SCREENING, IDENTIFICATION, AND VALIDATION OF FUNCTIONAL BACTERIAL STRAINS IN DENITRIFICATION AND DESULFURIZATION PROCESS

XU Ran; CUI Jiandong; GAO Shuang; HUANG Cong

doi:10.13205/j.hjgc.202312015

Volume 41 Issue 12

Dec. 2023

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2023 > 41(12): 123-130

XU Ran, CUI Jiandong, GAO Shuang, HUANG Cong. SCREENING, IDENTIFICATION, AND VALIDATION OF FUNCTIONAL BACTERIAL STRAINS IN DENITRIFICATION AND DESULFURIZATION PROCESS[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(12): 123-130. doi: 10.13205/j.hjgc.202312015

Citation:

XU Ran, CUI Jiandong, GAO Shuang, HUANG Cong. SCREENING, IDENTIFICATION, AND VALIDATION OF FUNCTIONAL BACTERIAL STRAINS IN DENITRIFICATION AND DESULFURIZATION PROCESS[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(12): 123-130. doi: 10.13205/j.hjgc.202312015

Citation:

XU Ran, CUI Jiandong, GAO Shuang, HUANG Cong. SCREENING, IDENTIFICATION, AND VALIDATION OF FUNCTIONAL BACTERIAL STRAINS IN DENITRIFICATION AND DESULFURIZATION PROCESS[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(12): 123-130. doi: 10.13205/j.hjgc.202312015

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SCREENING, IDENTIFICATION, AND VALIDATION OF FUNCTIONAL BACTERIAL STRAINS IN DENITRIFICATION AND DESULFURIZATION PROCESS

doi: 10.13205/j.hjgc.202312015

1. School of Biological Engineering, Tianjin University of Science and Technology, Tianjin 300457, China;
2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China

Received Date: 2023-03-29
Available Online: 2024-03-08

Abstract

Abstract

The denitrifying sulfur oxidation process is one of the most potential sewage treatment technologies in biological treatment for treating sulfur-containing and nitrogen-containing organic wastewater. In order to understand the information of bacteria that mainly play a role in the denitrifying sulfur oxidation process, three expanded granular sludge bed reactors were operated simultaneously to determine the oxidation efficiency of sulfur denitrification, analyze the changes of the microbial community structure of activated sludge, isolate and screen out the strains in activated sludge and verify their function. The results showed that 100% of 100 mg/L NO₃^--N and 100 mg/L Ac^--C can be removed after stable operation of the three reactors, and up to 90% of 200 mg/L S^2- can be removed. 16S rRNA analysis was used to analyze the microbial community structure and diversity of activated sludge in the stable operation reactor, and found that the five genera with high relative abundance were Azoarcus, Pseudomonas, Thauera, Arthrobacter and Desulfomicrobium. A total of 50 strains belonging to 19 genera were obtained by plate separation and flow cytometry separation, and the removal rate of carbon, nitrogen and sulfur pollutants was synthesized to preliminarily determine that Azoarcus, Thauera, Pseudomonas, Acinetobacter and Agrobacterium were the functional genera of denitrified sulfur oxidation process.

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References(17)

References

[1]	苏柏懿,吴莉娜,王春艳,等.硫自养反硝化在工业废水处理中的研究进展[J].应用化工,2022,51(4):1070-1076.
[2]	HUANG C, LIU Q, LI Z L, et al. Relationship between functional bacteria in a denitrification desulfurization system under autotrophic, heterotrophic, and mixotrophic conditions[J]. Water Research,2021,188:116526.
[3]	XU X J, CHEN C, GUO H, et al. Characterization of a newly isolated strain Pseudomonas sp. C27 for sulfide oxidation: reaction kinetics and stoichiometry[J]. Scientific Reports,2016,6(1):1-10.
[4]	WANG A J, DU D Z, REN N Q, et al. An innovative process of simultaneous desulfurization and denitrification by Thiobacillus denitrificans[J]. Journal of Environmental Science and Health,2005, 40(10):1939-1949.
[5]	HUANG C, LIU W Z, LI Z L, et al. High recycling efficiency and elemental sulfur purity achieved in a biofilm formed membrane filtration reactor[J]. Water Research,2018,130:1-12.
[6]	ZHANG R C, XU X J, CHEN C, et al. Interactions of functional bacteria and their contributions to the performance in integrated autotrophic and heterotrophic denitrification[J]. Water Research,2018,143:355-366.
[7]	TAN W B, HUANG C, CHEN C, et al. Bioaugmentation of activated sludge with elemental sulfur producing strain Thiopseudomonas denitrificans X2 against nitrate shock load[J]. Bioresource Technology,2016,220:647-650.
[8]	PISHGAR R, DOMINIC J A, SHENG Z, et al. Denitrification performance and microbial versatility in response to different selection pressures[J]. Bioresource Technology,2019,281:72-83.
[9]	HUANG C, LIU Q, CHEN X Q, et al. Bioaugmentation with Thiobacillus sp. H1 in an autotrophic denitrification desulfurization microbial reactor: microbial community changes and relationship[J]. Environmental Research,2020,189:109927.
[10]	HUANG S, YU D S, CHEN G H, et al. Realization of nitrite accumulation in a sulfide-driven autotrophic denitrification process: simultaneous nitrate and sulfur removal[J]. Chemosphere,2021,278:130413.
[11]	张若晨.自养—异养联合反硝化系统中功能菌群互作规律及代谢机制[D].哈尔滨:哈尔滨工业大学,2019.
[12]	陈雪琪.废水脱硫脱氮工艺效能调控及生物强化策略研究[D].哈尔滨:哈尔滨工业大学,2020.
[13]	SPEIRS L B M, RICE D T F, PETROVSKI S, et al. The phylogeny, biodiversity, and ecology of the Chloroflexi in activated sludge[J]. Frontiers in Microbiology, 2019,10:2015.
[14]	马晓丹.脱硫脱氮细菌的分离筛选及其生物强化效能研究[D]. 哈尔滨:哈尔滨工业大学,2015.
[15]	包海花.高效反硝化细菌的筛选及在污水脱氮中的应用[D].郑州:河南农业大学,2022.
[16]	秦亚玲,梁宗林,宋阳,等.高通量测序分析云南腾冲热海热泉微生物多样性[J].微生物学通报,2019,46(10):2482-2493.
[17]	高健.生物反硝化硫资源化颗粒污泥技术特性及其微生态机理[D].上海:华东理工大学,2020.