NITROGEN REMOVAL PERFORMANCE BY ALGAL-DRIVEN AEROBIC METHANE OXIDATION COUPLED WITH DENITRIFICATION IN A PHOTO-BIOFILM REACTOR

FU Jiachen; WANG Jing; ZHAO Yiying; WEN Huiyan; AN Xiao; CHEN Yucheng; ZHOU Zhongbo

doi:10.13205/j.hjgc.202308003

Volume 41 Issue 8

Aug. 2023

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FU Jiachen, WANG Jing, ZHAO Yiying, WEN Huiyan, AN Xiao, CHEN Yucheng, ZHOU Zhongbo. NITROGEN REMOVAL PERFORMANCE BY ALGAL-DRIVEN AEROBIC METHANE OXIDATION COUPLED WITH DENITRIFICATION IN A PHOTO-BIOFILM REACTOR[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(8): 18-25. doi: 10.13205/j.hjgc.202308003

Citation:

FU Jiachen, WANG Jing, ZHAO Yiying, WEN Huiyan, AN Xiao, CHEN Yucheng, ZHOU Zhongbo. NITROGEN REMOVAL PERFORMANCE BY ALGAL-DRIVEN AEROBIC METHANE OXIDATION COUPLED WITH DENITRIFICATION IN A PHOTO-BIOFILM REACTOR[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(8): 18-25. doi: 10.13205/j.hjgc.202308003

Citation:

FU Jiachen, WANG Jing, ZHAO Yiying, WEN Huiyan, AN Xiao, CHEN Yucheng, ZHOU Zhongbo. NITROGEN REMOVAL PERFORMANCE BY ALGAL-DRIVEN AEROBIC METHANE OXIDATION COUPLED WITH DENITRIFICATION IN A PHOTO-BIOFILM REACTOR[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(8): 18-25. doi: 10.13205/j.hjgc.202308003

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NITROGEN REMOVAL PERFORMANCE BY ALGAL-DRIVEN AEROBIC METHANE OXIDATION COUPLED WITH DENITRIFICATION IN A PHOTO-BIOFILM REACTOR

doi: 10.13205/j.hjgc.202308003

1. College of Resources and Environment, Southwest University, Chongqing 400715, China;
2. Chang Jiang Ecology (Hubei)Technology Development Co., Ltd., Wuhan 430015, China;
3. Chongqing Engineering Research Center of Rural Cleaner Production, Chongqing 400715, China

Received Date: 2022-07-17
Available Online: 2023-11-15

Abstract

Abstract

To reduce the high cost of external carbon sources addition, and remove the remaining nitrate of the secondary effluent in traditional wastewater treatment, an algae-bacterial photo-biofilm reactor without mechanical oxygen supply was established, by using microalgae to create a micro-oxygen environment, which achieved aerobic methane oxidation coupled with denitrification (AME-D), and finally realized nitrogen removal from wastewater. Here, the long-term performance of the sequencing batch photo-biofilm reactor was investigated, and the related microbial activity and community composition were analyzed. The algae-bacterial photo-biofilm reactor could be operated stably for 50 days, with a stable NO₃^--N removal rate of 25 mg/(L·d), and the nitrogen removal efficiency could reach 95%, which did not cause the pollution of other nitrogen elements. Activity tests showed that the denitrification rate of nitrite was higher than that of nitrate, while the enrichment or accumulation of internal carbon sources could enhance the denitrification process. High-throughput sequencing analysis confirmed the presence of algae [Chlorella (32.45%)] and Cyanobacteria [Pantanalinema_CENA516 (3.95%)], methane-oxidizing bacteria [Methylocaldum (1.39%)] and denitrifying bacteria [Thermomonas (20.32%), Stappia (7.24%), Hyphomicrobium (2.34%)], and the corresponding functional genes were further revealed by metagenomic data analysis. Overall, the photo-biofilm reactor using biogas as the carbon source achieved nitrogen removal from the wastewater without mechanical oxygen supply, which provides a new way for low-carbon and high-efficient treatment of wastewater.
- aerobic methane oxidation,
- denitrification,
- algae-bacterial consortia,
- photobioreactor,
- nitrogen removal

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References(32)

References

[1]	王熹,王湛,杨文涛,等.中国水资源现状及其未来发展方向展望[J].环境工程,2014,32(7):1-5.
[2]	崔琦,陈卓,李魁晓,等.再生水系统的可靠性:内涵及其保障措施[J].环境工程,2019,37(12):75-79 ,108.
[3]	WANG H J,LI Y Y,ZHANG S Q,et al.Effect of influent feeding pattern on municipal tailwater treatment during a sulfur-based denitrification constructed wetland[J].Bioresource Technology,2020,315:123807.
[4]	FU X R,HOU R R,YANG P,et al.Application of external carbon source in heterotrophic denitrification of domestic sewage:a review[J].The Science of the Total Environment,2022,817:153061.
[5]	王开乐,周集体,田天,等.剩余污泥碱解液用作低C/N合成氨废水反硝化碳源研究[J].环境工程,2021,39(12):31-37.
[6]	CHEN H,LIU S,LIU T,et al.Efficient nitrate removal from synthetic groundwater via in situ utilization of short-chain fatty acids from methane bioconversion[J].Chemical Engineering Journal,2020,393:124594.
[7]	ZHU J,WANG Q,YUAN M D,et al.Microbiology and potential applications of aerobic methane oxidation coupled to denitrification (AME-D) process:a review[J].Water Research,2016,90:203-215.
[8]	严程,梅娟,赵由才.好氧甲烷氧化菌及其工程应用进展[J].生物工程学报,2022,38(4):1322-1338.
[9]	COSTA R B,LENS P N L,FORESTI E.Methanotrophic denitrification in wastewater treatment:microbial aspects and engineering strategies[J].Critical Reviews in Biotechnology,2022,42(1):145-161.
[10]	ZHANG B,LI W,GUO Y,et al.Microalgal-bacterial consortia:from interspecies interactions to biotechnological applications[J].Renewable and Sustainable Energy Reviews,2020,118:109563.
[11]	刁梦洁,柳杰,王晚晴,等.菌藻共生对污水处理和微藻生物量积累的影响[J].环境工程,2018,36(3):8-12.
[12]	RAMANAN R,KIM B H,CHO D H,et al.Algae-bacteria interactions:evolution,ecology and emerging applications[J].Biotechnology Advances,2016,34(1):14-29.
[13]	MANSER N D,WANG M,ERGAS S J,et al.Biological nitrogen removal in a photosequencing batch reactor with an algal-nitrifying bacterial consortium and anammox granules[J].Environmental Science & Technology Letters,2016,3(4):175-179.
[14]	BOELEE N C,TEMMINK H,JANSSEN M,et al.Nitrogen and phosphorus removal from municipal wastewater effluent using microalgal biofilms[J].Water Research,2011,45(18):5925-5933.
[15]	国家环境保护总局,《水和废水监测分析方法》编委会.水和废水监测分析方法[M].4版.北京:中国环境科学出版社,2002.
[16]	CALLAHAN B J,MCMURDIE P J,ROSEN M J,et al.DADA2:high-resolution sample inference from Illumina amplicon data[J].Nature Methods,2016,13(7):581-583.
[17]	MCILROY S J,SAUNDERS A M,ALBERTSEN M,et al.MiDAS:the field guide to the microbes of activated sludge[J].Database-the Journal of Biological Databases and Curation,2015:1-8.
[18]	QIAN L,YU X L,ZHOU J Y,et al.MCycDB:a curated database for comprehensively profiling methane cycling processes of environmental microbiomes[J].Molecular Ecology Resources,2022,22(5):1803-1823.
[19]	SUN F Y,DONG W Y,SHAO M F,et al.Aerobic methane oxidation coupled to denitrification in a membrane biofilm reactor:treatment performance and the effect of oxygen ventilation[J].Bioresource Technology,2013,145:2-9.
[20]	周祥玉,赵云飞,李东,等.微氧条件下以甲烷为碳源的反硝化实验研究[J].环境科学学报,2017,37(5):1704-1710.
[21]	VINCENT R,M L A,JOHNNY G,et al.Nitrite accumulation during denitrification depends on the carbon quality and quantity in wastewater treatment with biofilters[J].Environmental Science and Pollution Research International,2015,22(13):10179-10188.
[22]	KHIN T,ANNACHHATRE A P.Nitrogen removal in a fluidized bed bioreactor by using mixed culture under oxygen-limited conditions[J].Water Science and Technology,2004,50(6):313-320.
[23]	LI W W,LU P L,CHAI F G,et al.Long-term nitrate removal through methane-dependent denitrification microorganisms in sequencing batch reactors fed with only nitrate and methane[J].Amb Express,2018,8(1):108.
[24]	SHASHIREKHA V,KRISHNA P P,SESHADRI S.Integrated approach for carbon sequestration and wastewater treatment using algal-bacterial Consortia:opportunities and challenges[J].Sustainability,2022,14(3):1075.
[25]	周圆,支丽玲,郑凯凯,等.城镇污水处理厂活性污泥反硝化速率的影响因素及优化运行研究[J].环境工程,2020,38(7):100-108.
[26]	张衡,王婧,王斌,等.不同环境因素对厌氧甲烷氧化型自养反硝化系统脱氮性能的影响及其微生物群落分析[J].环境工程学报,2021,15(11):3707-3717.
[27]	TIAN T,ZHOU K,XUAN L,et al.Exclusive microbially driven autotrophic iron-dependent denitrification in a reactor inoculated with activated sludge[J].Water Research,2020,170(C):115300.
[28]	ARAUJO G S,SILVA J W A,VIANA C A S,et al.Effect of sodium nitrate concentration on biomass and oil production of four microalgae species[J].International Journal of Sustainable Energy,2020,39(1):41-50.
[29]	高静思,朱佳,董文艺.光照对我国常见藻类的影响机制及其应用[J].环境工程,2019,37(5):111-116.
[30]	JIN L,JIN C Z,LEE H G,et al.Genomic insights into denitrifying methane-oxidizing bacteria Gemmobacter fulva sp.Nov.,isolated from an anabaena culture[J].Microorganisms,2021,9(12):2423.
[31]	WANG S,LIU Q X,LI J,et al.Methane in wastewater treatment plants:status,characteristics,and bioconversion feasibility by methane oxidizing bacteria for high value-added chemicals production and wastewater treatment[J].Water Research,2021,198(2):117122.
[32]	YANG R,YUAN L,WANG R.Enzymatic regulation of N₂O production by denitrifying bacteria in the sludge of biological nitrogen removal process[J].Science of the Total Environment,2022,846:157513.