NITROGEN REMOVAL EFFICIENCY AND CARBON SOURCE UTILIZATION CHARACTERISTICS OF PARTIAL DENITRIFICATION COUPLING ANAMMOX PROCESSES WITH DIFFERENT SLUDGE AGGREGATION MODES

LI Cong; DU Rui; PENG Yongzhen

doi:10.13205/j.hjgc.202309001

Volume 41 Issue 9

Sep. 2023

Turn off MathJax

Article Contents

Article Navigation > ENVIRONMENTAL ENGINEERING > 2023 > 41(9): 1-9

LI Cong, DU Rui, PENG Yongzhen. NITROGEN REMOVAL EFFICIENCY AND CARBON SOURCE UTILIZATION CHARACTERISTICS OF PARTIAL DENITRIFICATION COUPLING ANAMMOX PROCESSES WITH DIFFERENT SLUDGE AGGREGATION MODES[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(9): 1-9. doi: 10.13205/j.hjgc.202309001

Citation:

LI Cong, DU Rui, PENG Yongzhen. NITROGEN REMOVAL EFFICIENCY AND CARBON SOURCE UTILIZATION CHARACTERISTICS OF PARTIAL DENITRIFICATION COUPLING ANAMMOX PROCESSES WITH DIFFERENT SLUDGE AGGREGATION MODES[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(9): 1-9. doi: 10.13205/j.hjgc.202309001

Citation:

LI Cong, DU Rui, PENG Yongzhen. NITROGEN REMOVAL EFFICIENCY AND CARBON SOURCE UTILIZATION CHARACTERISTICS OF PARTIAL DENITRIFICATION COUPLING ANAMMOX PROCESSES WITH DIFFERENT SLUDGE AGGREGATION MODES[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(9): 1-9. doi: 10.13205/j.hjgc.202309001

PDF( 2343 KB)

NITROGEN REMOVAL EFFICIENCY AND CARBON SOURCE UTILIZATION CHARACTERISTICS OF PARTIAL DENITRIFICATION COUPLING ANAMMOX PROCESSES WITH DIFFERENT SLUDGE AGGREGATION MODES

doi: 10.13205/j.hjgc.202309001

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China

Received Date: 2023-07-19
Available Online: 2023-11-15

Abstract

Abstract

This study investigated the startup and long-term nitrogen removal efficiency, activities of functional bacteria, and microbial structure of partial denitrification coupling Anammox (PD/A) with biofilm and granular sludge. Batch tests were conducted to investigate the effect of the carbon source type and C/N(COD/NO₃^--N) on nitrogen removal efficiency of PD/A sludge with different sludge aggregation modes. Results showed that both biofilm and granular sludge systems could achieve efficient removal efficiency of ammonia (NH₄⁺-N) and nitrate (NO₃^--N), with total nitrogen (TN) removal efficiency of 90.6% and 96.2%, respectively. The carbon source type affected PD process achieving nitrite (NO₂^--N) accumulation obviously. The specific reduction rate of nitrate (μNO₃) in descending order was sodium acetate, glucose, ethanol and methanol, when C/N was 5.0 in the biofilm system. The specific reduction of ammonia (μNH₄) in descending order was glucose, ethanol, sodium acetate and methanol, when C/N was 3.0. The activity of the Anammox process increased with the activity of PD process under a suitable C/N. It held a strong PD process when the sodium acetate was the carbon source in the granular sludge system, however, it held a weaker PD process than biofilm system with the carbon source type changed. Therefore, selecting an appropriate carbon source and C/N for different sludge aggregation systems is beneficial for maintaining the stability of PD/A process.
- partial denitrification,
- anammox,
- biofilm,
- granular sludge,
- carbon source type

FullText(HTML)

References(30)

References

[1]	CAO S, DU R, LI B, et al. High-throughput profiling of microbial community structures in an ANAMMOX-UASB reactor treating high-strength wastewater[J]. Appl Microbiol Biotechnol, 2016, 100(14):6457-6467.
[2]	WANG Z, ZHENG M, DUAN H,et al.A 20-Year journey of partial nitritation and Anammox (PN/A):from sidestream toward mainstream[J].Environmental Science & Technology, 2022, 56(12):7522-7531.
[3]	JIA M, SOLON K, VANDEPLASSCHE D, et al. Model-based evaluation of an integrated high-rate activated sludge and mainstream anammox system[J/OL]. 2019.DOI: 10.1016/j.cej.2019.122878.
[4]	GUO Y, LI Y Y. Hydroxyapatite crystallization-based phosphorus recovery coupling with the nitrogen removal through partial nitritation/anammox in a single reactor[J]. Water Research, 2020, 187.DOI: 10.1016/j.watres.2020.116444.
[5]	LIU L, JI M, WANG F, et al. Insight into the influence of microbial aggregate types on nitrogen removal performance and microbial community in the anammox process:a review and meta-analysis[J]. The Science of the Total Environment, 2020, 714.DOI: 10.1016/j.scitotenv.2020.136571.
[6]	DU R, CAO S, PENG Y, et al. Combined partial denitrification (PD)-Anammox:a method for high nitrate wastewater treatment[J]. Environ International, 2019, 126:707-716.
[7]	DU R, CAO S, ZHANG H, et al. Flexible nitrite supply alternative for mainstream anammox:advances in enhancing process stability[J]. Environ Sci Technol, 2020, 54(10):6353-6364.
[8]	DU R, PENG Y. Technical revolution of biological nitrogen removal from municipal wastewater:recent advances in Anammox research and application[J]. Scientia Sinica Technologica, 2021, 52(3):389-402.
[9]	DU R, CAO S, JIN R, et al. Beyond an applicable rate in low-strength wastewater treatment by anammox:motivated labor at an extremely short hydraulic retention time[J]. Environ Sci Technol, 2022,54(12):6353-6364.
[10]	DU R, CAO S, LI B, et al. Simultaneous domestic wastewater and nitrate sewage treatment by Denitrifying Ammonium Oxidation (DEAMOX) in sequencing batch reactor[J]. Chemosphere, 2017, 174:399-407.
[11]	陈思宇,张绍青,陈鹏,等.基于短程反硝化的生物脱氮技术研究进展[J].环境工程,2021,39(5):38-44.
[12]	SHI L, DU R, PENG Y, et al. Successful establishment of partial denitrification by introducing hydrolytic acidification of slowly biodegradable organic matter[J/OL]. Bioresource Technology, 2020, 315(3):123887.DOI: 10.1016/j.biortech.2020.123887.
[13]	CAO S, LI B, DU R, et al. Nitrite production in a partial denitrifying upflow sludge bed (USB) reactor equipped with gas automatic circulation (GAC)[J]. Water Res, 2016, 90:309-316.
[14]	王维奇,王秀杰,李军,等.部分反硝化耦合厌氧氨氧化脱氮性能研究[J].中国环境科学,2019,39(2):641-647.
[15]	牛萌,王淑莹,杜睿,等.甲醇为碳源短程反硝化亚硝酸盐积累特性[J].中国环境科学,2017,37(9):3301-3308.
[16]	孙洪伟,王淑莹,王希明,等.低温SBR反硝化过程亚硝态氮积累试验研究[J].环境科学,2009,30(12):3619-3623.
[17]	LI J, PENG Y, GAO R, et al. Highly enriched anammox within anoxic biofilms by reducing suspended sludge biomass in a real-sewage A²/O process[J/OL]. Water Research,2021.DOI: 10.1016/j.watres.2021.116906.
[18]	LIN L, LUO Z, ISHIDA K, et al. Fast formation of anammox granules using a nitrification-denitrification sludge and transformation of microbial community[J]. Water Research, 2022, 221:118751.
[19]	LIU Y, NIU Q, WANG S, et al. Upgrading of the symbiosis of Nitrosomanas and anammox bacteria in a novel single-stage partial nitritation-anammox system:nitrogen removal potential and Microbial characterization[J]. Bioresource Technology, 2017, 244(Pt 1):463-472.
[20]	LIU Q, LI J, ZHAO Y, et al. Mechanism of suspended sludge impact on anammox enrichment in anoxic biofilm through long term operation and microbial analysis[J]. Water Research, 2022, 229:119412.
[21]	ZHANG Q, YANG G, SUN K, et al. Insights into the effects of bio-augmentation on the granule-based anammox process under continuous oxytetracycline stress:performance and microflora structure[J]. Chemical Engineering Journal, 2018, 348:503-513.
[22]	张树军,黄剑明,马淑勍,等.连续流分段进水短程反硝化-厌氧氨氧化耦合工艺的反硝化脱氮特性[J].环境工程,2022,40(11):13-18.
[23]	DU R, CAO S, LI X, et al. Efficient partial-denitrification/anammox (PD/A) process through gas-mixing strategy:system evaluation and microbial analysis[J]. Bioresource Technology, 2019, 300(5):122675.DOI: 10.1016/j.biortech.2019.122675.
[24]	ZHANG J, PENG Y, LI X, et al. Feasibility of partial-denitrification/anammox for pharmaceutical wastewater treatment in a hybrid biofilm reactor[J]. Water Research, 2022, 208.DOI: 10.1016/j.watres.2021.117856.
[25]	FAN Z, ZENG W, LIU H, et al. A novel partial denitrification, anammox-biological phosphorus removal, fermentation and partial nitrification (PDA-PFPN) process for real domestic wastewater and waste activated sludge treatment[J]. Water Res, 2022, 217. DOI: 10.1016/j.watres.2022.118376.
[26]	GONG L, HUO M, YANG Q, et al. Performance of heterotrophic partial denitrification under feast-famine condition of electron donor:a case study using acetate as external carbon source[J]. Bioresour Technol, 2013, 133:263-269.
[27]	LI C, LIU Q, FAN J, et al. Metagenomics-based interpretation of selective bioaugmentation promoting partial-denitrification coupling with anammox process reactivation in suspended sludge system[J]. Chem Eng J, 2023, 454.
[28]	LI X, PENG Y, ZHANG J, et al. Multiple roles of complex organics in polishing THP-AD filtrate with double-line anammox:inhibitory relief and bacterial selection[J]. Water Res, 2022, 216:118373.
[29]	WANG D, MENG Y, MENG F. Genome-centric metagenomics insights into functional divergence and horizontal gene transfer of denitrifying bacteria in anammox consortia[J]. Water Res, 2022, 224:119062.
[30]	GAO R, PENG Y, LI J, et al. Mainstream partial denitrification-anammox (PD/A) for municipal sewage treatment from moderate to low temperature:reactor performance and bacterial structure[J]. Science of the Total Environment, 2022, 806:150267.DOI: 10.1016/j.scitotenv.2021.150267.