MICROBIAL COMMUNITY SUCCESSION DURING LIQUID MANURE FERMENTATION AND ITS CORRELATION WITH ENVIRONMENTAL FACTORS

ZHONG Yiwen; SU Wenxing; JIANG Shan; WANG Yinhong; LIU Wangrong; WU Genyi; ZENG Dong; CHEN Lei

doi:10.13205/j.hjgc.202308018

Volume 41 Issue 8

Aug. 2023

Turn off MathJax

Article Contents

Article Navigation > ENVIRONMENTAL ENGINEERING > 2023 > 41(8): 145-153

ZHONG Yiwen, SU Wenxing, JIANG Shan, WANG Yinhong, LIU Wangrong, WU Genyi, ZENG Dong, CHEN Lei. MICROBIAL COMMUNITY SUCCESSION DURING LIQUID MANURE FERMENTATION AND ITS CORRELATION WITH ENVIRONMENTAL FACTORS[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(8): 145-153. doi: 10.13205/j.hjgc.202308018

Citation:

ZHONG Yiwen, SU Wenxing, JIANG Shan, WANG Yinhong, LIU Wangrong, WU Genyi, ZENG Dong, CHEN Lei. MICROBIAL COMMUNITY SUCCESSION DURING LIQUID MANURE FERMENTATION AND ITS CORRELATION WITH ENVIRONMENTAL FACTORS[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(8): 145-153. doi: 10.13205/j.hjgc.202308018

Citation:

ZHONG Yiwen, SU Wenxing, JIANG Shan, WANG Yinhong, LIU Wangrong, WU Genyi, ZENG Dong, CHEN Lei. MICROBIAL COMMUNITY SUCCESSION DURING LIQUID MANURE FERMENTATION AND ITS CORRELATION WITH ENVIRONMENTAL FACTORS[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(8): 145-153. doi: 10.13205/j.hjgc.202308018

PDF( 5244 KB)

MICROBIAL COMMUNITY SUCCESSION DURING LIQUID MANURE FERMENTATION AND ITS CORRELATION WITH ENVIRONMENTAL FACTORS

doi: 10.13205/j.hjgc.202308018

1. South China Institute of Environmental Sciences, MEE, Guangzhou 510530, China;
2. Inner Mongolia Youran Dairy Co., Ltd., Hohhot 010000, China;
3. College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China

Received Date: 2022-10-13
Available Online: 2023-11-15

Abstract

Abstract

To study the changes in physical and chemical indices and the succession characteristics of the microbial community structure in the process of manure fermentation, using the aerobic fermentation method, commercial microbial compounds including effective microorganisms(EM) and organic fermentation bacteria(OFB), were added for fermentation after dilution of dairy manure. Changes in COD, nitrogen and phosphorus during the fermentation process were analysed. The seed germination index was used as a maturity index to investigate the maturity of the liquid manure, and the structural characteristics of the bacterial community in the fermentation process were studied. The results showed that the addition of microbial compounds can effectively break down organic matter, and the liquid manure can reach the maturity stage more quickly after diluting to low (L) concentrations. The seed germination index of the L-OFB treatment reached 84.01% at the end of fermentation, indicating full maturity. Alpha diversity and species difference analysis using high-throughput sequencing showed that the main dominant phyla in the fermentation process were Proteobacteria, Bacteroidetes and Firmicutes. During the fermentation period, the relative abundance of the ML635J-40 aquatic group, Truepera, Aquamicrobium, Anaerovorax and Pseudomonas was higher in the samples treated with EM and OFB than in the control. The results of the redundancy analysis showed that total nitrogen was the prime factor significantly correlated with the change in bacterial community composition (P<0.05). The correlation between seed germination index and Aquamicrobium was the strongest, and the relative abundance was high at the end of fermentation, which could be used as an indicator microorganism of maturity. This study can provide a theoretical basis for future optimisation of the liquid manure treatment process.
- liquid manure,
- fermentation,
- microbial agents,
- bacterial community,
- environmental factors

FullText(HTML)

References(41)

References

[1]	康健.畜禽粪便堆肥过程中物质转化和微生物种群演变规律及酶活性机理研究[D].兰州:兰州理工大学,2019.
[2]	李莉,杨昕涧,何家俊,等.我国畜禽粪便资源化利用的现状及展望[J].中国奶牛,2020,367(11):55-60.
[3]	孟祥海.中国畜牧业环境污染防治问题研究[D].武汉:华中农业大学,2014.
[4]	岳丹,王磊,乔莉娟,等.高效纤维素降解菌株筛选及其复合微生物菌剂在有机堆肥中的应用效果[J].江苏农业科学,2018,46(17):273-276.
[5]	孙旭,郝玉敏,苏良湖,等.微生物菌剂对稻秆-猪粪-蘑菇渣堆肥腐熟进程及品质的影响[J].安徽农业科学,2016,44(27):167-171.
[6]	席北斗,刘鸿亮,孟伟,等.垃圾堆肥高效复合微生物菌剂的制备[J].环境科学研究,2003,16(2):58-60 ,64.
[7]	王信,蔡晓剑,王亚艺,等.牛粪高温好氧堆肥中发酵菌剂筛选研究[J].青海大学学报,2019,37(5):20-25.
[8]	张晓波.紫色硫细菌Marichromatium gracile YL28对海水养殖水体氮污染的生物修复[D].厦门:华侨大学,2019.
[9]	樊婷婷,时雨,王大春,等.复合菌剂生物处理生态型污水的实验研究[J].水处理技术,2016,42(8):32-35.
[10]	李欣儒.发酵液体牛粪与化肥配施对玉米生长与产量的农学效应研究[D].哈尔滨:东北农业大学,2021.
[11]	盛婧,孙国峰,郑建初.典型粪污处理模式下规模养猪场农牧结合规模配置研究Ⅰ.固液分离-液体厌氧发酵模式[J].中国生态农业学报,2015,23(2):199-206.
[12]	李耀宇.猪场粪污厌氧发酵过程解析及沼液处理工艺研究[D].湘潭:湘潭大学,2019.
[13]	QUINCE C,WALKER A W,SIMPSON J T,et al.Shotgun metagenomics,from sampling to analysis[J].Nature Biotechnology,2017,35(9):833-844.
[14]	本刊编辑部.哈希发布20 min消解COD预制管试剂[J].中国给水排水,2013,29(4):99.
[15]	袁梦冬.规模化猪场废水处理系统中氧化塘产甲烷和脱氮微生物学机理研究[D].杭州:浙江大学,2016.
[16]	卢洋洋.不同菌种组合对牛粪好氧堆肥发酵的影响研究[D].呼和浩特:内蒙古农业大学,2019.
[17]	张丽萍,刘红江,盛婧,等.发酵周期、贮存时间和过滤对沼液养分和理化性状变化的影响[J].农业资源与环境学报,2018,35(1):32-39.
[18]	武一奇.猪粪堆肥过程氮素转化与抗性基因转移机制及调控技术[D].哈尔滨:哈尔滨工业大学,2021.
[19]	李杰.不同微生物菌剂对牛粪和玉米秸秆高温腐熟的影响[D].兰州:甘肃农业大学,2013.
[20]	单德鑫.牛粪发酵过程中碳、氮、磷转化研究[D].哈尔滨:东北农业大学,2006.
[21]	汤江武,朱利中.不同堆肥条件对种子发芽指数影响的研究[J].浙江农业科学,2008,296(5):583-586.
[22]	卢洋洋,杨硕,张玉,等.不同复合微生物菌剂对牛粪堆肥效果的影响[J].家畜生态学报,2021,42(2):43-49.
[23]	张雪辰,邓双,王旭东.快腐剂对畜禽粪便堆肥过程中腐熟度的影响[J].环境工程学报,2015,9(2):888-894.
[24]	WALTERS K E,MARTINY J B H.Alpha-,beta-,and gamma-diversity of bacteria varies across habitats[J].PLoS One,2020,15(9):e0233872.
[25]	HILL T C,WALSH K A,HARRIS J A,et al.Using ecological diversity measures with bacterial communities[J].FEMS Microbiology Ecology,2003,43(1):1-11.
[26]	史龙翔,谷洁,潘洪加,等.复合菌剂提高果树枝条堆肥过程中酶活性[J].农业工程学报,2015,31(5):244-251.
[27]	王秀红,史向远,张纪涛,等.鸡粪好氧堆肥腐熟度、重金属残留及微生物菌群分析[J].山西农业科学,2021,49(9):1094-1099.
[28]	HILL V R,KAHLER A M,JOTHIKUMAR N,et al.Multistate evaluation of an ultrafiltration-based procedure for simultaneous recovery of enteric microbes in 100-liter tap water samples[J].Apply and Environmental Microbiology,2007,73(13):4218-4225.
[29]	ZHONG X Z,LI X X,ZENG Y,et al.Dynamic change of bacterial community during dairy manure composting process revealed by high-throughput sequencing and advanced bioinformatics tools[J].Bioresource Technology,2020,306:123091.doi.org/10.1016/j.biortech.2020.123091.
[30]	徐杰,许修宏,门梦琪,等.木质纤维素降解菌剂DN-1促进堆肥腐熟度的评估[J].中国土壤与肥料,2016,266(6):146-151.
[31]	李秋芬,有小娟,张艳,等.象山港中部养殖区细菌群落结构的特征及其在生境修复过程中的变化[J].中国水产科学,2013,20(6):1234-1246.
[32]	黄玉杰,陈贯虹,张强,等.微生物除臭剂在畜禽粪便无害化处理中的应用进展[J].当代畜牧,2017(3):53-57.
[33]	NOLLA-ARDEVOL V,STROUS M,TEGETMEYER H E.Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions:biogas production,metagenome,and metatranscriptome[J].Frontiers in Microbiology,2015,6.doi.org/10.3389/fmicp.2015.00597.doi.org/10.3389/fmicp.2015.00597.
[34]	LI H Y,ZHENG X Q,CAO H Y,et al.Reduction of antibiotic resistance genes under different conditions during composting process of aerobic combined with anaerobic[J].Bioresource Technology,2021,325,124710.doi.org/10.1016/j.biortech.2021.124710.
[35]	MUJTABA G,RIZWAN M,LEE K.Removal of nutrients and COD from wastewater using symbiotic co-culture of bacterium Pseudomonas putida and immobilized microalga Chlorella vulgaris[J].Journal of Industrial and Engineering Chemistry,2017,49:145-151.
[36]	LI L Y,FENG J W,ZHANG L,et al.Enhanced nitrogen and phosphorus removal by natural pyrite-based constructed wetland with intermittent aeration[J].Environmental Science and Pollution Research,2021,28(48):69012-69028.
[37]	陈翰.进水有机物浓度对好氧颗粒污泥形成的影响机制[D].哈尔滨:哈尔滨工业大学,2019.
[38]	尤新新,王晟,都林娜.一种内源性复合微生物菌剂的特性及其固定化对猪场粪污水的处理效果[J].浙江大学学报(农业与生命科学版),2021,47(1):98-106.
[39]	李文兵,毕江涛,刘鹏,等.牛粪好氧堆肥发酵微生物群落结构演替与环境因子和腐熟度的相关性[J].环境工程学报,2022,40(1):69-77.
[40]	ZHANG C,YUAN C B,ZHU Y A,et al.A novel MABR process based on HN-AD bacteria-chlorella symbiotic system:effects of COD/TN ratios on performance,community structure,functional bacteria and key genes[J].Journal of Water Process Engineering,2022,49:103157.
[41]	WALLENSTEIN M D,MCMAHON S,SCHIMEL J.Bacterial and fungal community structure in Arctic tundra tussock and shrub soils[J].Fems Microbiology Ecology,2007,59(2):428-435.