CHARACTERISTICS OF DENITRIFYING PHOSPHORUS REMOVAL AND N<sub>2</sub>O EMISSION OF AN AOA-SBR UNDER DIFFERENT CARBON TO NITROGEN RATIOS (C/N)

REN Lifang; GONG Youkui; SUN Hongwei

doi:10.13205/j.hjgc.202405001

Volume 42 Issue 5

May 2024

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REN Lifang, GONG Youkui, SUN Hongwei. CHARACTERISTICS OF DENITRIFYING PHOSPHORUS REMOVAL AND N2O EMISSION OF AN AOA-SBR UNDER DIFFERENT CARBON TO NITROGEN RATIOS (C/N)[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(5): 1-9. doi: 10.13205/j.hjgc.202405001

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REN Lifang, GONG Youkui, SUN Hongwei. CHARACTERISTICS OF DENITRIFYING PHOSPHORUS REMOVAL AND N₂O EMISSION OF AN AOA-SBR UNDER DIFFERENT CARBON TO NITROGEN RATIOS (C/N)[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(5): 1-9. doi: 10.13205/j.hjgc.202405001

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CHARACTERISTICS OF DENITRIFYING PHOSPHORUS REMOVAL AND N₂O EMISSION OF AN AOA-SBR UNDER DIFFERENT CARBON TO NITROGEN RATIOS (C/N)

doi: 10.13205/j.hjgc.202405001

1. Department of Architecture Engineering, Yantai Vocational College, Yantai 264670, China;
2. School of Environmental and Material Engineering, Yantai University, Yantai 264005, China

Received Date: 2023-11-12
Available Online: 2024-07-11

Abstract

Abstract

In the paper, the simultaneous nitrogen and phosphorus removal process (SNDPR) of domestic sewage was accomplished in the anaerobic/aerobic/anaerobic SBR (AAO-SBR) by gradually reducing the external carbon source addition, to reduce the carbon nitrogen ratio (C/N) of influent water, reducing the aerobic aeration rate, and increasing the residence time of aerobic and anoxic stages. The competition between PAOs and GAOs in AOA-SBR under different reaction conditions was investigated by the stoichiometric method, and the nitrogen and phosphorus removal performance, as well as the N₂O emission ratio of the system were determined. The results showed that reducing the aerobic aeration rate and increasing the aerobic residence time were conducive to the proliferation of PAOs in AOA-SBR under the condition of low carbon to nitrogen ratio, which promoted denitrification and phosphorus removal. When the C/N ratio decreased from 7.0 to 3.3, the average TN removal rate reached more than 80%, and the average TP removal rate increased from 65.2% to 81.2%. Under different C/N conditions, the changes of microbial endogenous substances in AOA-SBR at anaerobic, anoxic and aerobic stages showed the coexistence characteristics of PAOs-GAO. AOA-SBR tended to enrich the characteristics of GAOs endogenous substances at a higher C/N. Higher C/N condition promoted the endogenous denitrification process of DGAOs and increased the release of N₂O, and lower C/N promoted the proliferation of DPAOs. The coupled endogenous denitrification process of DGAOs-DGAOS promoted the reduction of N₂O, which reduced the emission of N₂O. As the C/N decreased from 7.0 to 3.3, the N₂O release decreased from 2.23 to 1.05 mg/L, and the emission ratio decreased from 7.21% to 3.94%. The collaboration between DGAOs endogenous denitrification and DPAOs endogenous denitrification phosphorus removal processes can make full use of carbon sources in raw domestic sewage, which may break the bottleneck of nitrogen removal in urban domestic sewage.
- carbon to nitrogen ratio,
- AOA-SBR,
- simultaneous nitrification and denitrification phosphorous removal process (SNDPR),
- N₂O emission

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References(33)

References

[1]	HUANG X, ZHU J, DUAN W Y, et al. Biological nitrogen removal and metabolic characteristics in a full-scale two-staged anoxic-oxic(A/O) system to treat optoelectronic wastewater[J]. Bioresource Technology, 2020, 300: 122595.
[2]	李易寰,奚蕾蕾,钟奕杰,等. 倒置A²/O工艺运行效果及优化控制方案[J]. 环境工程,2020,38(3):76-81.
[3]	赵伟华,王梅香,李健伟,等. A²O工艺和A²O+BCO工艺的脱氮除磷性能比较[J]. 中国环境科学,2019,39(3):994-999.
[4]	陈诗,彭来,徐一峰,等. 废水生物脱氮过程中N₂O排放数学模型研究进展[J]. 环境工程,2022,40(6):97-106 ,122.
[5]	康华,李红艳,龙北生,等.改进型A₂NSBR工艺参数及其除磷脱氮特性[J].环境工程,2023,41(4):123-130.
[6]	LUO Y H, YI K, ZHANG X Y, et al. Simultaneous partial nitrification, denitrification, and phosphorus removal in sequencing batch reactors via controlled reduced aeration and short-term sludge retention time decrease[J]. Journal of Environmental Management,2023,344:118598.
[7]	LI D Y, GUO W, LIANG D B, et al. Rapid start-up and advanced nutrient removal of simultaneous nitrification, endogenous denitrification and phosphorus removal aerobic granular sequence batch reactor for treating low C/N domestic wastewater[J]. Environmental Research, 2022,212:113464.
[8]	RUBIO-RINCON F J, LOPEZ-VAZQUES C M, WELLES L, et al. Cooperation between Candidatus competibacter and Candidatus accumulibacter clade Ⅰ, in denitrification and phosphate removal processes[J]. Water Research, 2017, 120: 156-164.
[9]	FAN Z W, ZENG W, WANG B G, et al. Microbial community at transcription level in the synergy of GAOs and Candidatus accumulibacter for saving carbon source in wastewater treatment[J]. Bioresource Technology, 2020, 297: 122454.
[10]	WANG X X, WANG S Y, ZHAO J, et al. Combining simultaneous nitrification-endogenous denitrification and phosphorus removal with post-denitrification for low carbon/nitrogen wastewater treatment[J]. Bioresource Technology, 2016, 220: 17-25.
[11]	WANG X X, WANG S Y, XUE T L,et al.Treating low carbon/nitrogen (C/N) wastewater in simultaneous nitrification-endogenous denitrification and phosphorous removal (SNDPR) systems by strengthening anaerobic intracellular carbon storage[J]. Water Research, 2015,77:191-200.
[12]	SUN Z Y, LV Y K, LIU Y X, et al. Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a novel metal resistant bacterium Cupriavidus sp. S1[J]. Bioresource Technology, 2016,220:142-150.
[13]	ZHANG J H, ZHANG Q, LI X Y, et al. Rapid start-up of partial nitritation and simultaneously phosphorus removal (PNSPR) granular sludge reactor treating low-strength domestic sewage[J]. Bioresource Technology, 2017,243:660-666.
[14]	HU T T, PENG Y Z, YUAN C S, et al. Enhanced nutrient removal and facilitating granulation via intermittent aeration in simultaneous partial nitrification endogenous denitrification and phosphorus removal (SPNEDpr) process[J]. Chemosphere, 2021,285:131443.
[15]	RIBERA-GUARDIA A, MARQUES R, ARANGIO C, et al. Distinctive denitrifying capabilities lead to differences in N₂O production by denitrifying polyphosphate accumulating organisms and denitrifying glycogen accumulating organisms[J]. Bioresource Technology, 2016, 219: 106-113.
[16]	APHA(American Public Health Association). Standard Methods for the Examination of Water and Wastewater[M]. Baltimore: Port City Press, 1998.
[17]	OEHMEN A, KELLER B, ZENG R J, et al. Optimisation of poly-beta-hydroxyalkanoate analysis using gas chromatography for enhanced biological phosphorus removal systems[J]. Journal of Chromatography A, 2005, 107(1/2): 131-136.
[18]	OEHMEN A, ZENG R J, YUAN Z, et al. Anaerobic metabolism of propionate by polyphosphate-accumulating organisms in enhanced biological phosphorus removal systems[J]. Biotechnology and Bioengineering, 2005, 91(1): 43-53.
[19]	YANG Q, LIU X H, PENG C Y, et al. N₂O production during nitrogen removal via nitrite from domestic wastewater: main sources and control method[J]. Environmental Science & Technology, 2009,43(24): 9400-9406.
[20]	CARVALHEIRA M, OEHEMEN A, CARVALHO G, et al. Survival strategies of polyphosphate accumulating organisms and glycogen accumulating organisms under conditions of low organic loading[J]. Bioresource Technology, 2014,172: 290-296.
[21]	DAN Q P, PENG Y Z, WANG B, et al. Side-stream phosphorus famine selectively strengthens glycogen accumulating organisms (GAOs) for advanced nutrient removal in an anaerobic-aerobic-anoxic system[J]. Chemical Engineering Journal, 2021,420:129554.
[22]	SMOLDERS G J F, VANDERMEIJ J, van LOOSDRECHT M C M, et al. Model of the anaerobic metabolism of the biological phosphorus removal process: stoichiometry and pH influence[J]. Biotechnology and Bioengineering, 1994,43(6): 461-470.
[23]	ZENG R J, SAUNDERS A M, YUAN Z, et al. Identification and comparison of aerobic and denitrifying polyphosphate-accumulating organisms[J]. Biotechnology and Bioengineering, 2003,83(2): 140-148.
[24]	ZENG R J,VAN LOOSDRECHT M C M, YUAN Z, et al. Metabolic model for glycogen-accumulating organisms in anaerobic/aerobic activated sludge systems[J]. Biotechnology and Bioengineering, 2003,81(1):92-105.
[25]	IZADI P, IZADI P, ELDYASTI A. Understanding microbial shift of enhanced biological phosphorus removal process (EBPR) under different dissolved oxygen (DO) concentrations and hydraulic retention time (HRTs)[J]. Biochemical Engineering Journal,2021,166:107833.
[26]	LIU W T, NAKAMURA K, MATSUO T, et al. Internal energy-based competition between polyphosphate- and glycogen- accumulating bacteria in biological phosphorus removal reactors-effect of P/C feeding ratio[J]. Water Research, 2015,31(6): 1430-1438.
[27]	ZHAO J, WANG X X, LI X Y, et al. Combining partial nitrification and post endogenous denitrification in an EBPR system for deep-level nutrient removal from low carbon/nitrogen (C/N) domestic wastewater[J]. Chemosphere,2018,210: 19-28.
[28]	MAJED N, GU A Z. Phenotypic dynamics in polyphosphate and glycogen accumulating organisms in response to varying influent C/P ratios in EBPR systems[J]. Science of the Total Environment, 2020, 743(7): 140603.
[29]	SGUANCI S, LUBELLO C, CAFFAZ S, et al. Long-term stability of aerobic granular sludge for the treatment of very low-strength real domestic wastewater[J]. Journal of Cleaner Production, 2019,222: 882-890.
[30]	WEISSBRODT D G, SCHNEITER G S, FUERBRINGCR J M, et al. Identification of trigger factors selecting for polyphosphate- and glycogen-accumulating organisms in aerobic granular sludge sequencing batch reactors[J]. Water Research, 2013,47(19): 7006-7018.
[31]	OEHMEN A, SAUNDERS A M, VIVES M T, et al. Competition between polyphosphate and glycogen accumulating organisms in enhanced biological phosphorus removal systems with acetate and propionate as carbon sources[J]. Journal of Biotechnology, 2006,123(1): 22-32.
[32]	YUAN C S, WANG B, PENG Y Z, et al. Enhanced nutrient removal of simultaneous partial nitrification, denitrification and phosphorus removal (SPNDPR) in a single-stage anaerobic/micro-aerobic sequencing batch reactor for treating real sewage with low carbon/nitrogen[J]. Chemosphere, 2020, 257: 127097.
[33]	ZHOU Y, PIJUAN M, ZENG R J, et al. Free nitrous acid inhibition on nitrous oxide reduction by a denitrifying-enhanced biological phosphorus removal sludge[J]. Environmental Science and Technology, 2008, 42(22):8260-8265.