NITROGEN REMOVAL PERFORMANCE BY ALGAL-DRIVEN AEROBIC METHANE OXIDATION COUPLED WITH DENITRIFICATION IN A PHOTO-BIOFILM REACTOR

FU Jiachen; WANG Jing; ZHAO Yiying; WEN Huiyan; AN Xiao; CHEN Yucheng; ZHOU Zhongbo

doi:10.13205/j.hjgc.202308003

Volume 41 Issue 8

Aug. 2023

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2023 > 41(8): 18-25

FU Jiachen, WANG Jing, ZHAO Yiying, WEN Huiyan, AN Xiao, CHEN Yucheng, ZHOU Zhongbo. NITROGEN REMOVAL PERFORMANCE BY ALGAL-DRIVEN AEROBIC METHANE OXIDATION COUPLED WITH DENITRIFICATION IN A PHOTO-BIOFILM REACTOR[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(8): 18-25. doi: 10.13205/j.hjgc.202308003

Citation:

FU Jiachen, WANG Jing, ZHAO Yiying, WEN Huiyan, AN Xiao, CHEN Yucheng, ZHOU Zhongbo. NITROGEN REMOVAL PERFORMANCE BY ALGAL-DRIVEN AEROBIC METHANE OXIDATION COUPLED WITH DENITRIFICATION IN A PHOTO-BIOFILM REACTOR[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(8): 18-25. doi: 10.13205/j.hjgc.202308003

Citation:

FU Jiachen, WANG Jing, ZHAO Yiying, WEN Huiyan, AN Xiao, CHEN Yucheng, ZHOU Zhongbo. NITROGEN REMOVAL PERFORMANCE BY ALGAL-DRIVEN AEROBIC METHANE OXIDATION COUPLED WITH DENITRIFICATION IN A PHOTO-BIOFILM REACTOR[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(8): 18-25. doi: 10.13205/j.hjgc.202308003

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NITROGEN REMOVAL PERFORMANCE BY ALGAL-DRIVEN AEROBIC METHANE OXIDATION COUPLED WITH DENITRIFICATION IN A PHOTO-BIOFILM REACTOR

doi: 10.13205/j.hjgc.202308003

1. College of Resources and Environment, Southwest University, Chongqing 400715, China;
2. Chang Jiang Ecology (Hubei)Technology Development Co., Ltd., Wuhan 430015, China;
3. Chongqing Engineering Research Center of Rural Cleaner Production, Chongqing 400715, China

Received Date: 2022-07-17
Available Online: 2023-11-15

Abstract

Abstract

To reduce the high cost of external carbon sources addition, and remove the remaining nitrate of the secondary effluent in traditional wastewater treatment, an algae-bacterial photo-biofilm reactor without mechanical oxygen supply was established, by using microalgae to create a micro-oxygen environment, which achieved aerobic methane oxidation coupled with denitrification (AME-D), and finally realized nitrogen removal from wastewater. Here, the long-term performance of the sequencing batch photo-biofilm reactor was investigated, and the related microbial activity and community composition were analyzed. The algae-bacterial photo-biofilm reactor could be operated stably for 50 days, with a stable NO₃^--N removal rate of 25 mg/(L·d), and the nitrogen removal efficiency could reach 95%, which did not cause the pollution of other nitrogen elements. Activity tests showed that the denitrification rate of nitrite was higher than that of nitrate, while the enrichment or accumulation of internal carbon sources could enhance the denitrification process. High-throughput sequencing analysis confirmed the presence of algae [Chlorella (32.45%)] and Cyanobacteria [Pantanalinema_CENA516 (3.95%)], methane-oxidizing bacteria [Methylocaldum (1.39%)] and denitrifying bacteria [Thermomonas (20.32%), Stappia (7.24%), Hyphomicrobium (2.34%)], and the corresponding functional genes were further revealed by metagenomic data analysis. Overall, the photo-biofilm reactor using biogas as the carbon source achieved nitrogen removal from the wastewater without mechanical oxygen supply, which provides a new way for low-carbon and high-efficient treatment of wastewater.
- aerobic methane oxidation,
- denitrification,
- algae-bacterial consortia,
- photobioreactor,
- nitrogen removal

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References(32)

References

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