MICROBIAL COMMUNITY SUCCESSION DURING LIQUID MANURE FERMENTATION AND ITS CORRELATION WITH ENVIRONMENTAL FACTORS

ZHONG Yiwen; SU Wenxing; JIANG Shan; WANG Yinhong; LIU Wangrong; WU Genyi; ZENG Dong; CHEN Lei

doi:10.13205/j.hjgc.202308018

Volume 41 Issue 8

Aug. 2023

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2023 > 41(8): 145-153

JIE Ya-wei, XU Ran-yun, DING Wei, JIANG Yi-heng, ZHANG Ben, LIU Hong-yuan. AOX FORMATION DURING THE ADVANCED OXIDATION OF PHENOL WASTEWATER CONTAINING CHLORIDE ION[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(5): 1-8. doi: 10.13205/j.hjgc.202205001

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ZHONG Yiwen, SU Wenxing, JIANG Shan, WANG Yinhong, LIU Wangrong, WU Genyi, ZENG Dong, CHEN Lei. MICROBIAL COMMUNITY SUCCESSION DURING LIQUID MANURE FERMENTATION AND ITS CORRELATION WITH ENVIRONMENTAL FACTORS[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(8): 145-153. doi: 10.13205/j.hjgc.202308018

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MICROBIAL COMMUNITY SUCCESSION DURING LIQUID MANURE FERMENTATION AND ITS CORRELATION WITH ENVIRONMENTAL FACTORS

doi: 10.13205/j.hjgc.202308018

1. South China Institute of Environmental Sciences, MEE, Guangzhou 510530, China;
2. Inner Mongolia Youran Dairy Co., Ltd., Hohhot 010000, China;
3. College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China

Received Date: 2022-10-13
Available Online: 2023-11-15

Abstract

Abstract

To study the changes in physical and chemical indices and the succession characteristics of the microbial community structure in the process of manure fermentation, using the aerobic fermentation method, commercial microbial compounds including effective microorganisms(EM) and organic fermentation bacteria(OFB), were added for fermentation after dilution of dairy manure. Changes in COD, nitrogen and phosphorus during the fermentation process were analysed. The seed germination index was used as a maturity index to investigate the maturity of the liquid manure, and the structural characteristics of the bacterial community in the fermentation process were studied. The results showed that the addition of microbial compounds can effectively break down organic matter, and the liquid manure can reach the maturity stage more quickly after diluting to low (L) concentrations. The seed germination index of the L-OFB treatment reached 84.01% at the end of fermentation, indicating full maturity. Alpha diversity and species difference analysis using high-throughput sequencing showed that the main dominant phyla in the fermentation process were Proteobacteria, Bacteroidetes and Firmicutes. During the fermentation period, the relative abundance of the ML635J-40 aquatic group, Truepera, Aquamicrobium, Anaerovorax and Pseudomonas was higher in the samples treated with EM and OFB than in the control. The results of the redundancy analysis showed that total nitrogen was the prime factor significantly correlated with the change in bacterial community composition (P<0.05). The correlation between seed germination index and Aquamicrobium was the strongest, and the relative abundance was high at the end of fermentation, which could be used as an indicator microorganism of maturity. This study can provide a theoretical basis for future optimisation of the liquid manure treatment process.
- liquid manure,
- fermentation,
- microbial agents,
- bacterial community,
- environmental factors

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