 66:1301-1309.
[30]	GREENWOOD P F, WIBROW S, GEORGE S J, et al.Sequential hydro-carbonbiodegradation in a soil from arid coastal Australia treated with oil underlaboratory controlled conditions[J].Org Geochem, 2008, 39:1336-1346.
[31]	LUO Q, ZHANG J G, SHEN X R, et al.Characterization of a noveldiesel oil-degrading pseudomonas sp.strain F4[J].Fresenius Environ Bullet, 2013, 22:689-697.
[32]	MNIF I, SAHNOUN R, ELLOUZE-CHAABOUNI S, et al.Evaluation of B.subtilisSPB1 biosurfactants' potency for diesel-contaminated soil washing:optimization of oil desorption using Taguchi design[J].Environ Sci Pollut Res, 2014, 21:851-861.
[33]	LIU M H, TSAI C F, CHEN B Y.Biodegradation kinetics of diesel in a wind-driven bioventing system[J].The Journal of Korean Society of Soil and Groundwater Environment, 2016, 21:8-15.
[34]	杨茜.石油烃降解菌的筛选及石油污染土壤的生物修复机理研究[D].西安:西安建筑科技大学, 2014.
[35]	WANG Q S, HE M C, WANG Y.Influence of combined pollution of antimony and arsenic on culturable soil microbial populations and enzyme activities[J].Ecotoxicology, 2011, 20:9-19.
[36]	KAUR D, SINGH R P, GUPTA S.Screening and characterization of next-generation biofuels producing bacterial strains[J].Current Microbiology, 2022, 79.
[37]	胡梦杰,钟磊,蔡晓鲜,等.微生物降解石油烃的代谢机制及研究进展[J].环境工程,2023,41(2):234-246.
[38]	SOWANI H, KULKARNI M, ZINJARDE S.Uptake and detoxification of diesel oil by a tropical soil Actinomycete Gordonia amicalis HS-11:Cellular responses and degradation perspectives[J].Environmental Pollution, 2020, 263:114538.
[39]	DAS N, CHANDRAN P.Microbial degradation of petroleum hydrocarbon contaminants:an overview[J].Biotechnol Res Int, 2011, 2011:941810.
[40]	JI Y, MAO G, WANG Y, et al.Structural insights into diversity and n-alkane biodegradation mechanisms of alkane hydroxylases[J].Frontiers in Microbiology, 2013, 4:58.
[41]	ABBASIAN F, LOCKINGTON R, MALLAVARAPU M, et al.A Comprehensive Review of Aliphatic Hydrocarbon Biodegradation by Bacteria[J].Applied Biochemistry and Biotechnology, 2015, 176(3):670-699.
[42]	CABRAL L, GIOVANELLA P, PELLIZZER E P, et al.Microbial communities in petroleum-contaminated sites:structure and metabolisms[J].Chemosphere, 2022, 286:131752.
[43]	PALANISAMY N,RAMYA J,KUMAR S, et al.Diesel biodegradation capacities of indigenous bacterial species isolated from diesel contaminated soil[J].Journal of Environmental Health Science & Engineering, 2014, 12:142.

Relative Articles

[1]	LI Yuping, FAN Baoyun, DONG Kangran, WAN Jinzhong, AI Yingbo, WANG Baotian. EXPERIMENTAL STUDY ON THERMAL REMEDIATION OF PETROLEUM HYDROCARBON CONTAMINATED SOILS[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(4): 242-249. doi: 10.13205/j.hjgc.202404028
[2]	DING Ning, ZUO Shiwei, ZHANG Ruibo, WANG Zhaohui, LI Kewen, SHANG Ershun. SOLUBILIZER DESIGN FOR INTENSIFYING REMEDIATION OF SOIL WITH PETROLEUM HYDROCARBON POLLUTANTS[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(1): 177-183. doi: 10.13205/j.hjgc.202401023
[3]	CAO Lixia, LI Wenshuan, LIN Xin, LI Xiaojun, FU Wanlong. EFFECTS OF SELENIUM APPLICATION ON ARSENIC UPTAKE AND ACCUMULATION IN RICE[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(7): 271-276. doi: 10.13205/j.hjgc.202307036
[4]	LI Shefeng, DU Shaoxia, BAO Shenxu, YAN Shuiping, LIU Ziyang. BIBLIOMETRIC ANALYSIS AND DEVELOPMENT TREND DISCUSSION OF CONTAMINATED SOIL REMEDIATION TECHNOLOGY IN INTERNATIONAL RESEARCH[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(12): 329-336,342. doi: 10.13205/j.hjgc.202312041
[5]	HU Mengjie, ZHONG Lei, CAI Xiaoxian, QING Jinwu, SUN Yuru, LI Gaoyuan, RUAN Haihua, CHEN Guanyi. METABOLIC MECHANISM OF MICROBIAL DEGRADATION OF PETROLEUM HYDROCARBONS AND ITS RESEARCH PROGRESS[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(2): 234-246. doi: 10.13205/j.hjgc.202302031
[6]	XU Jinlan, YANG Zhengli. IMPACTS OF PETROLEUM HYDROCARBONS BIODEGRADATION IN OIL-CONTAMINATED SOIL AFTER PRE-OXIDATION WITH THREE BATCHS H₂O₂ ADDITION[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(2): 122-130. doi: 10.13205/j.hjgc.202302017
[7]	YAN Wenming, JIANG Chao, CHEN Xiang, MA Lin, YAN Binglong, HE Xiangyu, LI Minjuan, TIAN Fen, WU Tingfeng. EFFECT OF TWO COVERING AGENTS ON PASSIVATION OF SIMULATED ARSENIC CONTAMINATED SEDIMENTS BY MICROSCALE TECHNIQUES[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(11): 127-133,151. doi: 10.13205/j.hjgc.202211018
[8]	LI Yalin, LI Peng, TANG Yifan, ZHANG Wei, WANG Enci, JIN Mingyu. IMPACT OF DC VOLTAGE ON ELECTRO-REMEDIATION OF Pb AND As CONTAMINATED SOIL[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(8): 131-135,184. doi: 10.13205/j.hjgc.202208018
[9]	SHI Wenwen, WEI Xing, ZHOU Jinlong, LEI Mi, ZENG Yanyan. ADSORPTION OF PETROLEUM POLLUTANTS ON DIFFERENT TEXTURE SOILS IN XINJIANG[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(4): 127-133. doi: 10.13205/j.hjgc.202204018
[10]	ZHANG Li, GUO Chao-hui, RAN Hong-zhen, XIAO Xi-yuan, HU Zhi-hao, LI Zhang-zhou. PARTICLE SIZE AND OCCURRENCE CHARACTERISTICS OF ARSENIC IN RIVER SEDIMENTS OF ARSENIC-BEARING MINE AREAS[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(12): 38-43,119. doi: 10.13205/j.hjgc.202112006
[11]	FANG Qing, XIAN Ping, MENG Zheng-cheng. ENVIRONMENTAL HEALTH RISK ASSESSMENT MODEL OF AGRICULTURAL LAND BASED ON MONTE CARLO SIMULATION AND ITS APPLICATION[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(2): 147-152. doi: 10.13205/j.hjgc.202102024
[12]	WANG Hua-wei, WU Ya-jing, XU Rong, SUN Ying-jie, LI Shu-peng, WANG Ya-nan, ZHONG Chen-yu, SHI Chang-fei. STABILIZATION OF ARSENIC IN CONTAMINATED SOILS USING BIOLOGICAL Mn OXIDE (Bio-MnO_x)[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(9): 205-210,216. doi: 10.13205/j.hjgc.202109029
[13]	QIU Ya-qun, LI Yi-hua, PENG Pei-qin, LI Er-ping, YU Zhen-hua. EFFECT OF CHELATING AGENT ON PTERIS VITTATA FOR REMEDIATION OF ARSENIC-CONTAMINATED SOIL[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(3): 204-209,119. doi: 10.13205/j.hjgc.202103029
[14]	JIA Ling-hui, ZHENG Shi-hao, SUN Li-hui, BAO Yong-ming. SCREENING OF COLD-ADAPTED PETROLEUM DEGRADING BACTERIA AND CHARACTERIZING MICROFLORA FOR PETROLEUM DEGRADATION[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(6): 252-258. doi: 10.13205/j.hjgc.202006041
[15]	CHEN Mei-feng, LI Xin-li, YANG Pei-lin, LUO Qian, QIN Fan-xin. STABILIZATION OF AS CONTAMINATED SOILS BY MODIFIED NANO-TITANIUM DIOXIDE[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(10): 222-227. doi: 10.13205/j.hjgc.202010035
[16]	ZHANG Ruo-shi, TIAN Yong-qiang. RESEARCH PROGRESS OF BIOSORPTION REMEDIATION TECHNOLOGIES FOR CHROMIUM CONTAMINATED SITES[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(11): 187-195. doi: 10.13205/j.hjgc.202011031
[17]	CHEN Jun-hua, ZHU Hong, SHAN Hui-feng, XING Yi-lan. PERFORMANCE OF SURFACTANTS ENHANCED AEROBIC BIOREMEDIATION OF PAHs CONTAMINATED SOIL[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(5): 185-190. doi: 10.13205/j.hjgc.202005032
[18]	JIA Wei, CHEN Jin-quan, CHANG Jun-jun. BIOREMEDIATION OF MERCURY CONTAMINATION: A REVIEW[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(5): 171-178. doi: 10.13205/j.hjgc.202005030
[19]	ZHOU Li-wei, WANG Hang, LIU Yang-sheng. EFFECT OF ELECTRODE-ORIENTATED ELECTROKINETIC ENHANCEMENT ON PHYTOREMEDIATION ON ARSENIC CONTAMINATED SOIL[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(10): 228-233. doi: 10.13205/j.hjgc.202010036
[20]	ZHENG Jin, WANG Xin-yu, LI Jie, SONG Quan-wei, LI Hong-li, WANG Xiao-ling, TIAN Pei-ting. BIOREMEDIATION OF CRUDE OIL IN CONTAMINATED SOIL BY MICROORGANISMS IMMOBILIZED WITH HUMIC ACID-MODIFIED BIOFUEL ASH[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(8): 34-40. doi: 10.13205/j.hjgc.202008006

Supplements(0)

Cited By

Cited by
Periodical cited type(1)
1. 赵欣鑫，刘普，高捷，李鹏飞，齐文博，翟翊博，刘智峰. 基于文献计量的细菌-生物炭协同修复重金属污染土壤研究进展. 生态学报. 2024(13): 5882-5892 .
Other cited types(1)