CHARACTERISTICS OF DENITRIFICATION PHOSPHORUS REMOVAL AND N<sub>2</sub>O EMISSION IN AN/A/O-SBR UNDER DIFFERENT TEMPERATURES

REN Lifang; LI Xiaoqing; SUN Hongwei

doi:10.13205/j.hjgc.202312013

Volume 41 Issue 12

Dec. 2023

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2023 > 41(12): 107-115

REN Lifang, LI Xiaoqing, SUN Hongwei. CHARACTERISTICS OF DENITRIFICATION PHOSPHORUS REMOVAL AND N2O EMISSION IN AN/A/O-SBR UNDER DIFFERENT TEMPERATURES[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(12): 107-115. doi: 10.13205/j.hjgc.202312013

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REN Lifang, LI Xiaoqing, SUN Hongwei. CHARACTERISTICS OF DENITRIFICATION PHOSPHORUS REMOVAL AND N₂O EMISSION IN AN/A/O-SBR UNDER DIFFERENT TEMPERATURES[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(12): 107-115. doi: 10.13205/j.hjgc.202312013

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CHARACTERISTICS OF DENITRIFICATION PHOSPHORUS REMOVAL AND N₂O EMISSION IN AN/A/O-SBR UNDER DIFFERENT TEMPERATURES

doi: 10.13205/j.hjgc.202312013

1. Department of Architecture Engineering, Yantai Vocational College, Yantai 264670, China;
2. Yantai Vocational College of Culture Tourism, Yantai 264000, China;
3. School of Environmental and Material Engineering, Yantai University, Yantai 264005, China

Received Date: 2022-09-02
Available Online: 2024-03-08

Abstract

Abstract

Using an anaerobic/anoxic/aerobic sequencing batch reactor (An/A/O-SBR), the long-term impact of temperature (T=32, 27, 22, 17,12 ℃) on denitrification phosphorus removal performance and N₂O emission characteristics was investigated with five stages. The characteristics of anoxic nitrogen and phosphorus removal, as well as N₂O release in the system under different temperature conditions were investigated. Based on the analysis of the degradation characteristics of microbial flora, the competitive characteristics between the phosphorus accumulating bacteria (PAOs) and the glycogen accumulating bacteria (GAOs) were determined under different temperatures as well. The results showed that the denitrification and phosphorus removal performance of An/A/O-SBR increased first and then decreased with the temperature decreasing. The NO_x^- and TP removal efficiencies reached 95.5% and 90.3%, respectively, and the N₂O yield decreased to 3.71% at 22 ℃. To some extent, the low temperature promoted the competitive advantage of PAOs. The proportion of PHB in the synthetic PHA during the anaerobic stage (ΔPHB/ΔPHA), the proportion of PHB consumed in PHA (PHB_con/PHA_con), the proportion of glycogen synthesized in PHA consumed during the anoxic (Gly_in/PHA_con) were gradually close to the degradation characteristics of PAOs. The denitrification process of GAOs does not absorb excessive phosphorus, resulting in the reduction of TP removal efficiency. Under lower temperatures, both the enzymatic reaction rate and the electron providing rate by PHA decreased, which led to the decrease of NO₃^- removal rate and the increase of N₂O yield in the anoxic stage. Higher temperatures promoted the proliferation of GAOs in the An/A/O-SBR system.
- temperature,
- denitrifying phosphorus accumulating organisms,
- glycogen accumulating organisms,
- denitrifying phosphorus removal process,
- N₂O

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References

[1]	CAMEJO P Y, OWEN B R, MARTIRANO J, et al. Candidatus accumulibacter phosphatis clades enriched under cyclic anaerobic and microaerobic conditions simultaneously use different electron acceptors[J]. Water Research, 2016,102(1): 125-137.
[2]	FAN Z, ZENG W, MENG Q, et al. Achieving enhanced biological phosphorus removal utilizing waste activated sludge as sole carbon source and simultaneous sludge reduction in sequencing batch reactor[J]. Science of the Total Environment, 2021,799(10): 149291.
[3]	LI Y, RAHMAN S M, LI G, et al. The composition and implications of polyphosphate-metal in enhanced biological phosphorus removal systems[J]. Environmental Science & Technology, 2019,53(3): 1536-1544.
[4]	JI J T, PENG Y Z, WANG B, et al. A novel SNPR process for advanced nitrogen and phosphorus removal from main stream wastewater based on anammox, endogenous partial-denitrification and denitrifying dephosphatation[J]. Water Research, 2020,170(1): 115363.
[5]	MENG Q G, ZENG W, WANG B G, et al. New insights in the competition of polyphosphate-accumulating organisms and glycogen-accumulating organisms under glycogen accumulating metabolism with trace Poly-P using flow cytometry[J]. Chemical Engineering Journal,2020,385(1):123915.
[6]	BAI X, MCK M M, NEUFELD J D. Nitrogen removal pathways during simultaneous nitrification, denitrification, and phosphorus removal under low temperature and dissolved oxygen conditions[J] Bioresource Technology, 2022, 354: 127177.
[7]	KAO C, LI J, GAO R, et al. Advanced nitrogen removal from real municipal wastewater by multiple coupling nitritation, denitritation and endogenous denitritation with anammox in a single suspended sludge bioreactor[J]. Water Research, 2022, 221(1): 118749.
[8]	YUAN C, WANG B, PENG Y, et al. Enhanced nutrient removal of simultaneous partial nitrification, denitrification and phosphorus removal (SPNDPR) in a single-stage an aerobic/micro-aerobic sequencing batch reactor for treating real sewage with low carbon/nitrogen[J]. Chemosphere, 2020,257:127097.
[9]	WANG Y, GUO G, WANG H, et al. Long-term impact of an aerobic reaction time on the performance and granular characteristics of granular denitrifying biological phosphorus removal systems[J]. Water Research, 2013,47(14): 5326-5337.
[10]	巩有奎, 李美玲, 孙洪伟. 不同NO^-₃浓度An/A/O-SBR系统PAOs-GAOs竞争及N₂O释放特性[J].化工学报, 2021,72(3): 1675-1683.
[11]	ZHANG S. HUANG Y, HUA Y. Denitrifying dephosphatation over nitrite:effects of nitrite concentration, organic carbon, and pH[J]. Bioresource Technology, 2010,101(11):3870-3875.
[12]	PANSWAD T, DOUNG C A, JIN A. Temperature effect on microbial community of enhanced biological phosphorus removal system[J]. Water Research, 2003, 37(2):409-415.
[13]	LIU H, ZENG W, MENG Q, et al. Phosphorus removal performance, intracellular metabolites and clade-level community structure of Tetrasphaera-dominated polyphosphate accumulating organisms at different temperatures[J]. Science of the Total Environment, 2022,842(10):156913.
[14]	WANG B, ZENG W, FAN Z, et al. Effects of polyaluminium chloride addition on community structures of polyphosphate and glycogen accumulating organisms in biological phosphorus removal (BPR) systems[J]. Bioresource Technology,2019, 297, 122431.
[15]	APHA (American Public Health Association). Standard Methods for the Examination of Water and Wastewater. Baltimore[M]. Port City Press, 1998.
[16]	YANG Q, LIU X H, PENG C Y, et al. N₂O production during nitrogen removal via nitrite from domestic wastewater: main sources and control method[J]. Environmental Science & Technology, 2009, 43(24): 9400-9406.
[17]	OEHMEN A, KELLER L. B, ZENG R J, et al. Optimisation of poly-beta-hydroxyalkanoate analysis using gas chromatography for enhanced biological phosphorus removal systems[J]. Journal of Chromatography A, 2005, 1070(1/2): 131-136.
[18]	OEHMEN A, ZENG R J, YUAN Z, et al. Anaerobic metabolism of propionate by polyphosphate-accumulating organisms in enhanced biological phosphorus removal systems[J]. Biotechnology and Bioengineering, 2005, 91(1): 43-53.
[19]	SOTO O, ROECKEL M. Kinetics of cross-inhibited denitrification of a high load wastewater[J]. Enzyme Microb Tech, 2007, 40(6): 1627-1634.
[20]	NODA N, KANEKO N, MIKAMI M, et al. Effects of SRT and DO on N₂O reductase activity in an anoxic-oxic activated sludge system[J]. Wat Sci Technol, 2003,48(11/12):363-370.
[21]	BRDJANOVIC D, LOOSDRECHT M C M V, HOOIJMANS C M, et al. Temperature effects on physiology of biological phosphorus removal[J]. Journal of Environmental Engineering, 1997, 123(2): 144-152.
[22]	RIBERA-GUARDIA A, MARQUES R, ARANGIO C, et al. Distinctive denitrifying capabilities lead to differences in N₂O production by denitrifying polyphosphate accumulating organisms and denitrifying glycogen accumulating organisms[J]. Bioresource Technology, 2016, 219: 106-113.
[23]	OEHMEN A, CARVALHO G, LOPEZ C M, et al. Incorporating microbial ecology into the metabolic modelling of polyphosphate accumulating organisms and glycogen accumulating organisms[J]. Water Research, 2010,44(17):4992-5004.
[24]	WANG X, WANG S, ZHAO J, et al. A novel stoichio-metries methodology to quantify functional microor-ganisms in simultaneous nitrification-endogenous denitrification and phosphorous removal (SNEDPR)[J]. Water Research, 2015, 95(15): 319-329.
[25]	THIRD K A, BURNETT N, CORD-RUWISCH R, Simultaneous nitrification and denitrification using stored substrate (PHB) as the electron donor in an SBR[J]. Biotechnology Bioengeering, 2003, 83(6): 706-720.
[26]	ZENG W, BAI X L, GUO Y, et al. Interaction of "Candidatus accumulibacter" and nitrifying bacteria to achieve energy efficient denitrifying phosphorus removal via nitrite pathway from sewage[J]. Enzyme and Microbial Technology, 2017,105(1):1-8.
[27]	YUAN C S, WANH B, PENG Y Z, et al. Enhanced nutrient removal of simultaneous partial nitrification, denitrification and phosphorus removal (SPNDPR) in a single-stage anaerobic/micro-aerobic sequencing batch reactor for treating real sewage with low carbon/nitrogen[J]. Chemosphere, 2020,257:127097.
[28]	ZHOU Y, PIJUAN M, ZENG R J, et al. Free nitrous acid inhibition on nitrous oxide reduction by a denitrifying enhanced biological phosphorus removal sludge[J]. Environmental Science & Technology, 2008, 42(22): 8260-8265.
[29]	MASSARA T M, MALAMIS S, GUISASOLA A, et al. A review on nitrous oxide (N₂O) emissions during biological nutrient removal from municipal wastewater and sludge reject water[J]. Science of the Total Environment, 2017,596/597(1):106-123.
[30]	马娟,宋相蕊,李璐. 碳源对反硝化过程NO^-₂积累及出水pH值的影响[J].中国环境科学. 2014,34(10): 2556-2561.
[31]	WEI Y, WANG S Y, MA B, et al. The effect of poly-β-hydroxyalkanoates degradation rate on nitrous oxide production in a denitrifying phosphorus removal system[J]. Bioresource Technology, 2014, 170(1): 175-182.
[32]	ZHOU Y, OEMEN A, LIM M, et al. The role of nitrite and free nitrous acid (FNA) in wastewater treatment plants[J]. Water Research, 2011, 45(15): 4672-4682.
[33]	PAUDEL S, OHKYUAK, SAMIR. Effects of temperature on nitrous oxide (N₂O) emission from intensive aquaculture system[J]. Applied Economic Perspectives and Policy, 2015,518(1):16-23.
[34]	NEMETH D D, WAGNER-RIDDLE C, DUNFIELD K E. Abundance and gene expression in nitrifier and denitrifier communities associated with a field scale spring thaw N₂O flux event[J]. Soil Biology Biochemistry,2014,73(1): 1-9.
[35]	KIM S W,MIYAHARA M, FUSHINOBU S, et al. Nitrous oxide emission from nitrifying activated sludge dependent on denitrification by ammonia-oxidizing bacteria[J]. Bioresource Technology,2010,101(11):3958-3963.