ISOLATION AND IDENTIFICATION OF A NEW SULFATE-REDUCING BACTERIUM AND ITS <i>IN SITU</i> REMEDIATION EFFECT OF HEXAVALENT CHROMIUM-CONTAMINATED SOIL

HAN Jianjun; CHAI Lujun; WANG Guojin; ZHANG Yu; QIN Kangjia; ZHOU Man; LIANG Xuejie; HAO Junpeng; WANG Hui

doi:10.13205/j.hjgc.202402023

Volume 42 Issue 2

Feb. 2024

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2024 > 42(2): 192-198

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HAN Jianjun, CHAI Lujun, WANG Guojin, ZHANG Yu, QIN Kangjia, ZHOU Man, LIANG Xuejie, HAO Junpeng, WANG Hui. ISOLATION AND IDENTIFICATION OF A NEW SULFATE-REDUCING BACTERIUM AND ITS IN SITU REMEDIATION EFFECT OF HEXAVALENT CHROMIUM-CONTAMINATED SOIL[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(2): 192-198. doi: 10.13205/j.hjgc.202402023

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ISOLATION AND IDENTIFICATION OF A NEW SULFATE-REDUCING BACTERIUM AND ITS IN SITU REMEDIATION EFFECT OF HEXAVALENT CHROMIUM-CONTAMINATED SOIL

doi: 10.13205/j.hjgc.202402023

1. Research Institute for Environmental Innovation (Suzhou) Tsinghua, Suzhou 215010, China;
2. Beijing Bennong Technology Development Co., Ltd, Beijing 100176, China;
3. Shangdong Branch of China National Geological Exploration Center of Building Materials Industry, Jinan 250100, China;
4. Tsinghua University, Beijing 100091, China

Received Date: 2021-05-28
Available Online: 2024-04-28

Abstract

Abstract

Microbial reduction of Cr(Ⅵ) to Cr(Ⅲ) is a promising method for remediation of chromium-contaminated soil. Molecular biology was used to identify the species of the strain, and the in situ remediation effect of hexavalent chromium was evaluated through laboratory tests and physical simulation experiments. The results showed that the 16S rDNA full-length sequence of the isolated strain capable of reducing Cr(Ⅵ) was analyzed in Ezbiocloud data, and the similarity was 98.89% with Desulfovibrio desulfuricans DSM 642T, named D. desulfuricans S-7. The most suitable growth temperature for D. desulfuricans S-7 was 30 ℃, while the most suitable pH was 7.0, and the suitable NaCl concentration was 0 to 10%. The results showed that the removal rate of 25 mg/L Cr(Ⅵ) was 79.74%, and the growth and reproduction of the strain was inhibited under an initial 100 mg/L of Cr(Ⅵ). The simulation experiment results revealed Cr(Ⅵ) concentration in the soil changed from 50.0 mg/kg to 3.1 mg/kg, and its removal rate reached 93.8%, while the remediated soil could meet China's national standard, GB 36600—2018, for Class 1 development land. This study showed that D. Desulfurians S-7 can effectively remediate Cr(Ⅵ) contaminated soil in anaerobic environment, providing a new alternative for in-situ remediation of deep-layer Cr(Ⅵ) contaminated soil.
- sulfate-reducing bacteria,
- Desulfovibrio desulfuricans,
- hexavalent chromium,
- soil pollution,
- in situ remediation

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